Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Diane Biegel"'
Publikováno v:
The Journal of Infectious Diseases. 183:697-706
These studies demonstrate that in vitro stimulation of spleen cells from murine cytomegalovirus (MCMV) immune mice with MCMV-infected fibroblasts induces production of interferon (IFN)-gamma. This response is specific to MCMV, is not generalized to h
Publikováno v:
Journal of Virological Methods. 58:121-129
A rapid and sensitive radioimmunoassay for the quantitation of HCMV binding and infection of human fibroblasts (HFF) was developed. The protocol involves the use of a monoclonal antibody (27-156) reactive with HCMV gB (alpha-gB), followed by an 125I-
Autor:
Diane Biegel, Joel S. Pachter
Publikováno v:
Neuroscience Research Communications. 18:47-55
Monocytic and astroglial cells interact in culture to cause the release of several neuroactive compounds. The mechanism by which this occurs is unknown, though adhesion between these two cell types has been suggested as a possible triggering event. I
Autor:
Diane Biegel, Joel S. Pachter
Publikováno v:
In Vitro Cellular & Developmental Biology - Animal. 30:581-588
Brain microvessel endothelial cells (BMEC) exhibit the tendency to migrate through 3.0-vm pore semipermeable inserts and establish monolayers on both apical and basal filter surfaces. This can potentially lead to complications in accurately assessing
Publikováno v:
European Journal of Biochemistry. 212:217-225
Association of mRNA with the cytoskeletal framework (CSK) is thought to play a strategic role in the placement of mRNA in the cytoplasm. However, the molecular determinants underlying mRNA/CSK association are completely unknown. To begin addressing t
Autor:
Diane Biegel, Joel S. Pachter
Publikováno v:
In Vitro Cellular & Developmental Biology - Animal. 27:75-85
We have developed a novel, "in situ" translation system derived from cultured cells that are subject to mild detergent extraction. By using a low concentration of nonionic detergent to gently permeabilize cells while they remain adherent to a substra
Autor:
Diane Biegel, Thomas R. Kosten
Publikováno v:
Expert review of vaccines. 1(3)
Immunotherapies are under development as a new approach to the treatment of substance dependence. The drugs of abuse currently being tested using this new approach are nicotine, cocaine, phencyclidine and methamphetamine. In laboratory animal models,
Publikováno v:
Brain research. 692(1-2)
A simplified protocol for isolating brain microvessel endothelial cells (BMEC) from human cortex and culturing them on a thick collagen plug is described. This method results in the establishment of monolayers of BMEC that retain numerous properties
Autor:
Diane Biegel, Joel S. Pachter
Publikováno v:
Journal of cellular biochemistry. 48(1)
A multitude of studies has indicated that the vast majority of mRNA and polyribosomes is associated with the detergent-resistant cytoskeletal faramework (CSK). However, the nature and purpose of this association remain unclear. To begin unraveling th
Autor:
Alexandros Makriyannis, Diane Biegel
Navigate the complex and multidisciplinary path of drug discovery procedures with Drug Discovery Strategies and Methods-a well-organized and timely reference that analyzes methods in target identification and validation, lead detection, compound opti