PICALM Rescues Endocytic Defects Caused by the Alzheimer’s Disease Risk Factor APOE4

Autor: Priyanka Narayan, Grzegorz Sienski, Julia M. Bonner, Yuan-Ta Lin, Jinsoo Seo, Valeriya Baru, Aftabul Haque, Blerta Milo, Leyla A. Akay, Agnese Graziosi, Yelena Freyzon, Dirk Landgraf, William R. Hesse, Julie Valastyan, M. Inmaculada Barrasa, Li-Huei Tsai, Susan Lindquist

Publikováno v: Cell Reports, Vol 33, Iss 1, Pp 108224- (2020)

Summary: The ε4 allele of apolipoprotein E (APOE4) is a genetic risk factor for many diseases, including late-onset Alzheimer’s disease (AD). We investigate the cellular consequences of APOE4 in human iPSC-derived astrocytes, observing an endocyti

Externí odkaz: https://doaj.org/article/371d6e17de3e4dcca3170e73f880da3a

Scarless Gene Tagging with One-Step Transformation and Two-Step Selection in Saccharomyces cerevisiae and Schizosaccharomyces pombe.

Autor: Dirk Landgraf, Dann Huh, Erinc Hallacli, Susan Lindquist

Publikováno v: PLoS ONE, Vol 11, Iss 10, p e0163950 (2016)

Gene tagging with fluorescent proteins is commonly applied to investigate the localization and dynamics of proteins in their cellular environment. Ideally, a fluorescent tag is genetically inserted at the endogenous locus at the N- or C- terminus of

Externí odkaz: https://doaj.org/article/e81fd0af30f94994b1de4a4ac16b59cd

Correction: Corrigendum: Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells

Autor: Burak Okumus, Dirk Landgraf, Ghee Chuan Lai, Somenath Bakshi, Juan Carlos Arias-Castro, Sadik Yildiz, Dann Huh, Raul Fernandez-Lopez, Celeste N. Peterson, Erdal Toprak, Meriem El Karoui, Johan Paulsson

Publikováno v: Nature Communications, Vol 7, Iss 1, Pp 1-1 (2016)

Nature Communications 7: Article number: 11641 (2016); Published: 18 May 2016; Updated: 24 June 2016. The original version of this Article contained an error in the spelling of the author Somenath Bakshi, which was incorrectly given as Somenath Bakhs

Externí odkaz: https://doaj.org/article/3d19b3099066482eb2eeb3bf6c834073

Quantification of very low-abundant proteins in bacteria using the HaloTag and epi-fluorescence microscopy

Autor: Hannah Taylor, Alessia Lepore, Sebastián Jaramillo-Riveri, Dirk Landgraf, Burak Okumus, Lorna McLaren, Somenath Bakshi, Johan Paulsson, Meriem El Karoui

Publikováno v: Scientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
Lepore, A, Taylor, H, Landgraf, D, Okumus, B, Jaramillo-Riveri, S, McLaren, L, Bakshi, S, Johan, P & El Karoui, M 2019, ' Quantification of very low-abundant proteins in bacteria using the HaloTag and epi-fluorescence microscopy ', Scientific Reports, vol. 9, 7902 . https://doi.org/10.1038/s41598-019-44278-0
Scientific Reports

Cell biology is increasingly dependent on quantitative methods resulting in the need for microscopic labelling technologies that are highly sensitive and specific. Whilst the use of fluorescent proteins has led to major advances, they also suffer fro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e122958588b55a0d8c5fadd5cc32e67
http://link.springer.com/article/10.1038/s41598-019-44278-0

Fast-Growing Trees Species—Opportunities and Risks for Sustainable Agricultural and Forest Land Use Systems

Autor: Dirk Landgraf

Publikováno v: Forests. 13:829

The cultivation of fast-growing tree species has noticeably increased worldwide in recent years [...]

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::692f4989d44b3f9e9d8cfd86f1f20455
https://doi.org/10.3390/f13060829