Zobrazeno 1 - 10
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pro vyhledávání: '"Dobbie, IM"'
Autor:
Kaufmann, R, Schellenberger, P, Seiradake, E, Dobbie, IM, Jones, EY, Davis, I, Hagen, C, Grünewald, K
Publikováno v:
Nano Letters
We introduce a super-resolution technique for fluorescence cryo-microscopy based on photoswitching of standard genetically encoded fluorescent marker proteins in intact mammalian cells at low temperature (81 K). Given the limit imposed by the lack of
Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::57e61f40d703ff1034701d964ddfebb1
https://doi.org/10.1038/srep15915
https://doi.org/10.1038/srep15915
Publikováno v:
Scientific Reports
Many biological questions require fluorescence microscopy with a resolution beyond the diffraction limit of light. Super-resolution methods such as Structured Illumination Microscopy (SIM), STimulated Emission Depletion (STED) microscopy and Single M
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Autor:
Wincott M; Department of Engineering Science, University of Oxford, Oxford, OX1 3PJ, UK.; Micron Advanced Bio-imaging Unit, Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK., Jefferson A; Dementia Research Institute, Cardiff University, Cardiff, CF24 4HQ, UK, UK., Dobbie IM; Micron Advanced Bio-imaging Unit, Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK.; Department of Biology, Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA., Booth MJ; Department of Engineering Science, University of Oxford, Oxford, OX1 3PJ, UK.; Micron Advanced Bio-imaging Unit, Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK., Davis I; Micron Advanced Bio-imaging Unit, Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK.; Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK., Parton RM; Micron Advanced Bio-imaging Unit, Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK.; Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK.
Publikováno v:
Wellcome open research [Wellcome Open Res] 2022 Mar 30; Vol. 6, pp. 63. Date of Electronic Publication: 2022 Mar 30 (Print Publication: 2021).
Autor:
Boehm U; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Nelson G; Bioimaging Unit, Newcastle University, Newcastle upon Tyne, UK., Brown CM; Advanced BioImaging Facility (ABIF), McGill University, Montreal, Quebec, Canada., Bagley S; Visualisation, Irradiation & Analysis, Cancer Research UK Manchester Institute, Alderley Park, Macclesfield, UK., Bajcsy P; National Institute of Standards and Technology, Gaithersburg, MD, USA., Bischof J; Euro-BioImaging ERIC, Heidelberg, Germany., Dauphin A; Unite Génétique et Biologie du Développement U934, PICT-IBiSA, Institut Curie/Inserm/CNRS/PSL Research University, Paris, France., Dobbie IM; Department of Biochemistry, University of Oxford, Oxford, UK., Eriksson JE; Turku Bioscience Centre, Euro-Bioimaging ERIC, Turku, Finland., Faklaris O; BCM, University of Montpellier, CNRS, INSERM, Montpellier, France., Fernandez-Rodriguez J; Centre for Cellular Imaging, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden., Ferrand A; Imaging Core Facility Biozentrum, University of Basel, Basel, Switzerland., Gelman L; Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland., Gheisari A; Light Microscopy Facility, CMCB Technology Platform, TU Dresden, Dresden, Germany., Hartmann H; Light Microscopy Facility, CMCB Technology Platform, TU Dresden, Dresden, Germany., Kukat C; FACS & Imaging Core Facility, Max Planck Institute for Biology of Ageing, Cologne, Germany., Laude A; Bioimaging Unit, Newcastle University, Newcastle upon Tyne, UK., Mitkovski M; Light Microscopy Facility, Max Planck Institute of Experimental Medicine, Goettingen, Germany., Munck S; VIB BioImaging Core, Leuven, Flanders, Belgium.; VIB-KU Leuven Center for Brain and Disease, Leuven, Flanders, Belgium.; KU Leuven Department for Neuroscience, Leuven, Flanders, Belgium., North AJ; The Rockefeller University, New York, NY, USA., Rasse TM; Scientific Service Group Microscopy, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany., Resch-Genger U; Division Biophotonics, Federal Institute for Materials Research and Testing (BAM), Berlin, Germany., Schuetz LC; European Molecular Biology Laboratory, Advanced Light Microscopy Facility, Heidelberg, Germany., Seitz A; Faculty of Life Sciences, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland., Strambio-De-Castillia C; Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA., Swedlow JR; Divisions of Computational Biology and Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee, UK., Nitschke R; Life Imaging Center and Signalling Research Centres CIBSS and BIOSS, University of Freiburg, Freiburg, Germany. Roland.Nitschke@uni-freiburg.de.; BIOSS Centre for Biological Signaling Studies, Albert-Ludwigs University, Freiburg, Germany. Roland.Nitschke@uni-freiburg.de.
Publikováno v:
Nature methods [Nat Methods] 2022 Feb; Vol. 19 (2), pp. 256.