Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Dmitry V. Sarkisov"'
Publikováno v:
Journal of Biological Chemistry. 282:25517-25526
Biological messengers can be "caged" by adding a single photosensitive group that can be photolyzed by a light flash to achieve spatially and temporally precise biochemical control. Here we report that photolysis of a double-caged form of the second
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbf90c01acfb02acf549a0f936528e5d
https://doi.org/10.1074/jbc.m609672200
https://doi.org/10.1074/jbc.m609672200
Publikováno v:
Nature Methods. 2:837-843
Light-sensitive 'caged' molecules provide a means of rapidly and noninvasively manipulating biochemical signals with submicron spatial resolution. Here we describe a new optical system for rapid uncaging in arbitrary patterns to emulate complex neura
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8a621fc4f9ed21542ea8e0bce23df31
https://doi.org/10.1038/nmeth793
https://doi.org/10.1038/nmeth793
Publikováno v:
Journal of Neurophysiology
In vivo two-photon calcium imaging provides the opportunity to monitor activity in multiple components of neural circuitry at once. Here we report the use of bulk-loading of fluorescent calcium indicators to record from axons, dendrites, and neuronal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a967e3e3362264a3fcc5c333e0c7e81
https://doi.org/10.1152/jn.01013.2004
https://doi.org/10.1152/jn.01013.2004
Publikováno v:
Cold Spring Harbor protocols. 2012(8)
The method of patterned photoactivation is a natural fit for the study of neuronal dendritic integration. Photoactivatable molecules that influence a wide range of extracellular and intracellular neurophysiological functions are available. The choice
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec12ff92e9244eed23ad888cd14a9529
https://pubmed.ncbi.nlm.nih.gov/22854575
https://pubmed.ncbi.nlm.nih.gov/22854575
Publikováno v:
Cold Spring Harbor protocols. 2012(8)
The method of patterned photoactivation is a natural fit for the study of neuronal dendritic integration. Photoactivatable molecules that influence a wide range of extracellular and intracellular neurophysiological functions are available. The choice
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb2b8008a98ad57f8fa434ea0c5bccbe
https://pubmed.ncbi.nlm.nih.gov/22854574
https://pubmed.ncbi.nlm.nih.gov/22854574
Autor:
Dmitry V. Sarkisov, Samuel S.-H. Wang
Associative long-term depression (LTD) at cerebellar parallel fiber–Purkinje cell synapses is sensitive to the temporal order in which the parallel fiber is coactivated with the climbing fiber input, but how order sensitivity is achieved is unknown
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ad1184688ebc7a74e15110595aa67da
https://europepmc.org/articles/PMC6671165/
https://europepmc.org/articles/PMC6671165/
Autor:
Dmitry V. Sarkisov, Samuel S.-H. Wang
Publikováno v:
Nature protocols. 1(2)
Photolysis of caged compounds is a powerful tool for studying subcellular physiological functions. Here we describe protocols for the alignment and calibration of a focal uncaging system. We also report procedures for convenient quantitative calibrat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d043b52967383b7e239cdc1e2f0e9fa
https://pubmed.ncbi.nlm.nih.gov/17406314
https://pubmed.ncbi.nlm.nih.gov/17406314
Autor:
Samuel S.-H. Wang, Dmitry V. Sarkisov
Publikováno v:
Neuromethods ISBN: 9781588297051
Patch-clamp recording is a powerful approach to monitoring membrane electrical activity with high temporal resolution. However, the spatial resolution of patch-clamp recording in a distributed structure such as a neuron or a brain slice is limited by
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7a7a533dcaffe3e2257513583bfca805
https://doi.org/10.1007/978-1-59745-492-6_5
https://doi.org/10.1007/978-1-59745-492-6_5
Publikováno v:
Biochemistry. 43(42)
Conventional kinesin is a multifunctional motor protein that transports numerous organelles along microtubules. The specificity of kinesin-cargo binding is thought to depend on the type(s) of light chains that a kinesin molecule contains. We have sho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f714415573ff8d2b99702b2b8a6fbe58
https://pubmed.ncbi.nlm.nih.gov/15491159
https://pubmed.ncbi.nlm.nih.gov/15491159
Publikováno v:
Cold Spring Harbor Protocols. 2012:pdb.top070631
The method of patterned photoactivation is a natural fit for the study of neuronal dendritic integration. Photoactivatable molecules that influence a wide range of extracellular and intracellular neurophysiological functions are available. The choice
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7ca2dbda9477e32544c888c877e122ec
https://doi.org/10.1101/pdb.top070631
https://doi.org/10.1101/pdb.top070631