Promiscuous splicing-derived hairpins are dominant substrates of tailing-mediated defense of miRNA biogenesis in mammals

Autor: Seungjae Lee, David Jee, Sid Srivastava, Acong Yang, Abhinav Ramidi, Renfu Shang, Diane Bortolamiol-Becet, Sébastien Pfeffer, Shuo Gu, Jiayu Wen, Eric C. Lai

Publikováno v: Cell Reports, Vol 42, Iss 2, Pp 112111- (2023)

Summary: Canonical microRNA (miRNA) hairpins are processed by the RNase III enzymes Drosha and Dicer into ∼22 nt RNAs loaded into an Argonaute (Ago) effector. In addition, splicing generates numerous intronic hairpins that bypass Drosha (mirtrons)

Externí odkaz: https://doaj.org/article/e5a6e87b637141948837c14c388a3164

Importance of miRNA stability and alternative primary miRNA isoforms in gene regulation during Drosophila development

Autor: Greg Tucker-Kellogg, Diane Bortolamiol-Becet, Katsutomo Okamura, Nicholas S. Tolwinski, Mandy Yu Theng Lim, Prameet Kaur, Li Zhou, Vikneswaran Gopal, Abil Saj

Publikováno v: eLife, Vol 7 (2018)

Mature microRNAs (miRNAs) are processed from primary transcripts (pri-miRNAs), and their expression is controlled at transcriptional and post-transcriptional levels. However, how regulation at multiple levels achieves precise control remains elusive.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::172052a69d40f890062848969e3b1f03
https://doi.org/10.7554/elife.38389

Adaptive Regulation of Testis Gene Expression and Control of Male Fertility by the Drosophila Hairpin RNA Pathway

Autor: Raquel Martin, Julie A. Brill, Hong Duan, Fernando Bejarano, J. Graham Ruby, Eric C. Lai, Lacramioara Fabian, Jiayu Wen, Diane Bortolamiol-Becet, Katsutomo Okamura

Publikováno v: Molecular Cell. 57:165-178

Although endogenous siRNAs (endo-siRNAs) have been described in many species, still little is known about their endogenous utility. Here, we show that Drosophila hairpin RNAs (hpRNAs) generate an endo-siRNA class with predominant expression in testes

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e62d40f460a402fddc5e44242e04e3d
https://doi.org/10.1016/j.molcel.2014.11.025

Diversity of miRNAs, siRNAs, and piRNAs across 25 Drosophila cell lines

Autor: Jaaved Mohammed, Lucy Cherbas, Jakub Orzechowski Westholm, Peter Cherbas, Harrison Tsai, Thomas C. Kaufman, Jiayu Wen, Qi Dai, Diane Bortolamiol-Becet, Dayu Zhang, Katsutomo Okamura, Nicolas Robine, Erik Ladewig, Justen Andrews, Alex S. Flynt, Eric C. Lai, Adam Siepel

Publikováno v: Genome Research. 24:1236-1250

We expanded the knowledge base for Drosophila cell line transcriptomes by deeply sequencing their small RNAs. In total, we analyzed more than 1 billion raw reads from 53 libraries across 25 cell lines. We verify reproducibility of biological replicat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4ae322c3b65c32a6a5d49ddae7e5bac
https://doi.org/10.1101/gr.161554.113

Deep annotation of Drosophila melanogaster microRNAs yields insights into their processing, modification, and emergence

Autor: Nicolas Robine, Marco A. Marra, Norbert Perrimon, Diane Bortolamiol-Becet, Gregory J. Hannon, Anastasia Samsonova, Raquel Martin, Ammar S. Naqvi, Yongjun Zhao, Katsutomo Okamura, Zhiping Weng, Jui-Hung Hung, Jakub Orzechowski Westholm, Phillip D. Zamore, Qi Dai, Eric C. Lai, Eugene Berezikov

Publikováno v: Genome Research, 21(2), 203-215. COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
Genome Research, 21(2), 203-215. Cold Spring Harbor Laboratory Press

Since the initial annotation of miRNAs from cloned short RNAs by the Ambros, Tuschl, and Bartel groups in 2001, more than a hundred studies have sought to identify additional miRNAs in various species. We report here a meta-analysis of short RNA data

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77994190bb7bdcf64d541d8b1e52700e
https://doi.org/10.1101/gr.116657.110