Výsledky vyhledávání - "Dmitrii M Bubnov"

Akademický článek

A study on L-threonine and L-serine uptake in Escherichia coli K-12

Autor: Andrey A. Khozov, Dmitrii M. Bubnov, Eugeny D. Plisov, Tatiana V. Vybornaya, Tigran V. Yuzbashev, Gennaro Agrimi, Eugenia Messina, Agnessa A. Stepanova, Maxim D. Kudina, Natalia V. Alekseeva, Alexander I. Netrusov, Sergey P. Sineoky

Publikováno v: Frontiers in Microbiology, Vol 14 (2023)

In the current study, we report the identification and characterization of the yifK gene product as a novel amino acid carrier in E. coli K-12 cells. Both phenotypic and biochemical analyses showed that YifK acts as a permease specific to L-threonine

Externí odkaz: https://doaj.org/article/5ea62c6237b3401d8eb7038e7f5f253a

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Robust counterselection and advanced λRed recombineering enable markerless chromosomal integration of large heterologous constructs

Autor: Dmitrii M Bubnov, Tigran V Yuzbashev, Andrey A Khozov, Olga E Melkina, Tatiana V Vybornaya, Guy-Bart Stan, Sergey P Sineoky

Publikováno v: Nucleic acids research. 50(15)

Despite advances in bacterial genome engineering, delivery of large synthetic constructs remains challenging in practice. In this study, we propose a straightforward and robust approach for the markerless integration of DNA fragments encoding whole m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a60a19904d02e57079a460b667d5353
https://pubmed.ncbi.nlm.nih.gov/35920321

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Excision of selectable markers from the Escherichia coli genome without counterselection using an optimized λRed recombineering procedure

Autor: Alexander I. Netrusov, Dmitrii M. Bubnov, S. P. Sineoky, Tatiana V. Vybornaya, Tigran V. Yuzbashev

Publikováno v: Journal of microbiological methods. 158

The introduction of chromosomal mutations into the E. coli genome using λRed-mediated recombineering includes two consecutive steps—the insertion of an antibiotic resistance gene and the subsequent excision of the marker. The second step usually r

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bf0ba41d463e2000168b4a00c55e354
https://pubmed.ncbi.nlm.nih.gov/30738107

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Development of new versatile plasmid-based systems for λRed-mediated Escherichia coli genome engineering

Autor: Dmitrii M. Bubnov, S. P. Sineoky, Alexander I. Netrusov, Tatiana V. Vybornaya, Tigran V. Yuzbashev

Publikováno v: Journal of microbiological methods. 151

Plasmid-based systems are the most appropriate for multistep lambda Red (λRed)-mediated recombineering, such as the assembly of strains for biotechnological applications. Currently, the widely used λRed-expressing plasmids use a temperature-sensiti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e34c84bd3b70b8503f0d8005211d34d2
https://pubmed.ncbi.nlm.nih.gov/29885886

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