Protein‐NMR‐Resonanzzuordnung ohne Spektralanalyse: automatisierte Festkörper‐Projektionsspektroskopie in 5D (SO‐APSY)

Autor: Tanguy Le Marchand, Wolfgang Bermel, Claire Ollier, Jan Stanek, Henry W. Orton, Guido Pintacuda, Isabella C. Felli, Diane Cala de Paepe, Dylan Foucaudeau, Adrian W. Draney, Sebastian Hiller, Tobias Schubeis, Roberta Pierattelli

Publikováno v: Angewandte Chemie. 132:2400-2405

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::946a7b8a99865b77796a7b827824f5d3
https://doi.org/10.1002/ange.201912211

Dynamic Nuclear Polarization-Enhanced Biomolecular NMR Spectroscopy at High Magnetic Field with Fast Magic-Angle Spinning

Autor: Olivier Ouari, Diane Cala de Paepe, Inara Akopjana, Svetlana Kotelovica, G. Pintacuda, Anne Lesage, Andrea Pica, Andrew J. Pell, Kristaps Jaudzems, Andrea Bertarello, Sachin Rama Chaudhari, Emeline Barbet-Massin, Kaspars Tars, David Gajan

Publikováno v: Angewandte Chemie International Edition
Angewandte Chemie International Edition, Wiley-VCH Verlag, 2018, 57 (25), pp.7458-7462. ⟨10.1002/anie.201801016⟩
Angewandte Chemie International Edition, 2018, 57 (25), pp.7458-7462. ⟨10.1002/anie.201801016⟩

International audience; Dynamic nuclear polarization (DNP) is a powerful way to overcome the sensitivity limitation of magic-angle-spinning (MAS) NMR experiments. However, the resolution of the DNPNMR spectra of proteins is compromised by severe line

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cc9e0d62449bc45746e65fcdc1af98e
https://hal.archives-ouvertes.fr/hal-01859534

Deciphering the Role of CD1e Protein in Mycobacterial Phosphatidyl- myo -inositol Mannosides (PIM) Processing for Presentation by CD1b to T Lymphocytes

Autor: Emilie Layre, Diane Cala-De Paepe, Luis F. Garcia-Alles, Lucia Mori, Germain Puzo, Gaëlle Giacometti, Daniel Hanau, Martine Gilleron, Gennaro De Libero

Publikováno v: Journal of Biological Chemistry
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2012, 287 (37), pp.31494-31502. ⟨10.1074/jbc.M112.386300⟩
The Journal of biological chemistry

International audience; Lipids are important antigens that induce T cell-mediated specific immune responses. They are presented to T lymphocytes by a specific class of MHC-I like proteins, termed CD1. The majority of the described CD1-presented mycob

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::428d4b782398e4680f77c76616e7fef8
https://hal.archives-ouvertes.fr/hal-03096337

Is protein deuteration beneficial for proton detected solid-state NMR at and above 100 kHz magic-angle spinning?

Autor: Kristaps Jaudzems, Guido Pintacuda, Loren B. Andreas, Kaspars Tars, Diane Cala de Paepe, Jan Stanek

Publikováno v: Solid State Nuclear Magnetic Resonance
Solid State Nuclear Magnetic Resonance, Elsevier, 2017, 87, pp.126-136. ⟨10.1016/j.ssnmr.2017.07.004⟩
Solid State Nuclear Magnetic Resonance, 2017, 87, pp.126-136. ⟨10.1016/j.ssnmr.2017.07.004⟩

We thank the members of technical staff of ISA for assistance with NMR spectrometers.; International audience; 1H-detection in solid-state NMR of proteins has been traditionally combined with deuteration for both resolution and sensitivity reasons, w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c44cf552d53663dd636cf0323bab00af

Structure of fully protonated proteins by proton-detected magic-angle spinning NMR

Autor: Torsten Herrmann, Guido Pintacuda, Inara Akopjana, Lyndon Emsley, Sebastian Wegner, Andrea Bertarello, Jan Stanek, Daniela Lalli, Loren B. Andreas, Kristaps Jaudzems, Tanguy Le Marchand, Diane Cala de Paepe, Svetlana Kotelovica, Benno Knott, Anne Lesage, Frank Engelke, Kaspars Tars

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2016, 113 (33), pp.9187-9192. ⟨10.1073/pnas.1602248113⟩
Proceedings of the National Academy of Sciences

International audience; Protein structure determination by proton-detected magic-angle spinning (MAS) NMR has focused on highly deuterated samples, in which only a small number of protons are introduced and observation of signals from side chains is

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::439c1fb5ce6afc4adee068c2f68c2355
https://infoscience.epfl.ch/record/222219