Zobrazeno 1 - 10
of 317
pro vyhledávání: '"Direct RNA sequencing"'
Publikováno v:
RNA Biology, Vol 21, Iss 1, Pp 1-15 (2024)
ABSTRACT2´-O-methylation (Nm) is one of the most abundant modifications found in both mRNAs and noncoding RNAs. It contributes to many biological processes, such as the normal functioning of tRNA, the protection of mRNA against degradation by the d
Externí odkaz:
https://doaj.org/article/b4116d68eda54be283e2902fcc05a789
Autor:
Jundong Rong, Yushan Zheng, Zeyu Zhang, Jun Zhang, Yuying Gu, Tian Hua, Mengna Zhao, Lili Fan, Zhiwen Deng, Yanmei Pan, Bingjun Li, Liguang Chen, Tianyou He, Lingyan Chen, Jing Ye, Hangxiao Zhang, Lianfeng Gu
Publikováno v:
Advanced Science, Vol 11, Iss 40, Pp n/a-n/a (2024)
Abstract Fokienia hodginsii (F. hodginsii), belonging to the genus Fokienia of the Cupressaceae. F. hodginsii has significant application value due to its wood properties and great research value in evolutionary studies as a gymnosperm. However, the
Externí odkaz:
https://doaj.org/article/dcce1ff3b7e7479cb2af10b209f07506
Autor:
John S. A. Mattick, Robin E. Bromley, Kaylee J. Watson, Ricky S. Adkins, Christopher I. Holt, Jarrett F. Lebov, Benjamin C. Sparklin, Tyonna S. Tyson, David A. Rasko, Julie C. Dunning Hotopp
Publikováno v:
mBio, Vol 15, Iss 10 (2024)
ABSTRACT RNA transcripts are potential therapeutic targets, yet bacterial transcripts have uncharacterized biodiversity. We developed an algorithm for transcript prediction called tp.py using it to predict transcripts (mRNA and other RNAs) in Escheri
Externí odkaz:
https://doaj.org/article/d0edc818c52849dfb46e0614f9812657
Autor:
Wang Liu-Wei, Wiep van der Toorn, Patrick Bohn, Martin Hölzer, Redmond P. Smyth, Max von Kleist
Publikováno v:
BMC Genomics, Vol 25, Iss 1, Pp 1-15 (2024)
Abstract Background Direct RNA sequencing (dRNA-seq) on the Oxford Nanopore Technologies (ONT) platforms can produce reads covering up to full-length gene transcripts, while containing decipherable information about RNA base modifications and poly-A
Externí odkaz:
https://doaj.org/article/cd3c7d0cec67474b96de101bc58c8145
Autor:
Jonathan S. Abebe, Yasmine Alwie, Erik Fuhrmann, Jonas Leins, Julia Mai, Ruth Verstraten, Sabrina Schreiner, Angus C. Wilson, Daniel P. Depledge
Publikováno v:
mSystems, Vol 9, Iss 7 (2024)
ABSTRACT Nanopore direct RNA sequencing (DRS) enables the capture and full-length sequencing of native RNAs, without recoding or amplification bias. Resulting data sets may be interrogated to define the identity and location of chemically modified ri
Externí odkaz:
https://doaj.org/article/6e0769599e5e491b9ab29a7c043b7473
Autor:
Feng Yu, Huanhuan Qi, Li Gao, Sen Luo, Rebecca Njeri Damaris, Yinggen Ke, Wenhua Wu, Pingfang Yang
Publikováno v:
Genomics, Proteomics & Bioinformatics, Vol 21, Iss 4, Pp 788-804 (2023)
Transcriptome analysis based on high-throughput sequencing of a cDNA library has been widely applied to functional genomic studies. However, the cDNA dependence of most RNA sequencing techniques constrains their ability to detect base modifications o
Externí odkaz:
https://doaj.org/article/37f958aa71b9467bbd1b147508f664f3
Autor:
Sean T. Bresnahan, Ellen Lee, Lindsay Clark, Rong Ma, Juliana Rangel, Christina M. Grozinger, Hongmei Li-Byarlay
Publikováno v:
BMC Genomics, Vol 24, Iss 1, Pp 1-13 (2023)
Abstract Conflict between genes inherited from the mother (matrigenes) and the father (patrigenes) is predicted to arise during social interactions among offspring if these genes are not evenly distributed among offspring genotypes. This intragenomic
Externí odkaz:
https://doaj.org/article/b25e1ff78936437bb08944d0f8fe2dde
Publikováno v:
Phytobiomes Journal, Vol 7, Iss 1, Pp 42-54 (2023)
Soil microbes play an undeniable role in sustainable agriculture, plant health, and soil management. A deeper understanding of soil microbial composition and function has been gained through next-generation sequencing. Although soil metagenomics has
Externí odkaz:
https://doaj.org/article/4288747fb5f744cc8910aefa94478465
Autor:
Longxian Chen, Liang Ou, Xinyun Jing, Yimeng Kong, Bingran Xie, Niubing Zhang, Han Shi, Hang Qin, Xuan Li, Pei Hao
Publikováno v:
Genome Biology, Vol 24, Iss 1, Pp 1-13 (2023)
Abstract Single-molecule detection and phasing of A-to-I RNA editing events remain an unresolved problem. Long-read and PCR-free nanopore native RNA sequencing offers a great opportunity for direct RNA editing detection. Here, we develop a neural net
Externí odkaz:
https://doaj.org/article/a401690c88e74036bc88f44bac80f1d9
Autor:
Lin Wang, Ling Shi, Yonghao Liang, Judy Kin-Wing Ng, Chan Hoi Yin, Lingyi Wang, Jinpao Hou, Yiwei Wang, Cathy Sin-Hang Fung, Peter Ka-Fung Chiu, Chi-Fai Ng, Stephen Kwok-Wing Tsui
Publikováno v:
Frontiers in Oncology, Vol 13 (2023)
Although the role of METTL3 has been extensively studied in many cancers, its role in isoform switching in prostate cancer (PCa) has been poorly explored. To investigate its role, we applied standard RNA-sequencing and long-read direct RNA-sequencing
Externí odkaz:
https://doaj.org/article/1c47217957d2461dbf110a800193e01d