Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Dileep K. Pulukkunat"'
Publikováno v:
Journal of Molecular Biology. 393:1043-1055
Ribonuclease P (RNase P) is a ribonucleoprotein (RNP) enzyme that catalyzes the Mg(2+)-dependent 5' maturation of precursor tRNAs. In all domains of life, it is a ribozyme: the RNase P RNA (RPR) component has been demonstrated to be responsible for c
Publikováno v:
Nucleic Acids Research
Metagenomics has been employed to systematically sequence, classify, analyze and manipulate the entire genetic material isolated from environmental samples. Finding genes within metagenomic sequences remains a formidable challenge, and noncoding RNA
Publikováno v:
Proceedings of the National Academy of Sciences. 103:16147-16152
RNase P, which catalyzes the magnesium-dependent 5′-end maturation of tRNAs in all three domains of life, is composed of one essential RNA and a varying number of protein subunits depending on the source: at least one in bacteria, four in archaea,
Publikováno v:
Plant Physiology. 125:1187-1190
The objective of this investigation is to examine the potential for using plant ribonuclease P (RNase P) as a tool for cleaving target mRNAs and thereby disrupting gene expression in plants. RNase P is a ubiquitous ribonucleoprotein that is uniquely
Autor:
Shalom J. Wind, Ruben L. Gonzalez, Dileep K. Pulukkunat, Matteo Palma, Colin D. Kinz-Thompson, James Hone, Daniel Chenet
Publikováno v:
ACS nano. 7(9)
The optical confinement generated by metal-based nanoapertures fabricated on a silica substrate has recently enabled single-molecule fluorescence measurements to be performed at physiologically relevant background concentrations of fluorophore-labele
Publikováno v:
Nucleic Acids Research
RNase P catalyzes the Mg(2)(+)-dependent 5'-maturation of precursor tRNAs. Biochemical studies on the bacterial holoenzyme, composed of one catalytic RNase P RNA (RPR) and one RNase P protein (RPP), have helped understand the pleiotropic roles (inclu
Autor:
Samuel H. Sternberg, Michael T. Englander, Dileep K. Pulukkunat, Jiangning Wang, Margaret M. Elvekrog, Jingyi Fei, Daniel D. MacDougall, Ruben L. Gonzalez
Single-molecule fluorescence resonance energy transfer (smFRET) has emerged as a powerful tool for mechanistic investigations of increasingly complex biochemical systems. Recently, we and others have successfully used smFRET to directly investigate t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::309f5e233632fc17a5a389c5bad4c8ad
https://doi.org/10.1016/s0076-6879(10)72008-5
https://doi.org/10.1016/s0076-6879(10)72008-5
Autor:
Dileep K. Pulukkunat, Venkat Gopalan
Publikováno v:
Nucleic Acids Research
Ribonuclease P (RNase P), a ribonucleoprotein (RNP) complex required for tRNA maturation, comprises one essential RNA (RPR) and protein subunits (RPPs) numbering one in bacteria, and at least four in archaea and nine in eukarya. While the bacterial R
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 236
As we trek into the uncharted territories of the genomic era, there is an urgency for the development of approaches for assigning functions to the multitude of uncharacterized genes. Although currently available knock-out methodologies could be used