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Using in vivo oxidation status of one- and two-component redox relays to determine H2O2 levels linked to signaling and toxicity.

Autor: Domènech, Alba¹, Ayté, José¹, Antunes, Fernando² fantunes@fc.ul.pt, Hidalgo, Elena¹ elena.hidalgo@upf.edu

Publikováno v: BMC Biology. 6/1/2018, Vol. 16 Issue 1, p1-15. 15p.

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Lack of a peroxiredoxin suppresses the lethality of cells devoid of electron donors by channelling electrons to oxidized ribonucleotide reductase.

Autor: Boronat, Susanna¹, Domènech, Alba¹, Carmona, Mercè¹, García-Santamarina, Sarela¹, Bañó, M. Carmen², Ayté, José¹ jose.ayte@upf.edu, Hidalgo, Elena¹ elena.hidalgo@upf.edu

Publikováno v: PLoS Genetics. 6/22/2017, Vol. 13 Issue 6, p1-21. 21p.

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Additional file 4: of Using in vivo oxidation status of one- and two-component redox relays to determine H2O2 levels linked to signaling and toxicity

Autor: Domènech, Alba, Ayté, José, Antunes, Fernando, Hidalgo, Elena

Figure S4. Both Tpx1 and Ctt1 participate in peroxide scavenging at sub-toxic doses of H2O2, while only catalase acts as a scavenger of the toxic ones. The concentration of the remaining extracellular peroxides of MM cultures of strains 972 (WT), SG4

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47e58d61ff0d37ffc4cbea86b45fe2e3

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Additional file 6: of Using in vivo oxidation status of one- and two-component redox relays to determine H2O2 levels linked to signaling and toxicity

Autor: Domènech, Alba, Ayté, José, Antunes, Fernando, Hidalgo, Elena

Figure S6. At non-toxic concentrations of extracellular peroxides, both systems, Pap1-Tpx1 and OxyR, behave similarly. a Growth curves of strain AD29 (WT + HA-OxyR), treated or not with 0.1 mM or 0.5 mM H2O2, were recorded for 20 h. b Cultures of str

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1679cc936e282574dcfde19707135cfe

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Additional file 2: of Using in vivo oxidation status of one- and two-component redox relays to determine H2O2 levels linked to signaling and toxicity

Autor: Domènech, Alba, Ayté, José, Antunes, Fernando, Hidalgo, Elena

Figure S2. Kinetics of OxyR and Tpx1 oxidation. a In cells deficient in Tpx1 or Trx1, HA-OxyR oxidizes at low concentrations of peroxides. Aerobic or anaerobic cultures of strains AD29 (WT), AD61 (Δtrx1), and AD36 (Δtpx1) carrying an integrative st

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57d10366d0aff74f4bc7feaedc4766f9

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Additional file 3: of Using in vivo oxidation status of one- and two-component redox relays to determine H2O2 levels linked to signaling and toxicity

Autor: Domènech, Alba, Ayté, José, Antunes, Fernando, Hidalgo, Elena

Figure S3. Determination of gradients between extracellular and intracellular H2O2 concentrations. Gradients are obtained from the non-linear fitting of Eq. 1 (dashed lines) to OxyR oxidation measured experimentally (filled squares) after adding the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f74604c0e63cd6464521a227d6f7daf

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