Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Dinh H Diep"'
This protocol was adapted for the isolation of single nuclei from frozen skeletal and cardiac muscle tissues for molecular characterization with the SNARE-seq2, sci-ATAC-seq, and snRNA-seq assays. References: (1) Preissl et al (2015). Circulation Res
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bde2d2fec4e8594ec0920c0f78dbf514
https://doi.org/10.17504/protocols.io.b2z4qf8w
https://doi.org/10.17504/protocols.io.b2z4qf8w
To study the heterogeneity of complex tissues by joint profiling of gene expression and its regulation, we require an accurate and high-throughput method. Here we described improved high-throughput combinatorial indexing-based single-nucleus chromati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fdb53b916d79b6eb74b68f10898c9e56
https://doi.org/10.17504/protocols.io.bzdsp26e
https://doi.org/10.17504/protocols.io.bzdsp26e
To study the heterogeneity of complex tissues by joint profiling of gene expression and its regulation, we require an accurate and high-throughput method. Here we described improved high-throughput combinatorial indexing-based single-nucleus chromati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4f444082504614214d616d30b4326b56
https://doi.org/10.17504/protocols.io.bzdrp256
https://doi.org/10.17504/protocols.io.bzdrp256
Isolation of nuclei from frozen human skeletal muscle for single nucleus RNA and chromatin assays v1
This protocol was adapted for the isolation of single nuclei from frozen skeletal muscle tissues for molecular characterization with the SNARE-seq2 assay. References: (1) Preissl et al (2015). Circulation Research. Doi: 10.1161/CIRCRESAHA.115.306337
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5dea76e34133d5cb7f8aeebfd9ff1e40
https://doi.org/10.17504/protocols.io.bv9dn926
https://doi.org/10.17504/protocols.io.bv9dn926
Autor:
Blue Lake, Elizabeth Duong, Dinh H Diep, Xin Sun, Peter K, Jim Hagood, Gloria S Pryhuber, Sanjay Jain, Kun Zhang [University Of California
This document provides an overview of the protocols used by the Human Kidney, Urinary Tract, and Lung Mapping Center (KULMAP) for generation of spatially resolved mulitomic cell type organ maps within HuBMAP. This involves sequencing of the transcrip
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f2025dc0aace7c0415715b6376887317
https://doi.org/10.17504/protocols.io.bvvin64e
https://doi.org/10.17504/protocols.io.bvvin64e
Nuclei can be readily isolated from frozen tissues with a combination of chemical and physical treatments that can circumvent the non-uniform or incomplete dissociation of solid tissues into single cells. The isolation of nuclei can also circumvent R
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c442d44db73195344f01ca454974b8d9
https://doi.org/10.17504/protocols.io.bu7unznw
https://doi.org/10.17504/protocols.io.bu7unznw
Autor:
Blue Lake, Elizabeth Duong, Dinh H Diep, Xin Sun, Peter K, Jim Hagood, Gloria S Pryhuber, Sanjay Jain, Kun Zhang [University Of California
This document provides an overview of the protocols used by the Human Kidney, Urinary Tract, and Lung Mapping Center (KULMAP) for generation of spatially resolved mulitomic cell type organ maps within HuBMAP. This involves sequencing of the transcrip
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::00ba6deb34c297dc39b5830e800fe98b
https://doi.org/10.17504/protocols.io.bj9wkr7e
https://doi.org/10.17504/protocols.io.bj9wkr7e
To study the heterogeneity of complex tissues by joint profiling of gene expression and its regulation, we require an accurate and high-throughput method. Here we described improved high-throughput combinatorial indexing-based single-nucleus chromati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0a1e42c93f41c9fc16e2c5326f3accad
https://doi.org/10.17504/protocols.io.be5gjg3w
https://doi.org/10.17504/protocols.io.be5gjg3w