Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Dmitry A. Tsybulsky"'
Autor:
Vadim V. Shmanai, D. L. Yakovlev, V. I. Stepuro, Dmitry A. Tsybulsky, V. A. Povedailo, B. V. Ronishenko
Publikováno v:
Journal of Applied Spectroscopy. 85:605-610
Fluorescence quenching of four dyes [acridine orange (AO), rhodamine B (Rd B), rhodamine 110 (Rd 110), and rhodamine 640 (Rd 640)] by colloidal graphene oxide (GO) in phosphate buffer (pH 6.0) was studied. Dynamic and static quenching were analyzed u
Autor:
Fan Fan, Sergei A. Tikhomirov, Pham Van Duong, Aliaksandr S Kruhlik, Vadim V. Shmanai, V. A. Povedailo, Dmitrii L Yakovlev, Ivan L. Lysenko, O. L. Sharko, Pham Thi Hong Minh, Dmitry A. Tsybulsky, Yury V Martynenko-Makaev
Publikováno v:
Journal of fluorescence. 30(3)
Bright fluorescent probes with enhanced intensities in the fluorescein channel are of great value for plenty of biological applications. To design effective probes one should introduce as many as possible fluorophores to the biomolecule while leaving
Publikováno v:
Journal of Applied Spectroscopy. 84:452-459
Dynamic and static quenching of 6-(2′,7′-dimethoxy-4′,5′-dichloro)carboxyfluorescein (JOE) by nucleosides (deoxyadenosine, deoxycytidine, deoxyguanosine, thymidine, and deoxyuridine) in Tris-acetate buffer solution was analyzed using the Ster
Autor:
Yury V. Martynenko, Vadim V. Shmanai, Maksim V. Kvach, Timofei S. Zatsepin, Sergey K. Zavriev, Andrey A. Formanovsky, Alexander A. Stakheev, Ilya O. Aparin, Sergey V. Gontarev, Dmitry Y. Ryazantsev, Igor A. Prokhorenko, Dmitry A. Tsybulsky, Vladimir A. Korshun
Publikováno v:
Molecular and Cellular Probes. 30:285-290
Molecular beacons carrying JOE dye (4',5'-dichloro-2',7'-dimethoxy-6-carboxyfluorescein) on a rigid or flexible linker and one or two BHQ1 quenchers have been prepared and tested in real-time PCR using Fusarium avenaceum elongation factor 1α DNA tem
Autor:
Maksim V. Kvach, Vladimir A. Korshun, Vadim V. Shmanai, Irina A. Stepanova, Dmitry A. Tsybulsky
Publikováno v:
The Journal of Organic Chemistry. 77:977-984
A convenient procedure for the preparation of the fluorescent dye 4',5'-dichloro-2',7'-dimethoxy-5(6)-carboxyfluorescein (JOE) is reported; the overall yield achieved starting from isovanillin is 10 times higher (40% vs 4%) compared to the known proc
Autor:
Vladimir A. Korshun, Dmitry A. Tsybulsky, Irina A. Stepanova, S. L. Bondarev, Alexey V. Ustinov, Sergey V. Gontarev, Vadim V. Shmanai, Maksim V. Kvach
Publikováno v:
Bioconjugate Chemistry. 18:1691-1696
Pentafluorophenyl esters of 5- and 6-carboxyfluorescein-3',6'-O-dipivalate can be easily separated in multigram quantities by column chromatography. The individual isomers were converted into stable phosphoramidites suitable for oligonucleotide synth
Autor:
Irina A. Stepanova, Igor A. Prokhorenko, Yury V. Martynenko, Vladimir A. Korshun, Dmitry Y. Ryazantsev, Sergey K. Zavriev, Sergey V. Gontarev, Vadim V. Shmanai, Maksim V. Kvach, Dmitry A. Tsybulsky
Publikováno v:
The Analyst. 139(11)
Convenient preparation of fluorogenic hairpin DNA probes (molecular beacons) carrying a pair of FAM fluorophores (located close to 5′-terminus of the probe) or a pair of BHQ1 quenchers on 3′-terminus (with (BHQ1)2 or BHQ1–BHQ1 composition) is r
Autor:
Dmitry A. Tsybulsky, Igor A. Prokhorenko, Irina A. Stepanova, Maksim V. Kvach, Vladimir A. Korshun, Vadim V. Shmanai
Publikováno v:
Current Protocols in Nucleic Acid Chemistry
This unit describes the preparation of 5- and 6-carboxy derivatives of the xanthene fluorescent dyes fluorescein (FAM), 4',5'-dichloro-2',7'-dimethoxy-fluorescein (JOE), and tetramethylrhodamine (TAMRA) as individual isomers, and their conversion to
Autor:
Sergey K. Zavriev, Maksim V. Kvach, Igor A. Prokhorenko, Yury V. Martynenko, Pavel M. Philipchenko, Vladimir A. Korshun, Dmitry Y. Ryazantsev, Dmitry A. Tsybulsky, Vadim V. Shmanai
Publikováno v:
Analytical and bioanalytical chemistry. 404(1)
A typical TaqMan™ real-time PCR probe contains a 5′-fluorescent dye and a 3′-quencher. In the course of the amplification, the probe is degraded starting from the 5′-end, thus releasing fluorescent dye. Some fluorophores (including fluorescei
Autor:
Maksim V. Kvach, Dmitry A. Tsybulsky, Alexey V. Ustinov, Irina A. Stepanova, Stanislav L. Bondarev, Sergey V. Gontarev, Vladimir A. Korshun, Vadim V. Shmanai
Publikováno v:
Bioconjugate Chemistry; Sep2007, Vol. 18 Issue 5, p1691-1696, 6p