Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Dierk Roessner"'
Autor:
Alina Kulakova, Sowmya Indrakumar, Pernille Sønderby, Lorenzo Gentiluomo, Werner Streicher, Dierk Roessner, Wolfgang Frieß, Günther H.J. Peters, Pernille Harris
Publikováno v:
Journal of Structural Biology: X, Vol 4, Iss , Pp 100017- (2020)
Transferrin is an attractive candidate for drug delivery due to its ability to cross the blood brain barrier. However, in order to be able to use it for therapeutic purposes, it is important to investigate how its stability depends on different formu
Externí odkaz:
https://doaj.org/article/a92acadb0a5249629cba80c8d938c834
Autor:
Alina Kulakova, Dillen Augustijn, Inas El Bialy, Lorenzo Gentiluomo, Maria Laura Greco, Stefan Hervø-Hansen, Sowmya Indrakumar, Sujata Mahapatra, Marcello Martinez Morales, Christin Pohl, Marco Polimeni, Aisling Roche, Hristo L. Svilenov, Andreas Tosstorff, Matja Zalar, Robin Curtis, Jeremy P. Derrick, Wolfgang Frieß, Alexander P. Golovanov, Mikael Lund, Allan Nørgaard, Tarik A. Khan, Günther H. J. Peters, Alain Pluen, Dierk Roessner, Werner W. Streicher, Christopher F. van der Walle, Jim Warwicker, Shahid Uddin, Gerhard Winter, Jens Thostrup Bukrinski, Åsmund Rinnan, Pernille Harris
Publikováno v:
Molecular Pharmaceutics.
Autor:
Sujata Mahapatra, Marco Polimeni, Lorenzo Gentiluomo, Dierk Roessner, Wolfgang Frieß, Günther H. J. Peters, Werner W. Streicher, Mikael Lund, Pernille Harris
Publikováno v:
Molecular Pharmaceutics
Using light scattering (LS), small-angle X-ray scattering (SAXS), and coarse-grained Monte Carlo (MC) simulations, we studied the self-interactions of two monoclonal antibodies (mAbs), PPI03 and PPI13. With LS measurements, we obtained the osmotic se
Autor:
Stuart Macaulay, Pavel Kulich, Alena Vrablikova, Dierk Roessner, Renata Hezova, František Hubatka, Jaroslav Turánek, Milan Raska, Jan Kotouček, Ivan Psikal
Publikováno v:
Analytical and Bioanalytical Chemistry
Porcine circovirus causes the post-weaning multi-systemic wasting syndrome. Despite the existence of commercial vaccines, the development of more effective and cheaper vaccines is expected. The usage of chimeric antigens allows serological differenti
Autor:
Wolfgang Frieß, Dierk Roessner, Lorenzo Gentiluomo, Werner Streicher, Sujata Mahapatra, Pernille Harris
Publikováno v:
Journal of Pharmaceutical Sciences
Gentiluomo, L, Roessner, D, Streicher, W, Mahapatra, S, Harris, P & Frieß, W 2020, ' Characterization of native reversible self-association of a monoclonal antibody mediated by Fab-Fab interaction ', Journal of Pharmaceutical Sciences, vol. 109, no. 1, pp. 443-451 . https://doi.org/10.1016/j.xphs.2019.09.021
Gentiluomo, L, Roessner, D, Streicher, W, Mahapatra, S, Harris, P & Frieß, W 2020, ' Characterization of native reversible self-association of a monoclonal antibody mediated by Fab-Fab interaction ', Journal of Pharmaceutical Sciences, vol. 109, no. 1, pp. 443-451 . https://doi.org/10.1016/j.xphs.2019.09.021
The native reversible self-association of monoclonal antibodies has been associated with high viscosity, liquid-liquid, and liquid-solid phase separation. We investigated the native reversible self-association of an IgG1, which exerts this associatio
Autor:
Robin Curtis, Nicole Sibanda, Dierk Roessner, Steven P. Trainoff, Wolfgang Friess, Aisling Roche, Lorenzo Gentiluomo
