Zobrazeno 1 - 10
of 84
pro vyhledávání: '"Dimitre G Ouzounov"'
Autor:
Tianyu Wang, Chunyan Wu, Dimitre G Ouzounov, Wenchao Gu, Fei Xia, Minsu Kim, Xusan Yang, Melissa R Warden, Chris Xu
Publikováno v:
eLife, Vol 9 (2020)
1300 nm three-photon calcium imaging has emerged as a useful technique to allow calcium imaging in deep brain regions. Application to large-scale neural activity imaging entails a careful balance between recording fidelity and perturbation to the sam
Externí odkaz:
https://doaj.org/article/bb1f5a298fca4712ae992c821a4f0f79
Autor:
Aaron T. Mok, Tianyu Wang, Shitong Zhao, Kristine E. Kolkman, Danni Wu, Dimitre G. Ouzounov, Changwoo Seo, Chunyan Wu, Joseph R. Fetcho, Chris Xu
Publikováno v:
eLight, Vol 4, Iss 1, Pp 1-14 (2024)
Abstract In vivo imaging of large-scale neuronal activity plays a pivotal role in unraveling the function of the brain's circuitry. Multiphoton microscopy, a powerful tool for deep-tissue imaging, has received sustained interest in advancing its spee
Externí odkaz:
https://doaj.org/article/d13c4a3b9eea4ebd9a9100ad8a630afd
Autor:
Kibaek Choe, Yusaku Hontani, Tianyu Wang, Eric Hebert, Dimitre G. Ouzounov, Kristine Lai, Ankur Singh, Wendy Béguelin, Ari M. Melnick, Chris Xu
Publikováno v:
Nat Immunol
Intravital confocal microscopy and two-photon microscopy are powerful tools to explore the dynamic behavior of immune cells in mouse lymph nodes (LNs), with penetration depth of ~100 and ~300 μm, respectively. Here, we used intravital three-photon m
Autor:
David Sinefeld, Fei Xia, Mengran Wang, Tianyu Wang, Chunyan Wu, Hari P. Paudel, Dimitre G. Ouzounov, Thomas G. Bifano, Chris Xu
Publikováno v:
2022 IEEE Photonics Conference (IPC).
Autor:
Andrew H. Bass, Kristine E. Kolkman, Rose L. Tatarsky, David Sinefeld, Dimitre G. Ouzounov, Najva Akbari, Dawnis M. Chow, Chris Xu, Joseph R. Fetcho
Publikováno v:
Nature methods
Behaviors emerge from activity throughout the brain, but non-invasive optical access in adult vertebrate brains is limited. We show that three-photon (3P) imaging through the head of intact adult zebrafish allows structural and functional imaging at
Autor:
David Sinefeld, Fei Xia, Mengran Wang, Tianyu Wang, Chunyan Wu, Xusan Yang, Hari P. Paudel, Dimitre G. Ouzounov, Thomas G. Bifano, Chris Xu
Publikováno v:
Frontiers in neuroscience. 16
Three-photon microscopy (3PM) was shown to allow deeper imaging than two-photon microscopy (2PM) in scattering biological tissues, such as the mouse brain, since the longer excitation wavelength reduces tissue scattering and the higher-order non-line
Autor:
Melissa R. Warden, Fei Xia, Chris Xu, Minsu Kim, Dimitre G. Ouzounov, Xusan Yang, Chunyan Wu, Tianyu Wang, Wenchao Gu
Publikováno v:
eLife
eLife, Vol 9 (2020)
eLife, Vol 9 (2020)
1300-nm three-photon calcium imaging has emerged as a useful technique to allow calcium imaging in deep brain regions. Application to large-scale neural activity imaging entails a careful balance between recording fidelity and tissue heating. We calc
Autor:
Hyung Song Nam, Chunyan Wu, Melissa R. Warden, Bo Li, Chris Xu, Mengran Wang, Mario R. Capecchi, Akash Guru, Tianyu Wang, David Sinefeld, Dimitre G. Ouzounov
Publikováno v:
Multiphoton Microscopy in the Biomedical Sciences XVIII.
We demonstrate three-photon microscopy (3PM) of mouse cerebellum at 1 mm depth by imaging both blood vessels and neurons. We compared 3PM and 2PM in the mouse cerebellum for imaging green (using excitation sources at 1300 nm and 920 nm, respectively)
Autor:
Dimitre G. Ouzounov, Mark J. Schnitzer, Yanping Zhang, Nicholas G. Horton, Chris Xu, Tianyu Wang, Chunyan Wu, Bin Zhang, Cheng-Hsun Wu
Publikováno v:
Nature methods
Optical imaging through the intact mouse skull is challenging because of skull-induced aberrations and scattering. We found that three-photon excitation provided improved optical sectioning compared with that obtained with two-photon excitation, even