Zobrazeno 1 - 10
of 51
pro vyhledávání: '"Dipali G. Sashital"'
Autor:
Dipali G. Sashital
Publikováno v:
Genome Medicine, Vol 10, Iss 1, Pp 1-4 (2018)
Editorial summary CRISPR–Cas systems have provided revolutionary tools for genome editing. The discovery of Cas proteins with alternative activities has now enabled sensitive and robust tools for detecting nucleic acids. Recent reports harnessing t
Externí odkaz:
https://doaj.org/article/fadbe29496b2484baf1fd7a4b3384c1b
Publikováno v:
Cell Reports, Vol 21, Iss 13, Pp 3717-3727 (2017)
CRISPR-Cas systems defend bacteria and archaea against infection by bacteriophage and other threats. The central component of these systems are surveillance complexes that use guide RNAs to bind specific regions of foreign nucleic acids, marking them
Externí odkaz:
https://doaj.org/article/4e7608405aa1403f89895335b391f4b2
Publikováno v:
PLoS Biology, Vol 21, Iss 4, p e3002065 (2023)
CRISPR-mediated interference relies on complementarity between a guiding CRISPR RNA (crRNA) and target nucleic acids to provide defense against bacteriophage. Phages escape CRISPR-based immunity mainly through mutations in the protospacer adjacent mo
Externí odkaz:
https://doaj.org/article/7bb3d5b18739470cbab4690808c2ebcd
Autor:
Hayun, Lee, Dipali G, Sashital
Publikováno v:
Trends Biochem Sci
Prokaryotes use clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated (Cas) proteins as an adaptive immune system. CRISPR-Cas systems preserve molecular memories of infections by integrating short fragments of foreig
Autor:
Courtney Davis-Vogel, Brandon Van Allen, John L Van Hemert, Amit Sethi, Mark E Nelson, Dipali G Sashital
Publikováno v:
PLoS ONE, Vol 13, Iss 9, p e0203160 (2018)
RNA interference (RNAi)-based technology shows great potential for use in agriculture, particularly for management of costly insect pests. In the decade since the insecticidal effects of environmentally-introduced RNA were first reported, this treatm
Externí odkaz:
https://doaj.org/article/82aaa8c273f04014a9c1798ad00d6a55
CRISPR-mediated interference relies on complementarity between a guiding CRISPR RNA (crRNA) and target nucleic acids to provide defense against bacteriophage. Phages escape CRISPR-based immunity mainly through mutations in the PAM and seed regions. H
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::288c42058dd8977deab7226af6f0926c
https://doi.org/10.1101/2022.05.30.494023
https://doi.org/10.1101/2022.05.30.494023
Publikováno v:
Current opinion in structural biology. 77
CRISPR-associated (Cas) endonucleases specifically target and cleave RNA or DNA based on complementarity to a guide RNA. Cas endonucleases - including Cas9, Cas12a, and Cas13 - have been adopted for a wide array of biotechnological tools, including g
Autor:
Andrew J. Severin, Shravanti K Suresh, Karthik Murugan, Arun S. Seetharam, Dipali G. Sashital
Publikováno v:
Nucleic Acids Research
Cas9 is an RNA-guided endonuclease in the bacterial CRISPR–Cas immune system and a popular tool for genome editing. The commonly used Streptococcus pyogenes Cas9 (SpCas9) is relatively non-specific and prone to off-target genome editing. Other Cas9
Publikováno v:
The FASEB Journal. 35