Publikováno v:
Journal of Colloid and Interface Science
Roche, A, Gentiluomo, L, Sibanda, N, Roessner, D, Friess, W, Trainoff, S P & Curtis, R 2022, ' Towards an improved prediction of concentrated antibody solution viscosity using the Huggins coefficient ', Journal of Colloid and Interface Science, vol. 607, no. Part 2, pp. 1813-1824 . https://doi.org/10.1016/j.jcis.2021.08.191
Roche, A, Gentiluomo, L, Sibanda, N, Roessner, D, Friess, W, Trainoff, S P & Curtis, R 2022, ' Towards an improved prediction of concentrated antibody solution viscosity using the Huggins coefficient ', Journal of Colloid and Interface Science, vol. 607, no. Part 2, pp. 1813-1824 . https://doi.org/10.1016/j.jcis.2021.08.191
The viscosity of a monoclonal antibody solution must be monitored and controlled as it can adversely affect product processing, packaging and administration. Engineering low viscosity mAb formulations is challenging as prohibitive amounts of material
Autor:
Shahid Uddin, Christin Pohl, Christopher F. van der Walle, Inas El Bialy, Jeremy P. Derrick, Andreas Tosstorff, Robin Curtis, Sujata Mahapatra, Werner Streicher, Dillen Augustijn, Aisling Roche, Alina Kulakova, Wolfgang Frieß, Åsmund Rinnan, Hristo L. Svilenov, Dierk Roessner, Lorenzo Gentiluomo, Sowmya Indrakumar, Alain Pluen, Gerhard Winter, Pernille Harris, Allan Nørgaard, Günther H.J. Peters, Maria Laura Greco, Marcello Martinez Morales, Tarik A. Khan
Publikováno v:
Molecular Pharmaceutics
Gentiluomo, L, Svilenov, H L, Augustijn, D, El Bialy, I, Greco, M L, Vitaliyivna Kulakova, A, Indrakumar, S, Mahapatra, S, Morales, M M, Pohl, C, Roche, A, Tosstorff, A, Curtis, R, Derrick, J P, Nørgaard, A, Khan, T A, Peters, G H J, Pluen, A, Rinnan, Å, Streicher, W W, van der Walle, C F, Uddin, S, Winter, G, Roessner, D, Harris, P & Frieß, W 2020, ' Advancing Therapeutic Protein Discovery and Development through Comprehensive Computational and Biophysical Characterization ', Molecular Pharmaceutics, vol. 17, no. 2, pp. 426-440 . https://doi.org/10.1021/acs.molpharmaceut.9b00852
Gentiluomo, L, Svilenov, H L, Augustijn, D, El Bialy, I, Greco, M L, Kulakova, A, Indrakumar, S, Mahapatra, S, Morales, M M, Pohl, C, Roche, A, Tosstorff, A, Curtis, R, Derrick, J P, Nørgaard, A, Khan, T A, Peters, G H J, Pluen, A, Rinnan, Å, Streicher, W W, van der Walle, C F, Uddin, S, Winter, G, Roessner, D, Harris, P & Friess, W 2020, ' Advancing Therapeutic Protein Discovery and Development through Comprehensive Computational and Biophysical Characterization ', Molecular Pharmaceutics, vol. 17, no. 2, pp. 426-440 . https://doi.org/10.1021/acs.molpharmaceut.9b00852
Gentiluomo, L, Svilenov, H L, Augustijn, D, El Bialy, I, Greco, M L, Vitaliyivna Kulakova, A, Indrakumar, S, Mahapatra, S, Morales, M M, Pohl, C, Roche, A, Tosstorff, A, Curtis, R, Derrick, J P, Nørgaard, A, Khan, T A, Peters, G H J, Pluen, A, Rinnan, Å, Streicher, W W, van der Walle, C F, Uddin, S, Winter, G, Roessner, D, Harris, P & Frieß, W 2020, ' Advancing Therapeutic Protein Discovery and Development through Comprehensive Computational and Biophysical Characterization ', Molecular Pharmaceutics, vol. 17, no. 2, pp. 426-440 . https://doi.org/10.1021/acs.molpharmaceut.9b00852
