Colloidal aggregation causes inhibition of G protein-coupled receptors

Autor: Shoichet, Brian, Sassano, MF, Doak, AK, Roth, BL, Shoichet, BK

Publikováno v: Shoichet, Brian; Sassano, MF; Doak, AK; Roth, BL; & Shoichet, BK. (2013). Colloidal aggregation causes inhibition of G protein-coupled receptors. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/67v7w96d

Colloidal aggregation is the dominant mechanism for artifactual inhibition of soluble proteins, and controls against it are now widely deployed. Conversely, investigating this mechanism for membrane-bound receptors has proven difficult. Here we inves

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od_______325::41e84dc8f19b8c49217c06395569527b
http://www.escholarship.org/uc/item/67v7w96d

In Vitro and In Vivo Characterization of the Alkaloid Nuciferine.

Autor: Farrell MS; Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America., McCorvy JD; Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America., Huang XP; Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America.; National Institute of Mental Health Psychoactive Drug Screening Program, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America., Urban DJ; Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America., White KL; Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America., Giguere PM; Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America., Doak AK; Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, United States of America., Bernstein AI; Department of Environmental Health, Rollins School of Public Health and Center for Neurodegenerative Diseases, Emory University, Atlanta, Georgia, United States of America., Stout KA; Department of Environmental Health, Rollins School of Public Health and Center for Neurodegenerative Diseases, Emory University, Atlanta, Georgia, United States of America., Park SM; Departments of Psychiatry and Behavioral Sciences, Cell Biology, and Neurobiology, Mouse Behavioral and Neuroendocrine Analysis Core Facility, Duke University Medical Center, Durham, North Carolina, United States of America., Rodriguiz RM; Departments of Psychiatry and Behavioral Sciences, Cell Biology, and Neurobiology, Mouse Behavioral and Neuroendocrine Analysis Core Facility, Duke University Medical Center, Durham, North Carolina, United States of America., Gray BW; Department of Pharmacology and Toxicology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, United States of America., Hyatt WS; Department of Pharmacology and Toxicology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, United States of America., Norwood AP; Department of Pharmacology and Toxicology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, United States of America., Webster KA; Department of Psychology, Virginia Commonwealth University, Richmond, Virginia, United States of America., Gannon BM; Department of Pharmacology and Toxicology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, United States of America., Miller GW; Department of Environmental Health, Rollins School of Public Health and Center for Neurodegenerative Diseases, Emory University, Atlanta, Georgia, United States of America., Porter JH; Department of Psychology, Virginia Commonwealth University, Richmond, Virginia, United States of America., Shoichet BK; Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, United States of America., Fantegrossi WE; Department of Pharmacology and Toxicology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, United States of America., Wetsel WC; Departments of Psychiatry and Behavioral Sciences, Cell Biology, and Neurobiology, Mouse Behavioral and Neuroendocrine Analysis Core Facility, Duke University Medical Center, Durham, North Carolina, United States of America., Roth BL; Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America.; Department of Psychiatry, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America.; Program in Neuroscience, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America.; Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America.; Carolina Institute for Developmental Disabilities, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America.; Division of Chemical Biology and Medicinal Chemistry, School of Pharmacy, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America.; National Institute of Mental Health Psychoactive Drug Screening Program, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America.

Publikováno v: PloS one [PLoS One] 2016 Mar 10; Vol. 11 (3), pp. e0150602. Date of Electronic Publication: 2016 Mar 10 (Print Publication: 2016).

Colloidal aggregation and the in vitro activity of traditional Chinese medicines.

Autor: Duan D; †Department of Pharmaceutical Chemistry, University of California, San Francisco, 1700 Fourth Street, San Francisco, California 94158-2550, United States.; ‡Leslie Dan Faculty of Pharmacy, Donnelly Center, University of Toronto, 144 College Street, Toronto, Ontario M5S3M2, Canada., Doak AK; †Department of Pharmaceutical Chemistry, University of California, San Francisco, 1700 Fourth Street, San Francisco, California 94158-2550, United States., Nedyalkova L; ‡Leslie Dan Faculty of Pharmacy, Donnelly Center, University of Toronto, 144 College Street, Toronto, Ontario M5S3M2, Canada., Shoichet BK; †Department of Pharmaceutical Chemistry, University of California, San Francisco, 1700 Fourth Street, San Francisco, California 94158-2550, United States.; ‡Leslie Dan Faculty of Pharmacy, Donnelly Center, University of Toronto, 144 College Street, Toronto, Ontario M5S3M2, Canada.

Publikováno v: ACS chemical biology [ACS Chem Biol] 2015 Apr 17; Vol. 10 (4), pp. 978-88. Date of Electronic Publication: 2015 Feb 09.

Colloidal drug formulations can explain "bell-shaped" concentration-response curves.

Autor: Owen SC; Donnelly Centre, Department of Chemical Engineering & Applied Chemistry, Institute of Biomaterials & Biomedical Engineering, Department of Chemistry, University of Toronto , 160 College Street, Toronto, Ontario M5S3E1, Canada., Doak AK, Ganesh AN, Nedyalkova L, McLaughlin CK, Shoichet BK, Shoichet MS

Publikováno v: ACS chemical biology [ACS Chem Biol] 2014 Mar 21; Vol. 9 (3), pp. 777-84. Date of Electronic Publication: 2014 Jan 15.

Colloidal aggregation causes inhibition of G protein-coupled receptors.

Autor: Sassano MF; Department of Pharmacology and the NIMH Psychoactive Drug Screening Program, University of North Carolina Chapel Hill School of Medicine , Chapel Hill, North Carolina 27759, USA., Doak AK, Roth BL, Shoichet BK

Publikováno v: Journal of medicinal chemistry [J Med Chem] 2013 Mar 28; Vol. 56 (6), pp. 2406-14. Date of Electronic Publication: 2013 Mar 12.