Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Dirk Eberhard"'
Autor:
K. Tessmer, Petra Jakob, William F. Mueller, A. M. Michon, Frederik Ziebell, Nico Zinn, Arne H. Smits, Paola Grandi, Marcus Bantscheff, Dirk Eberhard, Tilmann Bürckstümmer, Han Sun, Lars M. Steinmetz, M. Fälth Savitski, Wolfgang Huber, Gerard Drewes, Sandra Clauder-Münster, Gerard Joberty
Gene knockouts (KOs) are efficiently engineered through CRISPR-Cas9-induced frameshift mutations. While DNA editing efficiency is readily verified by DNA sequencing, a systematic understanding of the efficiency of protein elimination has been lacking
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::951931493cf18c69c107ad31025f5022
Autor:
Rab K. Prinjha, Anne Rueger, Neil Stuart Garton, Dirk Eberhard, David Matthew Wilson, Marcus Bantscheff, Robert J. Sheppard, Kevin Lee, Jack A. Brown, Nigel Ramsden, Susan Marie Westaway, Gerard Drewes, Philip G. Humphreys, Toby Mathieson, Gerard Joberty, Marcel Muelbaier, Valerie Reader, Markus Boesche
Publikováno v:
ACS Chemical Biology. 11:2002-2010
The 2-oxoglutarate-dependent dioxygenase target class comprises around 60 enzymes including several subfamilies with relevance to human disease, such as the prolyl hydroxylases and the Jumonji-type lysine demethylases. Current drug discovery approach
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1e514fee0ea43db13a3bff9292a026a
https://doi.org/10.1021/acschembio.6b00080
https://doi.org/10.1021/acschembio.6b00080
Autor:
Maria Fälth Savitski, William F. Mueller, Gerard Joberty, Frederik Ziebell, Marcus Bantscheff, Karen Tessmer, Lars M. Steinmetz, Tilmann Bürckstümmer, Paola Grandi, Anne-Marie Michon, Gerard Drewes, Petra Jakob, Dirk Eberhard, Arne H. Smits, Hanice Sun, Sandra Clauder-Münster, Wolfgang Huber, Nico Zinn
Publikováno v:
Nature methods. 16(11)
Gene knock outs (KOs) are efficiently engineered through CRISPR-Cas9-induced frameshift mutations. While the efficiency of DNA editing is readily verified by DNA sequencing, a systematic understanding of the efficiency of protein elimination has been
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63a2d508003a9c6c40b0793ddfe66904
https://pubmed.ncbi.nlm.nih.gov/31659326
https://pubmed.ncbi.nlm.nih.gov/31659326
Autor:
Holger Franken, Dirk Eberhard, Marcus Bantscheff, Mikhail M. Savitski, Friedrich B M Reinhard, Wolfgang Huber, Dorothee Childs, Thilo Werner, Maria Fälth Savitski, Carola Doce, Gerard Drewes
Publikováno v:
Nature Methods. 12:1129-1131
We extended thermal proteome profiling to detect transmembrane protein-small molecule interactions in cultured human cells. When we assessed the effects of detergents on ATP-binding profiles, we observed shifts in denaturation temperature for ATP-bin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fcb9a1dc0e49393e87bdfe376c868c9c
https://doi.org/10.1038/nmeth.3652
https://doi.org/10.1038/nmeth.3652
Autor:
Robert P. Davis, Kelly Locke, Rab K. Prinjha, Peter D. Craggs, Gang Yao, Chun-wa Chung, Kenneth E Lind, Oxana Polyakova, Jim E Coote, Daniel J. Slade, Jim Thorpe, John Liddle, David Matthew Wilson, Benjamin D. Bax, Denisa D. Wagner, Kimberly Martinod, Ryan P. Bingham, Yu Hua Chen, Kevin L. Bicker, Claire Maller, Matthew Campbell, Gerard Joberty, Huw D. Lewis, Dirk Eberhard, Martin Rüdiger, Gerard Drewes, Paul R. Thompson, Cecil E Rise, Robert J. Sheppard, Chris Patten, Stephen John Atkinson, Michael David Barker, Pamela Thomas
Publikováno v:
Nature chemical biology
PAD4 has been strongly implicated in the pathogenesis of autoimmune, cardiovascular and oncological diseases through clinical genetics and gene disruption in mice. New selective PAD4 inhibitors binding a calcium-deficient form of the PAD4 enzyme have
