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A comparative analysis of the efficiency of bird and mammalian antibodies in HBsAg sandwich assay

Autor: Segal Ol, D. A. Dmitriev, Dmitriev Ad, Yu. S. Massino, V. F. Lavrov, Yu. N. Tarakanova, A. A. Pechelyulko

Publikováno v: Applied Biochemistry and Microbiology. 53:114-122

The antigen-binding capacities of chicken and goat polyclonal antibodies and mouse monoclonal antibodies specific for the hepatitis B virus surface antigen were compared via enzyme immunoassay. It was shown that the use of both adsorbed and detecting

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5a6761a4adddf293f645501554c7ffcb
https://doi.org/10.1134/s0003683817010136

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ANALYSIS OF EFFECTIVENESS OF USING IgY FROM CHICKEN IN SANDWICH METHOD OF HBsAg TESTING

Autor: Yu. N. Tarakanova, Dmitriev Ad, A. A. Pechelyulko, Segal Ol, V. F. Lavrov, Yu. S. Massino, D. A. Dmitriev

Publikováno v: Журнал микробиологии, эпидемиологии и иммунобиологии, Vol 0, Iss 3, Pp 44-51 (2016)

Aim. Study antigen-binding ability of polyclonal antibodies (PCA) of chicken compared with monoclonal antibodies (MCA) of mice in the model of interaction with HBsAg. Materials and methods. Mice MCA 18C8 and MKA F3/F4 (IgG) were used, effective in en

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::08536c9cbd46839ad7c6521196a55ffa
https://microbiol.elpub.ru/jour/article/view/48

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Effect of conditions of monoclonal antibody adsorption on antigen-binding activity

Autor: O. V. Fartushnaya, D. A. Yakovleva, Yu. N. Tarakanova, Yu. S. Massino, G. I. Kolyaskina, Segal Ol, M. D. Smirnova, Dmitriev Ad, V. F. Lavrov, D. A. Dmitriev

Publikováno v: Applied Biochemistry and Microbiology. 48:506-512

The dependence of the antigen-binding activity of immobilized antibodies on pH of a saturating buffer has been investigated. We analyzed 28 monoclonal antibodies (MCAs) produced by various hybridomas to three virus antigens, i.e., the nuclear p23 pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::21a0d463a380aa5e4a1ee8d0cb78d4eb
https://doi.org/10.1134/s0003683812050122

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Western blot analysis of human and rat serotonin transporter in platelets and brain using site-specific antibodies: Evidence that transporter undergoes endoproteolytic cleavage

Autor: Magnolia I. Factor, M. D. Smirnova, Yulia S Massino, Dinora A. Yakovleva, G. I. Kolyaskina, Segal Ol, Dmitriev Ad, Kizim Ea, Pavlova Ev, Brusov Os, Dmitriy A. Dmitriev

Publikováno v: Clinica Chimica Acta. 356:76-94

Background Serotonin transporter (SERT) is important target molecule for many antidepressive drugs and substances of abuse and is implicated in psychiatric disorders. We performed immunoblotting analysis of human and rat SERT in platelets and brain u

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae6d85eff8fa581473c9c4e41089e48c
https://doi.org/10.1016/j.cccn.2004.12.019

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Analysis of Serotonin Transporter in Human Platelets by Immunoblotting Using Site-Specific Antibodies

Autor: M. D. Smirnova, Pavlova Ev, I V Dobrohotov, D E Hwun, Brusov Os, Dmitriev Ad, Dinora A. Yakovleva, Yu. S. Massino, G. I. Kolyaskina, Segal Ol, M I Factor

Publikováno v: Biochemistry (Moscow). 69:629-641

We have produced a panel of site-specific antibodies recognizing different regions of the human serotonin transporter (SERT). This panel included: 1) monoclonal antibodies 23C5 (mAbs 23C5) to the C-terminal region (amino acid residues 597-630); 2) po

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::379ec2e049e4c51e8b26c9a638e2e086
https://doi.org/10.1023/b:biry.0000033736.50015.be

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Analysis of the binding of bispecific monoclonal antibodies with immobilized antigens (human IgG and horseradish peroxidase) using a resonant mirror biosensor

Autor: M. D. Smirnova, Yulia S Massino, Dmitriev Ad, Pavlova Ev, D. A. Dmitriev, Alexander I. Archakov, Yuriy D. Ivanov, Oksana Gnedenko, Segal Ol, Konstantin G. Gurevich, G. I. Kolyaskina, Alexander P. Osipov, Alexey M. Egorov

Publikováno v: Journal of Immunological Methods. 261:103-118

The interaction between two monoclonal antibodies (mAbs) and their corresponding bispecific antibody (bAb) with immobilized antigens has been examined using a resonant mirror biosensor (IAsys). BAbs were produced by cell fusion. The analysed panel of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84bfe7d46fdb1eb3950a7a4a4965e4d1
https://doi.org/10.1016/s0022-1759(01)00558-0

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[Untitled]

Autor: Konstantin G. Gurevich, G. I. Kolyaskina, Alexander P. Osipov, Yuri D. Ivanov, Dmitriev Ad, Alexander I. Archakov, Pavlova Ev, Alexey M. Egorov, Oksana Gnedenko, D. A. Dmitriev, Segal Ol, M. D. Smirnova, Yulia S Massino

Publikováno v: Biochemistry (Moscow). 67:1356-1365

The interaction between two different monoclonal antibodies (Mabs) and their corresponding bispecific antibodies (Babs) with immobilized antigens was investigated using an optical biosensor (IAsys). The analyzed panel of affinity-purified antibodies

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7986c67080a4d9af4850ff831bb9b42d
https://doi.org/10.1023/a:1021805909314

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The comparison of the ability of monoclonal antibodies directed to different proteins (human IgG, human myoglobin and HRP) and bispecific antibodies derived thereof to bind antigens immobilized on a surface of a solid phase

Autor: G. I. Kolyaskina, Pavlova Ev, Alexander P. Osipov, Alexey M. Egorov, M. D. Smirnova, Yulia S Massino, Segal Ol, Dmitriev Ad, D. A. Dmitriev

Publikováno v: Clinica Chimica Acta. 309:57-71

Background: Bindings of mouse monoclonal antibodies (mAbs) and affinity purified bispecific antibodies (bAbs), derived thereof, to antigens adsorbed on immunoplates have been compared, using ELISA and RIA methods. Methods: The analysed panel of antib

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f41cf7ef4c154cc1d5790931e09e9143
https://doi.org/10.1016/s0009-8981(01)00531-9

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[Untitled]

Autor: Pavlova Ev, Dmitriev Ad, Yu. S. Massino, G. I. Kolyaskina, Segal Ol, Alexey M. Egorov, Alexander P. Osipov, D. A. Dmitriev, M. D. Smirnova

Publikováno v: Russian Journal of Bioorganic Chemistry. 27:232-240

The ability of bispecific antibodies (Babs) formed by fusion of hybridomas and parent monoclonal antibodies (Mabs) to interact with the solid phase-adsorbed antigens was studied. Mabs specific to the three different antigens [horseradish peroxidase (

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4164a47200aa59a414f6daa8343b30ca
https://doi.org/10.1023/a:1011352419668

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