Kinetic modeling as a tool to understand the influence of cell culture process parameters on the glycation of monoclonal antibody biotherapeutics

Autor: Rahul Pradhan, Diane Hatton, Nitin Agarwal, Viv Lindo, Guillermo Miro-Quesada, James Savery, Jan Kemper, Jihong Wang, Dimitra Serfiotis-Mitsa, Alison Mason, Sanjeev Ahuja, Allen D. Bosley

Publikováno v: Biotechnology progressREFERENCES. 35(5)

Glycation, the nonenzymatic reaction between the reducing sugar glucose and the primary amine residues on amino acid side chains, commonly occurs in the cell culture supernatant during production of therapeutic monoclonal antibodies (mAbs). While gly

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e78576925f4515148fc53701127bf048
https://pubmed.ncbi.nlm.nih.gov/31180183

The structure of the KlcA and ArdB proteins reveals a novel fold and antirestriction activity against Type I DNA restriction systems in vivo but not in vitro

Autor: Gareth A. Roberts, John H. White, Garry W. Blakely, David T. F. Dryden, Dušan Uhrín, Andrew P. Herbert, Dinesh C. Soares, Dimitra Serfiotis-Mitsa

Publikováno v: Serfiotis-Mitsa, D, Herbert, A P, Roberts, G A, Soares, D C, White, J H, Blakely, G W, Uhrin, D & Dryden, D T F 2010, ' The structure of the KlcA and ArdB proteins reveals a novel fold and antirestriction activity against Type I DNA restriction systems in vivo but not in vitro ', Nucleic Acids Research, vol. 38, no. 5, pp. 1723-1737 . https://doi.org/10.1093/nar/gkp1144
Nucleic Acids Research

Plasmids, conjugative transposons and phage frequently encode anti-restriction proteins to enhance their chances of entering a new bacterial host that is highly likely to contain a Type I DNA restriction and modification (RM) system. The RM system us

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ad4663a74f9ba021e7812fef0a464c0
https://hdl.handle.net/20.500.11820/144c51da-566e-4200-aba5-fa91040ddc57