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::edf0de94c1b2987c42df9e5a113919ce
https://doi.org/10.1038/nchembio.1735
https://doi.org/10.1038/nchembio.1735
Autor:
Catherine Swales, Angela Bridges, Chun-wa Chung, Kevin Lee, Marcus Bantscheff, Dirk Eberhard, Julia Smith, John Liddle, Paul Rowland, C. Bountra, Robert J. Sheppard, Palwinder K. Mander, Emma J. Jones, Dinshaw J. Patel, Sue Hutchinson, Gerard Drewes, J. Mosley, Cesar Ramirez-Molina, Pamela Thomas, Laurens Kruidenier, Roy Katso, K.H. Che, Zhongjun Cheng, Christopher J. Schofield, Gerard Joberty, Hawa Diallo, Melanie Leveridge, Anthony Tumber, Robert Tanner, Udo Oppermann, David Matthew Wilson
Publikováno v:
Nature, vol 488, iss 7411
The jumonji (JMJ) family of histone demethylases are Fe2+- and α-ketoglutarate-dependent oxygenases that are essential components of regulatory transcriptional chromatin complexes. These enzymes demethylate lysine residues in histones in a methylati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e039405fd27845021f2d7f05d369eb4d
https://doi.org/10.1038/nature11262
https://doi.org/10.1038/nature11262
Autor:
Gerard Drewes, Sonja Ghidelli, Thilo Werner, Dirk Eberhard, Ulrich Kruse, Marcus Bantscheff, L. L. Frenzel, C. M. Wendtner, Christian P. Pallasch, S. K. Maier
Publikováno v:
Leukemia. 25:89-100
The pharmacological induction of apoptosis in neoplastic B cells presents a promising therapeutic avenue for the treatment of chronic lymphocytic leukemia (CLL). We profiled a panel of clinical multi-kinase inhibitors for their ability to induce apop
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a68b241e4bf4d0be48a6175a29d55d12
https://doi.org/10.1038/leu.2010.233
https://doi.org/10.1038/leu.2010.233
Autor:
Gerard Drewes, Andreas Bauer, Toby Mathieson, Bernhard Kuster, Gitte Neubauer, Yann Abraham, Marcus Bantscheff, Carsten Hopf, Manfred Raida, Scott Hobson, Gavain Sweetman, Ulrich Kruse, Jens Rick, Nigel Ramsden, Tewis Bouwmeester, Sonja Bastuck, Valerie Reader, Dirk Eberhard, Jessica Perrin, Markus Boesche, Christina Rau
Publikováno v:
Nature Biotechnology. 25:1035-1044
We describe a chemical proteomics approach to profile the interaction of small molecules with hundreds of endogenously expressed protein kinases and purine-binding proteins. This subproteome is captured by immobilized nonselective kinase inhibitors (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f04550d4646c3a3d6c190b8e33a18b6
https://doi.org/10.1038/nbt1328
https://doi.org/10.1038/nbt1328
Autor:
Renate Hiesgen, Emil Roduner, K. Andreas Friedrich, Elena Aleksandrova, Till Kaz, Dirk Eberhard
Publikováno v:
ChemPhysChem. 8:519-522
The present work demonstrates that EC-AFM is a very useful new tool for identification and spatially resolved characterization of proton conductivity at the membrane surface in comparison with topography, however it does not provide insight into the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58089db64fc6ac3aaa1352e410fdccbb
https://doi.org/10.1002/cphc.200600704
https://doi.org/10.1002/cphc.200600704
Autor:
Narges Aghaallaei, Baubak Bajoghli, Christina Murko, Anja Huber, Antonio Simeone, Ronald Stebegg, Dirk Eberhard, Maria Fink, Thomas Czerny, Thomas Heimbucher
Publikováno v:
Molecular and Cellular Biology. 27:340-351
One of the earliest organizational decisions in the development of the vertebrate brain is the division of the neural plate into Otx2-positive anterior and Gbx2-positive posterior territories. At the junction of these two expression domains, a local
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac83d7493066a70eac342dbb243d491d
https://doi.org/10.1128/mcb.00811-06
https://doi.org/10.1128/mcb.00811-06