The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase

Autor: Alkema, WBL, Dijkhuis, AJ, de Vries, E, Janssen, DB

Publikováno v: European Journal of Biochemistry, 269(8), 2093-2100. Blackwell Publishing Ltd

Penicillin acylase of Escherichia colt catalyses the hydrolysis and synthesis of beta-lactam antibiotics. To study the role of hydrophobic residues in these reactions, we have mutated three active-site phenylalanines. Mutation of alphaF146, betaF24 a

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https://research.rug.nl/en/publications/f9e5c976-dacd-424b-8f21-697fef95e41a

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RhoA Activation Sensitizes Cells to Proteotoxic Stimuli by Abrogating the HSF1-Dependent Heat Shock Response.

Autor: Meijering RA; Department of Clinical Pharmacy and Pharmacology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., Wiersma M; Department of Clinical Pharmacy and Pharmacology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., van Marion DM; Department of Clinical Pharmacy and Pharmacology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., Zhang D; Department of Clinical Pharmacy and Pharmacology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., Hoogstra-Berends F; Department of Clinical Pharmacy and Pharmacology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., Dijkhuis AJ; Department of Cell Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., Schmidt M; Department of Molecular Pharmacology, University of Groningen, Groningen, The Netherlands., Wieland T; Institute of Experimental and Clinical Pharmacology and Toxicology, Mannheim Medical Faculty, University of Heidelberg, Mannheim, Germany., Kampinga HH; Department of Cell Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., Henning RH; Department of Clinical Pharmacy and Pharmacology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands., Brundel BJ; Department of Clinical Pharmacy and Pharmacology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands; Department of Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands.

Publikováno v: PloS one [PLoS One] 2015 Jul 20; Vol. 10 (7), pp. e0133553. Date of Electronic Publication: 2015 Jul 20 (Print Publication: 2015).

Calpain mediates cardiac troponin degradation and contractile dysfunction in atrial fibrillation.

Autor: Ke L; Department of Radiation and Stress Cell Biology, University Institute for Drug Exploration (GUIDE), University of Groningen, University Medical Center Groningen, The Netherlands., Qi XY, Dijkhuis AJ, Chartier D, Nattel S, Henning RH, Kampinga HH, Brundel BJ

Publikováno v: Journal of molecular and cellular cardiology [J Mol Cell Cardiol] 2008 Nov; Vol. 45 (5), pp. 685-93. Date of Electronic Publication: 2008 Sep 11.

Gangliosides do not affect ABC transporter function in human neuroblastoma cells.

Autor: Dijkhuis AJ; Department of Cell Biology, Section of Membrane Cell Biology, University Medical Center Groningen, 9713 AV Groningen, The Netherlands., Klappe K, Kamps W, Sietsma H, Kok JW

Publikováno v: Journal of lipid research [J Lipid Res] 2006 Jun; Vol. 47 (6), pp. 1187-95. Date of Electronic Publication: 2006 Mar 18.