Gentiluomo, L, Svilenov, H L, Augustijn, D, El Bialy, I, Greco, M L, Kulakova, A, Indrakumar, S, Mahapatra, S, Morales, M M, Pohl, C, Roche, A, Tosstorff, A, Curtis, R, Derrick, J P, Nørgaard, A, Khan, T A, Peters, G H J, Pluen, A, Rinnan, Å, Streicher, W W, van der Walle, C F, Uddin, S, Winter, G, Roessner, D, Harris, P & Friess, W 2020, ' Advancing Therapeutic Protein Discovery and Development through Comprehensive Computational and Biophysical Characterization ', Molecular Pharmaceutics, vol. 17, no. 2, pp. 426-440 . https://doi.org/10.1021/acs.molpharmaceut.9b00852
Therapeutic protein candidates should exhibit favorable properties that render them suitable to become drugs. Nevertheless, there are no well-established guidelines for the efficient selection of proteinaceous molecules with desired features during e
Autor:
Alina Kulakova, Dierk Roessner, Wolfgang Frieß, Pernille Sønderby, Werner Streicher, Pernille Harris, Lorenzo Gentiluomo, Günther H.J. Peters, Sowmya Indrakumar
Publikováno v:
Journal of Structural Biology: X
Journal of structural biology: X 4, 100017-(2020). doi:10.1016/j.yjsbx.2019.100017
Journal of Structural Biology: X, Vol 4, Iss, Pp 100017-(2020)
Kulakova, A, Indrakumar, S, Sønderby, P, Gentiluomo, L, Streicher, W, Roessner, D, Frieß, W, Peters, G H J & Harris, P 2020, ' Small angle X-ray scattering and molecular dynamic simulations provide molecular insight for stability of recombinant human transferrin ', Journal of Structural Biology: X, vol. 4, 100017 . https://doi.org/10.1016/j.yjsbx.2019.100017
Journal of structural biology: X 4, 100017-(2020). doi:10.1016/j.yjsbx.2019.100017
Journal of Structural Biology: X, Vol 4, Iss, Pp 100017-(2020)
Kulakova, A, Indrakumar, S, Sønderby, P, Gentiluomo, L, Streicher, W, Roessner, D, Frieß, W, Peters, G H J & Harris, P 2020, ' Small angle X-ray scattering and molecular dynamic simulations provide molecular insight for stability of recombinant human transferrin ', Journal of Structural Biology: X, vol. 4, 100017 . https://doi.org/10.1016/j.yjsbx.2019.100017
Graphical abstract
Highlights • Combination of SAXS and MD simulations can explain the molecular background for the stability studies. • Destabilizing excipients in general induce opening of the N-lobe by binding to specific residues in the
Highlights • Combination of SAXS and MD simulations can explain the molecular background for the stability studies. • Destabilizing excipients in general induce opening of the N-lobe by binding to specific residues in the
Publikováno v:
Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-8 (2019)
Scientific Reports, Vol 9, Iss 1, Pp 1-8 (2019)
Multi-angle light scattering coupled with size-exclusion chromatography (SEC-MALS) is a standard approach for protein characterization. Recently MALS detection has been coupled with ion-exchange chromatography (IEX) which demonstrated the feasibility
Autor:
Lorenzo Gentiluomo, Guenther H. H. J. Peters, Alina Kulakova, Pernille Harris, Werner Streicher, Wolfgang Frieß, Pernille Sønderby, Dierk Roessner, Sowmya Indrakumar
Publikováno v:
bioRxiv
Transferrin is an attractive candidate for drug delivery due to its ability to cross the blood brain barrier. However, in order to be able to use it for therapeutic purposes, it is important to investigate how its stability depends on different formu