Sortase-Mediated Ligation of Purely Artificial Building Blocks

Autor: Xiaolin Dai, Diana M. Mate, Ulrich Glebe, Tayebeh Mirzaei Garakani, Andrea Körner, Ulrich Schwaneberg, Alexander Böker

Publikováno v: Polymers, Vol 10, Iss 2, p 151 (2018)

Sortase A (SrtA) from Staphylococcus aureus has been often used for ligating a protein with other natural or synthetic compounds in recent years. Here we show that SrtA-mediated ligation (SML) is universally applicable for the linkage of two purely a

Externí odkaz: https://doaj.org/article/11dc3fdf2c0d4afa8d615f95ffa093dc

Thermostability engineering of a class ii pyruvate aldolase from Escherichia coli by in vivo folding interference

Autor: Diana M. Mate, Pere Clapés, Esther Sanchez-Freire, Đurđa Vasić-Rački, Yuyin Qi, Karel Hernández, Morana Česnik, Zvjezdana Findrik Blažević, José Berenguer, María Luisa del Pozo, Aurelio Hidalgo, Sandra Bosch, Jaime Quesada

Publikováno v: Biblos-e Archivo. Repositorio Institucional de la UAM
Consejo Superior de Investigaciones Científicas (CSIC)
Digital.CSIC. Repositorio Institucional del CSIC
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The use of enzymes in industrial processes is often limited by the unavailability of biocatalysts with prolonged stability. Thermostable enzymes allow increased process temperature and thus higher substrate and product solubility, reuse of expensive

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5655a8bfdeec6e4839d90bf98389848a
http://hdl.handle.net/10486/702405

Directed sortase A evolution for efficient site-specific bioconjugations in organic co-solvents

Autor: Ulrich Schwaneberg, Hoda Alibiglou, Felix Jakob, Zhi Zou, Diana M. Mate, Mehdi D. Davari

Publikováno v: Chemical Communications. 54:11467-11470

Directed sortase A evolution yielded the variants R159G and D165Q/D186G/K196V with increased resistance (2.2-fold) and catalytic efficiency (6.3-fold) in 45% (v/v) dimethylsulfoxide. Interestingly, D165Q/D186G/K196V also showed an up to 4.7-fold incr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::365299c0aff65aa06f1929735ecfe87d
https://doi.org/10.1039/c8cc06017g

Thermostability enhancement of the Pseudomonas fluorescens esterase I by in vivo folding selection in Thermus thermophilus

Autor: Noé R Rivera, Juan A. Ayala, Aurelio Hidalgo, José Berenguer, Diana M. Mate, Esther Sanchez-Freire

Publikováno v: Biotechnology and bioengineering. 117(1)

Prolonged stability is a desired property for the biotechnological application of enzymes since it allows its reutilization, contributing to making biocatalytic processes more economically competitive with respect to chemical synthesis. In this study

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f196f5dc95e1d11a5afd00f41ef397d0
https://pubmed.ncbi.nlm.nih.gov/31529702

Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization

Autor: Felix Jakob, Zhi Zou, Maximilian Nöth, Diana M. Mate, Ulrich Schwaneberg

Publikováno v: Chemistry (Weinheim an Der Bergstrasse, Germany)
Digital.CSIC: Repositorio Institucional del CSIC
Consejo Superior de Investigaciones Científicas (CSIC)
Digital.CSIC. Repositorio Institucional del CSIC
instname
Chemistry-a European journal 26(60), 13568-13572 (2020). doi:10.1002/chem.202002740

Staphylococcus aureus sortase A (SaSrtA) is widely used for site‐specific protein modifications, but it lacks the robustness for performing bioconjugation reactions at elevated temperatures or in presence of denaturing agents. Loop engineering and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab7453b2d127bb9020fb6a97a1c8af63
https://doi.org/10.1002/chem.202004039