High-Power Near-Concentric Fabry-Perot Cavity for Phase Contrast Electron Microscopy

Autor: Turnbaugh, Carter, Axelrod, Jeremy J., Campbell, Sara L., Dioquino, Jeske Y., Petrov, Petar N., Remis, Jonathan, Schwartz, Osip, Yu, Zanlin, Cheng, Yifan, Glaeser, Robert M., Mueller, Holger

Transmission electron microscopy (TEM) of vitrified biological macromolecules (cryo-EM) is limited by the weak phase contrast signal that is available from such samples. Using a phase plate would thus substantially improve the signal-to-noise ratio.

Externí odkaz: http://arxiv.org/abs/2012.08638

High-power near-concentric Fabry-Perot cavity for phase contrast electron microscopy.

Autor: Turnbaugh C; Department of Physics, 366 Physics MS 7300, University of California-Berkeley, Berkeley, California 94720, USA., Axelrod JJ; Department of Physics, 366 Physics MS 7300, University of California-Berkeley, Berkeley, California 94720, USA., Campbell SL; Department of Physics, 366 Physics MS 7300, University of California-Berkeley, Berkeley, California 94720, USA., Dioquino JY; Department of Physics, 366 Physics MS 7300, University of California-Berkeley, Berkeley, California 94720, USA., Petrov PN; Department of Physics, 366 Physics MS 7300, University of California-Berkeley, Berkeley, California 94720, USA., Remis J; California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, California 94720, USA., Schwartz O; Department of Physics of Complex Systems, Weizmann Institute of Science, Rehovot 7610001, Israel., Yu Z; Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California 94158, USA., Cheng Y; Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California 94158, USA., Glaeser RM; Lawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley, California 94720, USA., Mueller H; Department of Physics, 366 Physics MS 7300, University of California-Berkeley, Berkeley, California 94720, USA.

Publikováno v: The Review of scientific instruments [Rev Sci Instrum] 2021 May 01; Vol. 92 (5), pp. 053005.