Protein engineering of amine transaminases

Autor: Qinglong Meng, Carlos Ramírez-Palacios, Hein J. Wijma, Dick B. Janssen

Publikováno v: Frontiers in Catalysis, Vol 2 (2022)

Protein engineering is a powerful and widely applied tool for tailoring enzyme properties to meet application-specific requirements. An attractive group of biocatalysts are PLP-dependent amine transaminases which are capable of converting prochiral k

Externí odkaz: https://doaj.org/article/39999081950b43df80b7f653713a0e58

Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance

Autor: Misun Lee, Henriëtte J. Rozeboom, Eline Keuning, Paul de Waal, Dick B. Janssen

Publikováno v: Biotechnology for Biofuels, Vol 13, Iss 1, Pp 1-18 (2020)

Abstract Background Efficient bioethanol production from hemicellulose feedstocks by Saccharomyces cerevisiae requires xylose utilization. Whereas S. cerevisiae does not metabolize xylose, engineered strains that express xylose isomerase can metaboli

Externí odkaz: https://doaj.org/article/c8ba0ea90cda4e39940ae571e707fd0b

Computationally Supported Inversion of Ketoreductase Stereoselectivity

Autor: Estela Delgado‐Arciniega, Hein J. Wijma, Chantal Hummel, Dick B. Janssen

Publikováno v: ChemBioChem, 24(9):e202300032. WILEY-V C H VERLAG GMBH

Whereas directed evolution and rational design by structural inspection are established tools for enzyme redesign, computational methods are less mature but have the potential to predict small sets of mutants with desired properties without laborator

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6acb66ca1c5fa45cc69f53be5d1a9d30
https://doi.org/10.1002/cbic.202300032

Computational Redesign of an ω-Transaminase from Pseudomonas jessenii for Asymmetric Synthesis of Enantiopure Bulky Amines

Autor: Carlos Ramírez-Palacios, Hein J. Wijma, Siewert J. Marrink, Mattijs E. Hooghwinkel, Nikolas Capra, Henriëtte J. Rozeboom, Qinglong Meng, Sebastian Thallmair, Dick B. Janssen, Andy-Mark W. H. Thunnissen

Publikováno v: ACS Catalysis, 11(17), 10733-10747. AMER CHEMICAL SOC
ACS Catalysis

ω-Transaminases (ω-TA) are attractive biocatalysts for the production of chiral amines from prochiral ketones via asymmetric synthesis. However, the substrate scope of ω-TAs is usually limited due to steric hindrance at the active site pockets. We

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5164e9a483b603ad8fc5a2f3582837fb
https://doi.org/10.1021/acscatal.1c02053

Engineering Thermostability in Artificial Metalloenzymes to Increase Catalytic Activity

Autor: Megan V. Doble, Henriëtte J. Rozeboom, Misun Lee, Paul C. J. Kamer, Lorenz Obrecht, Emma Branigan, Henk-Jan Joosten, James H. Naismith, Amanda G. Jarvis, Dick B. Janssen

Publikováno v: Doble, M V, Obrecht, L, Joosten, H, Lee, M, Rozeboom, H J, Branigan, E, Naismith, J H, Janssen, D B, Jarvis, A G & Kamer, P C J 2021, ' Engineering Thermostability in Artificial Metalloenzymes to Increase Catalytic Activity ', ACS Catalysis, vol. 11, no. 6, pp. 3620-3627 . https://doi.org/10.1021/acscatal.0c05413
ACS Catalysis, 11(6), 3620-3627. AMER CHEMICAL SOC

Protein engineering has shown widespread use in improving the industrial application of enzymes and broadening the conditions they are able to operate under by increasing their thermostability and solvent tolerance. Here, we show that protein enginee

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::680ec28cfab832923c74a3b9fae466ec
https://hdl.handle.net/11370/2eed633d-e6b2-45b7-a61b-adf53bca77f6