Zobrazeno 1 - 10
of 125
pro vyhledávání: '"Dietmar Porschke"'
Autor:
Dietmar Porschke
Publikováno v:
European Biophysics Journal
Because of open problems in the relation between results obtained by relaxation experiments and molecular dynamics simulations on the B-A transition of DNA, relaxation measurements of the B-A dynamics have been extended to a wider range of conditions
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::058b951d7956ab42a9cca3f5e5eef9ae
https://hdl.handle.net/21.11116/0000-0001-9388-F21.11116/0000-0001-9386-1
https://hdl.handle.net/21.11116/0000-0001-9388-F21.11116/0000-0001-9386-1
Autor:
Dietmar Porschke
Publikováno v:
European Biophysics Journal
Because of repeated claims that A-DNA cannot exist without aggregation or condensation, the state of DNA restriction fragments with 84–859 bp has been analyzed in aqueous solutions upon reduction of the water activity. Rotational diffusion times τ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dde91496002297bdefc4b756febcff06
https://hdl.handle.net/11858/00-001M-0000-002A-E766-311858/00-001M-0000-002A-E764-7
https://hdl.handle.net/11858/00-001M-0000-002A-E766-311858/00-001M-0000-002A-E764-7
Autor:
Dietmar Porschke
Publikováno v:
European Biophysics Journal
The kinetics of cAMP receptor (CAP) binding to promoter DNA has been studied by stopped-flow electric-dichroism at a reduced salt concentration, where the coupling of non-specific and specific binding can be observed directly. Amplitudes, rise and de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e9eca5a10b1713b2a5698018518e523
https://doi.org/10.1007/s00249-012-0791-y
https://doi.org/10.1007/s00249-012-0791-y
Autor:
Dietmar Porschke
Publikováno v:
Biochemistry. 49:5553-5559
The structure of the cyclic AMP receptor-promoter complex in solution was studied in the range of 0.2-50 microM cAMP by measurements of the electric birefringence at 0.1 M salt using a lac promoter DNA with 121 bp and with the CAP binding site at its
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4aa98656462da7508d0b40bb696982f3
https://doi.org/10.1021/bi100542f
https://doi.org/10.1021/bi100542f
Autor:
Dietmar Porschke, Davis Jose
Publikováno v:
Journal of the American Chemical Society. 127:16120-16128
The dynamics of the B-A transition of DNA double helices with different GC contents and various chain lengths has been characterized by an electric field pulse technique. The field-induced B-A reaction is separated from orientation effects using the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a07d1955f245c9e6f926d721444ce076
https://doi.org/10.1021/ja053691d
https://doi.org/10.1021/ja053691d
Publikováno v:
Nucleic Acids Research. 28:4428-4434
The dynamics of a hammerhead ribozyme was analyzed by measurements of fluorescence-detected temperature jump relaxation. The ribozyme was substituted at different positions by 2-aminopurine (2-AP) as fluorescence indicator; these substitutions do not
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd019eb49c5a408d240a8555a526d178
https://doi.org/10.1093/nar/28.22.4428
https://doi.org/10.1093/nar/28.22.4428
Publikováno v:
Biochemistry. 39:4500-4507
The dynamics of RNA hairpin tetraloops of the GNRA type [sequence G- any ribonucleotide (N)-purine (R)-A] was analyzed by fluorescence spectroscopy and by fluorescence-detected temperature-jump relaxation, using RNA oligomers with 2-aminopurine (2AP)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ac8aec674f3c4c76018ebe1bc51ae0e
https://doi.org/10.1021/bi992297n
https://doi.org/10.1021/bi992297n
Publikováno v:
Journal of Molecular Biology. 289:799-813
Global structure and flexibility of three different hairpin ribozyme constructs have been analyzed by measuring their electric dichroism decay in various buffers at temperatures between 2 and 30 degrees C. The hairpin ribozyme is characterized by two
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::afc3cae4a4b9625f3bcd35cbb30deb89
https://doi.org/10.1006/jmbi.1999.2777
https://doi.org/10.1006/jmbi.1999.2777
Autor:
Dietmar Porschke
Publikováno v:
Biophysical Journal. 75:528-537
A stopped-flow field-jump instrument and its use for the analysis of macromolecular structure changes during reactions is described. The operation of the new instrument is simple and reliable, owing to a new type of cell construction with electrodes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b31e299bd06982a888c2681cc4fc2ba1
https://doi.org/10.1016/s0006-3495(98)77542-3
https://doi.org/10.1016/s0006-3495(98)77542-3