Shotgun transcriptome, spatial omics, and isothermal profiling of SARS-CoV-2 infection reveals unique host responses, viral diversification, and drug interactions

Autor: Daniel Butler, Christopher Mozsary, Cem Meydan, Jonathan Foox, Joel Rosiene, Alon Shaiber, David Danko, Ebrahim Afshinnekoo, Matthew MacKay, Fritz J. Sedlazeck, Nikolay A. Ivanov, Maria Sierra, Diana Pohle, Michael Zietz, Undina Gisladottir, Vijendra Ramlall, Evan T. Sholle, Edward J. Schenck, Craig D. Westover, Ciaran Hassan, Krista Ryon, Benjamin Young, Chandrima Bhattacharya, Dianna L. Ng, Andrea C. Granados, Yale A. Santos, Venice Servellita, Scot Federman, Phyllis Ruggiero, Arkarachai Fungtammasan, Chen-Shan Chin, Nathaniel M. Pearson, Bradley W. Langhorst, Nathan A. Tanner, Youngmi Kim, Jason W. Reeves, Tyler D. Hether, Sarah E. Warren, Michael Bailey, Justyna Gawrys, Dmitry Meleshko, Dong Xu, Mara Couto-Rodriguez, Dorottya Nagy-Szakal, Joseph Barrows, Heather Wells, Niamh B. O’Hara, Jeffrey A. Rosenfeld, Ying Chen, Peter A. D. Steel, Amos J. Shemesh, Jenny Xiang, Jean Thierry-Mieg, Danielle Thierry-Mieg, Angelika Iftner, Daniela Bezdan, Elizabeth Sanchez, Thomas R. Campion, John Sipley, Lin Cong, Arryn Craney, Priya Velu, Ari M. Melnick, Sagi Shapira, Iman Hajirasouliha, Alain Borczuk, Thomas Iftner, Mirella Salvatore, Massimo Loda, Lars F. Westblade, Melissa Cushing, Shixiu Wu, Shawn Levy, Charles Chiu, Robert E. Schwartz, Nicholas Tatonetti, Hanna Rennert, Marcin Imielinski, Christopher E. Mason

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)

Here, using clinical samples and autopsy tissues, the authors combine fast-colorimetric test (LAMP) for SARS-CoV-2 infection and large-scale shotgun metatranscriptomics for host, viral, and microbial profiling and provide a map of the viral genetic f

Externí odkaz: https://doaj.org/article/e049d27579f84e2b9f12b7b11bc7c436

Shotgun transcriptome, spatial omics, and isothermal profiling of SARS-CoV-2 infection reveals unique host responses, viral diversification, and drug interactions

Autor: Priya Velu, David Danko, Alain C. Borczuk, Michael Bailey, Daniela Bezdan, Craig Westover, Charles Y. Chiu, Evan Sholle, Tyler Hether, Peter A D Steel, Dorottya Nagy-Szakal, Yale A. Santos, Justyna Gawrys, Jeffrey A. Rosenfeld, Krista Ryon, Fritz J. Sedlazeck, Vijendra Ramlall, Amos J Shemesh, Cem Meydan, Shawn Levy, Angelika Iftner, Undina Gisladottir, Chandrima Bhattacharya, Robert E. Schwartz, Venice Servellita, Dianna Ng, Nikolay A. Ivanov, Massimo Loda, Arkarachai Fungtammasan, Jean Thierry-Mieg, Lars F. Westblade, Ying Chen, Joel Rosiene, Marcin Imielinski, Ebrahim Afshinnekoo, Joseph E. Barrows, Matthew MacKay, Chen-Shan Chin, Daniel Butler, Dong Xu, Sarah Warren, Jonathan Foox, Ciaran Hassan, Heather L. Wells, Andrea Granados, Lin Cong, Thomas R. Campion, Ari Melnick, Alon Shaiber, John Sipley, Sagi Shapira, Jason Reeves, Elizabeth Sanchez, Christopher Mozsary, Melissa M. Cushing, Thomas Iftner, Arryn Craney, Iman Hajirasouliha, Maria A. Sierra, Youngmi Kim, Scot Federman, Nathan A. Tanner, Niamh B. O’Hara, Christopher E. Mason, Hanna Rennert, Edward J. Schenck, Nicholas P. Tatonetti, Mirella Salvatore, Mara Couto-Rodriguez, Nathaniel M. Pearson, Benjamin Young, Michael Zietz, Shixiu Wu, Dmitry Meleshko, Jenny Xiang, Bradley W. Langhorst, P. Ruggiero, Danielle Thierry-Mieg, Diana Pohle

Publikováno v: Nature communications, vol 12, iss 1
Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
bioRxiv

In less than nine months, the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) killed over a million people, including >25,000 in New York City (NYC) alone. The COVID-19 pandemic caused by SARS-CoV-2 highlights clinical needs to detect in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2a1904c1d0f41fac063d7b8ad05410d
https://escholarship.org/uc/item/41c882p6

Adenoviral transduction supports matrix expression of alginate cultured articular chondrocytes

Autor: Diana Pohle, R. Kasch, Thomas Mittlmeier, Rainer Bader, Brigitte Müller-Hilke, Philipp Herlyn, B.M. Pützer

Publikováno v: Biotechnology and Bioengineering. 109:2402-2408

The present study examines the effects of adenoviral (Ad) transduction of human primary chondrocyte on transgene expression and matrix production. Primary chondrocytes were isolated from healthy articular cartilage and from cartilage with mild osteoa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ab2b670a5143c7549dd425ce554dfdc
https://doi.org/10.1002/bit.24505