Tryptophan and Non-Tryptophan Fluorescence of the Eye Lens Proteins Provides Diagnostics of Cataract at the Molecular Level

Autor: Nicola M. Howarth, Rory R. Duncan, Kim K. Buttenschön, Dmitry M. Gakamsky, Daniel J. Daly, Anna Gakamsky, Baljean Dhillon

Publikováno v: Gakamsky, A, Duncan, R R, Howarth, N M, Dhillon, B, Buttenschön, K K, Daly, D J & Gakamsky, D 2017, ' Tryptophan and Non-Tryptophan Fluorescence of the Eye Lens Proteins Provides Diagnostics of Cataract at the Molecular Level ', Scientific Reports, vol. 7, pp. 40375 . https://doi.org/10.1038/srep40375
Scientific Reports

The chemical nature of the non-tryptophan (non-Trp) fluorescence of porcine and human eye lens proteins was identified by Mass Spectrometry (MS) and Fluorescence Steady-State and Lifetime spectroscopy as post-translational modifications (PTM) of Trp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6a8d751190f0b659d2ae6b7d4701af4
https://hdl.handle.net/20.500.11820/5b9966c7-04fc-4189-b96c-f9f790cc7cdd

T cell receptor-ligand interactions: A conformational preequilibrium or an induced fit

Autor: Dmitry M. Gakamsky, Immanuel F. Luescher, Israel Pecht

Publikováno v: Proceedings of the National Academy of Sciences. 101:9063-9066

Kinetic parameters of T cell receptor (TCR) interactions with its ligand have been proposed to control T cell activation. Analysis of kinetic data obtained has so far produced conflicting insights; here, we offer a consideration of this problem. As a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b5708647528a25ce9c47aa3f0cf4ded
https://doi.org/10.1073/pnas.0402840101

Efficient induction of cytotoxic CD8+ T cells against exogenous proteins: establishment and characterization of a T cell line specific for the membrane protein ActA ofListeria monocytogenes

Autor: Ayub Darji, Jürgen Wehland, Trinad Chakraborty, Dmitry M. Gakamsky, Dunja Bruder, Israel Pecht, Siegfried Weiss

Publikováno v: European Journal of Immunology. 28:2630-2639

The property of listeriolysin (LLO) to introduce soluble passenger proteins into the cytosol of antigen-presenting cells allows the induction of CD8+ cytotoxic T cells against such antigens. To overcome the potential problem of presentation of the im

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fab951e805def65d5b2325dd4f56176f
https://doi.org/10.1002/(sici)1521-4141(199809)28:09<2630::aid-immu2630>3.0.co

Selective steady-state and time-resolved fluorescence spectroscopy of an HLA-A2-peptide complex

Autor: Israel Pecht, Jack L. Strominger, Elisha Haas, Penelope Robbins, Dmitry M. Gakamsky

Publikováno v: Immunology Letters. 44:195-201

The human class I major histocompatibility complex (MHC) encoded molecule HLA-A2 loaded with the high-affinity peptide GILGRVFTL (p790), was studied by means of steady-state and picosecond fluorescence intensity and fluorescence anisotropy methods. T

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21285c983f7916381d899c9b8f1f3acc
https://doi.org/10.1016/0165-2478(94)00214-c

Picosecond fluorescence spectroscopy of a single-chain class I major histocompatibility complex encoded protein in its peptide loaded and unloaded states

Autor: Israel Pecht, Philippe Kourilsky, David M. Ojcius, Elisha Haas, Dmitry M. Gakamsky, Kerstin Dittes, Gilad Haran

Publikováno v: Immunology Letters. 40:125-132

The tryptophan fluorescence properties of two different peptide complexes of the single-chain H-2K d (SC-K d ) were studied by means of the single-photon counting technique. The latter enables time-resolved measurements of fluorescence intensity and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fee4bba5e141610d328a01758781727b
https://doi.org/10.1016/0165-2478(94)90183-x