Heterologous production of fungal secondary metabolites in Aspergilli

Autor: Diana Chinyere Anyaogu, Uffe Hasbro Mortensen

Publikováno v: Frontiers in Microbiology, Vol 6 (2015)

Fungal natural products comprise a wide range of compounds. Some are medically attractive as drugs and drug leads, some are used as food additives, while others are harmful mycotoxins. In recent years the genome sequence of several fungi has become a

Externí odkaz: https://doaj.org/article/a5fb637662354a94acb76f74185a4e95

Heterologous reconstitution of the intact geodin gene cluster in Aspergillus nidulans through a simple and versatile PCR based approach.

Autor: Morten Thrane Nielsen, Jakob Blæsbjerg Nielsen, Diana Chinyere Anyaogu, Dorte Koefoed Holm, Kristian Fog Nielsen, Thomas Ostenfeld Larsen, Uffe Hasbro Mortensen

Publikováno v: PLoS ONE, Vol 8, Iss 8, p e72871 (2013)

Fungal natural products are a rich resource for bioactive molecules. To fully exploit this potential it is necessary to link genes to metabolites. Genetic information for numerous putative biosynthetic pathways has become available in recent years th

Externí odkaz: https://doaj.org/article/52f4152da0a24051bb761a4448e348dc

Glycoengineering of Aspergillus nidulans to produce precursors for humanized N-glycan structures

Autor: Hui Zhang, Shuang Yang, Jackson T. Paul, Timothy John Hobley, Fabiano Jares Contesini, Diana Chinyere Anyaogu, Kristian Fog Nielsen, Natalia I. Majewska, Uffe Hasbro Mortensen, Anders Holmgaard Hansen, Michael J. Betenbaugh, Jakob Blæsbjerg Hoof

Publikováno v: Anyaogu, D C, Hansen, A H, Hoof, J B, Majewska, N I, Contesini, F J, Paul, J T, Nielsen, K F, Hobley, T J, Yang, S, Zhang, H, Betenbaugh, M & Mortensen, U H 2021, ' Glycoengineering of Aspergillus nidulans to produce precursors for humanized N-glycan structures ', Metabolic Engineering, vol. 67, pp. 153-163 . https://doi.org/10.1016/j.ymben.2021.06.001

Filamentous fungi secrete protein with a very high efficiency, and this potential can be exploited advantageously to produce therapeutic proteins at low costs. A significant barrier to this goal is posed by the fact that fungal N-glycosylation varies

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b388308c73fcc3646e8af31fda80f23a
https://orbit.dtu.dk/en/publications/ec2d1277-9672-41af-9974-415042cf67fd

Effects of dissolved oxygen concentration and iron addition on immediate-early gene expression of Magnetospirillum gryphiswaldense MSR-1

Autor: Takeshi Kasama, Uffe Hasbro Mortensen, Mhairi Workman, Diana Chinyere Anyaogu, Shiwen Zhuang, Timothy John Hobley

Publikováno v: Zhuang, S, Anyaogu, D C, Kasama, T, Workman, M, Mortensen, U H & Hobley, T J 2017, ' Effects of Dissolved Oxygen Concentration and Iron Addition on Immediate-early Gene Expression of Magnetospirillum gryphiswaldense MSR-1 ', FEMS Microbiology Letters, vol. 364, no. 11, fnx079 . https://doi.org/10.1093/femsle/fnx079

We report the effects of dissolved oxygen (DO) concentration and iron addition on gene expression of Magnetospirillum gryphiswaldense MSR-1 cells during fermentations, focusing on 0.25-24 h after iron addition. The DO was strictly controlled at 0.5%

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba7ed3dd99f1781a340edc1998fcaa63
https://pubmed.ncbi.nlm.nih.gov/28430950

Effect of secretory pathway gene overexpression on secretion of a fluorescent reporter protein in

Autor: Martin, Schalén, Diana Chinyere, Anyaogu, Jakob Blæsbjerg, Hoof, Mhairi, Workman

Publikováno v: Fungal Biology and Biotechnology

Background The considerable capacity of filamentous fungi for the secretion of proteins is the basis for multi-billion dollar industries producing enzymes and proteins with therapeutic value. The stepwise pathway from translation to secretion is ther

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c1d4dd85b3554f3b8a36a3d2abd5e760
https://pubmed.ncbi.nlm.nih.gov/28955462