Zobrazeno 1 - 10
of 72
pro vyhledávání: '"Diane, Hatton"'
Autor:
Ying Bai, Ivan Domenech Mercadé, Ramy Elgendy, Giulia Lambiase, Sew Peak-Chew, Catarina Franco, Steven W. Wingett, Tim J. Stevens, Luigi Grassi, Noah Hitchcock, Cristina Sayago Ferreira, Diane Hatton, Elizabeth A. Miller, Rajesh K. Mistry
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 27, Iss , Pp 17-31 (2025)
The secretory capacity of Chinese hamster ovary (CHO) cells remains a fundamental bottleneck in the manufacturing of protein-based therapeutics. Unconventional biological drugs with complex structures and processing requirements are particularly prob
Externí odkaz:
https://doaj.org/article/808e407dcb674f72af2e309420f393fc
Publikováno v:
mAbs, Vol 15, Iss 1 (2023)
ABSTRACTIntrons are included in genes encoding therapeutic proteins for their well-documented function of boosting expression. However, mis-splicing of introns in recombinant immunoglobulin (IgG) heavy chain (HC) transcripts can produce amino acid se
Externí odkaz:
https://doaj.org/article/ab876963243a4450ba80756faf129466
Autor:
Stephen Goldrick, Haneen Alosert, Clare Lovelady, Nicholas J. Bond, Tarik Senussi, Diane Hatton, John Klein, Matthew Cheeks, Richard Turner, James Savery, Suzanne S. Farid
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 11 (2023)
Cell line development is an essential stage in biopharmaceutical development that often lies on the critical path. Failure to fully characterise the lead clone during initial screening can lead to lengthy project delays during scale-up, which can pot
Externí odkaz:
https://doaj.org/article/b8b9e6ce0bbe496d96efa8a7b7ef2a67
Autor:
Lina Chakrabarti, Raghothama Chaerkady, Junmin Wang, Shao Huan Samuel Weng, Chunlei Wang, Chen Qian, Lisa Cazares, Sonja Hess, Peter Amaya, Jie Zhu, Diane Hatton
Publikováno v:
mAbs, Vol 14, Iss 1 (2022)
With the aim of increasing protein productivity of Chinese hamster ovary (CHO) cells, we sought to generate new CHO hosts with favorable biomanufacturing phenotypes and improved functionality. Here, we present an innovative approach of enriching the
Externí odkaz:
https://doaj.org/article/87d336fa83474b2f8e0099c9ea20336b
Autor:
Rasool Saghaleyni, Magdalena Malm, Noah Moruzzi, Jan Zrimec, Ronia Razavi, Num Wistbacka, Hannes Thorell, Anton Pintar, Andreas Hober, Fredrik Edfors, Veronique Chotteau, Per-Olof Berggren, Luigi Grassi, Aleksej Zelezniak, Thomas Svensson, Diane Hatton, Jens Nielsen, Jonathan L. Robinson, Johan Rockberg
Publikováno v:
Cell Reports, Vol 39, Iss 11, Pp 110936- (2022)
Summary: Recombinant protein production can cause severe stress on cellular metabolism, resulting in limited titer and product quality. To investigate cellular and metabolic characteristics associated with these limitations, we compare HEK293 clones
Externí odkaz:
https://doaj.org/article/039c0d28027d41c49243a52c9ff39b2d
Autor:
Caijuan Zhan, Gholamreza Bidkhori, Hubert Schwarz, Magdalena Malm, Aman Mebrahtu, Ray Field, Christopher Sellick, Diane Hatton, Paul Varley, Adil Mardinoglu, Johan Rockberg, Veronique Chotteau
Publikováno v:
iScience, Vol 23, Iss 11, Pp 101653- (2020)
Summary: Human embryonic kidney cells HEK293 can be used for the production of therapeutic glycoproteins requiring human post-translational modifications. High cell density perfusion processes are advantageous for such production but are challenging
Externí odkaz:
https://doaj.org/article/3527cbee461a49d0a4b65321f003c3e5
Autor:
Magdalena Malm, Rasool Saghaleyni, Magnus Lundqvist, Marco Giudici, Veronique Chotteau, Ray Field, Paul G. Varley, Diane Hatton, Luigi Grassi, Thomas Svensson, Jens Nielsen, Johan Rockberg
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-2 (2021)
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Externí odkaz:
https://doaj.org/article/9b25893b02334dbda85e6f0d5d50fee7
Autor:
M. Moradi Barzadd, Anna-Luisa Volk, Diane Hatton, Raymond Field, Aman Mebrahtu, Paul Varley, R. G. Roth, Veronique Chotteau, N. Wistabacka, Fredrik Edfors, Chih-Chung Kuo, Magdalena Malm, Nathan E. Lewis, Ronia Razavi, T. Graslund, Johan Rockberg, David Kotol, M. Lunqvist
Publikováno v:
Metabolic Engineering. 72:171-187
Biologics represent the fastest growing group of therapeutics, but many advanced recombinant protein moieties remain difficult to produce. Here, we identify metabolic engineering targets limiting expression of recombinant human proteins through a sys
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc0046abd38c8f4d0e6970b315d289d7
https://doi.org/10.1016/j.ymben.2022.03.009
https://doi.org/10.1016/j.ymben.2022.03.009
Autor:
Mona Moradi Barzadd, Magnus Lundqvist, Claire Harris, Magdalena Malm, Anna-Luisa Volk, Niklas Thalén, Veronique Chotteau, Luigi Grassi, Andrew Smith, Marina Leal Abadi, Giulia Lambiase, Suzanne Gibson, Diane Hatton, Johan Rockberg
Publikováno v:
New Biotechnology. 68:68-76
Aggregation of therapeutic bispecific antibodies negatively affects the yield, shelf-life, efficacy and safety of these products. Pairs of stable Chinese hamster ovary (CHO) cell lines produced two difficult-to-express bispecific antibodies with diff
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7301a07239db35adb559b35c715b648
https://doi.org/10.1016/j.nbt.2022.01.010
https://doi.org/10.1016/j.nbt.2022.01.010
Autor:
Si Nga Sou, Claire L. Harris, Rebecca Williams, Dorota Kozub, Fabio Zurlo, Yash D. Patel, Praveen Kallamvalli Illam Sankaran, Olalekan Daramola, Adam Brown, David C. James, Diane Hatton, Sarah Dunn, Suzanne J. Gibson
Publikováno v:
Biotechnology Progress.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::088b3dd34516a35eafaed5112c57e28d
https://doi.org/10.1002/btpr.3348
https://doi.org/10.1002/btpr.3348