Zobrazeno 1 - 10
of 51
pro vyhledávání: '"Dipali G. Sashital"'
Autor:
Dipali G. Sashital
Publikováno v:
Genome Medicine, Vol 10, Iss 1, Pp 1-4 (2018)
Editorial summary CRISPR–Cas systems have provided revolutionary tools for genome editing. The discovery of Cas proteins with alternative activities has now enabled sensitive and robust tools for detecting nucleic acids. Recent reports harnessing t
Externí odkaz:
https://doaj.org/article/fadbe29496b2484baf1fd7a4b3384c1b
Publikováno v:
Cell Reports, Vol 21, Iss 13, Pp 3717-3727 (2017)
CRISPR-Cas systems defend bacteria and archaea against infection by bacteriophage and other threats. The central component of these systems are surveillance complexes that use guide RNAs to bind specific regions of foreign nucleic acids, marking them
Externí odkaz:
https://doaj.org/article/4e7608405aa1403f89895335b391f4b2
Publikováno v:
PLoS Biology, Vol 21, Iss 4, p e3002065 (2023)
CRISPR-mediated interference relies on complementarity between a guiding CRISPR RNA (crRNA) and target nucleic acids to provide defense against bacteriophage. Phages escape CRISPR-based immunity mainly through mutations in the protospacer adjacent mo
Externí odkaz:
https://doaj.org/article/7bb3d5b18739470cbab4690808c2ebcd
Autor:
Michael A Schelling, Dipali G Sashital
Publikováno v:
eLife, Vol 9 (2020)
The response of bacteria to the threat posed by phages depends on their local environment.
Externí odkaz:
https://doaj.org/article/61942af203f0487f91c16183cf4f48a7
Autor:
Hayun, Lee, Dipali G, Sashital
Publikováno v:
Trends Biochem Sci
Prokaryotes use clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated (Cas) proteins as an adaptive immune system. CRISPR-Cas systems preserve molecular memories of infections by integrating short fragments of foreig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb087a1cc801ef3188e320b23ddb50f0
https://doi.org/10.1016/j.tibs.2022.02.004
https://doi.org/10.1016/j.tibs.2022.02.004
Publikováno v:
eLife, Vol 8 (2019)
CRISPR adaptation immunizes bacteria and archaea against viruses. During adaptation, the Cas1-Cas2 complex integrates fragments of invader DNA as spacers in the CRISPR array. Recently, an additional protein Cas4 has been implicated in selection and p
Externí odkaz:
https://doaj.org/article/94c1e7a435ac44a4b050f886f894d16d
Autor:
Andrew J. Severin, Shravanti K Suresh, Karthik Murugan, Arun S. Seetharam, Dipali G. Sashital
Publikováno v:
Nucleic Acids Research
Cas9 is an RNA-guided endonuclease in the bacterial CRISPR–Cas immune system and a popular tool for genome editing. The commonly used Streptococcus pyogenes Cas9 (SpCas9) is relatively non-specific and prone to off-target genome editing. Other Cas9
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13b9f284e368c60e7727ca291c1a25af
https://doi.org/10.1093/nar/gkab163
https://doi.org/10.1093/nar/gkab163
CRISPR-mediated interference relies on complementarity between a guiding CRISPR RNA (crRNA) and target nucleic acids to provide defense against bacteriophage. Phages escape CRISPR-based immunity mainly through mutations in the PAM and seed regions. H
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::288c42058dd8977deab7226af6f0926c
https://doi.org/10.1101/2022.05.30.494023
https://doi.org/10.1101/2022.05.30.494023
Publikováno v:
Current opinion in structural biology. 77
CRISPR-associated (Cas) endonucleases specifically target and cleave RNA or DNA based on complementarity to a guide RNA. Cas endonucleases - including Cas9, Cas12a, and Cas13 - have been adopted for a wide array of biotechnological tools, including g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dcfee00e796fcaa9f8ecea0a3c714647
https://pubmed.ncbi.nlm.nih.gov/36170778
https://pubmed.ncbi.nlm.nih.gov/36170778
Autor:
Dipali G Sashital, Candacia A Greeman, Dmitry Lyumkis, Clinton S Potter, Bridget Carragher, James R Williamson
Publikováno v:
eLife, Vol 3 (2014)
Ribosome assembly is a complex process involving the folding and processing of ribosomal RNAs (rRNAs), concomitant binding of ribosomal proteins (r-proteins), and participation of numerous accessory cofactors. Here, we use a quantitative mass spectro
Externí odkaz:
https://doaj.org/article/6122f046f4ff4c399fbb006b5193cb9b