Zobrazeno 1 - 6 of 6 pro vyhledávání: '"Diana C. Knapp"'
1
Synthesis of 3′-O-(2-cyanoethyl)-2′-deoxythymidine-5′-phosphate as a model compound for evaluation of cyanoethyl cleavage
Autor: Angelika Keller, Joachim W. Engels, Saulius Serva, Marek Kwiatkowski, Diana C. Knapp
Publikováno v: Collection of Czechoslovak Chemical Communications. 74:515-534
An essential and challenging task during the development of our sequencing-by-synthesis (SBS) technique is the evaluation of efficient cyanoethyl (CE) cleavage conditions. For this purpose 3′-O-(2-cyanoethyl)-2′-deoxythymidine-5′-phosphate as a
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::07e7836df30890ba00de5bbdc1021d71
https://doi.org/10.1135/cccc2008183
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2
The Fluoride Cleavable 2-(Cyanoethoxy)Methyl (CEM) Group as Reversible 3′-O-Terminator for DNA Sequencing-by-Synthesis—Synthesis, Incorporation, and Cleavage
Autor: Marek Kwiatkowski, A. Stura, Joachim W. Engels, S. Zigmantas, Angelika Keller, Diana C. Knapp, A. Földesi
Publikováno v: Nucleosides, Nucleotides and Nucleic Acids. 26:271-275
A new and promising sequencing technology called sequencing-by-synthesis (SBS) enables fast determination of DNA sequences. 2'-Deoxynucleotides containing the (2-cyanoethoxy)methyl (CEM) group at the 3'-O-position are potential reversible terminators
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48c4c4bd8e670427257e120262532885
https://doi.org/10.1080/15257770701257301
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3
Fluorescent labeling of (oligo)nucleotides by a new fluoride cleavable linker capable of versatile attachment modes
Autor: Joachim W. Engels, Diana C. Knapp, Jennifer D’Onofrio
Publikováno v: Bioconjugate chemistry. 21(6)
The development of a fluoride cleavable linker 1 for reversibly labeling (oligo)nucleotides is described here. The linker allows different ways of chemical attachment of a reporter molecule, for example, click chemistry or amide formation. The versat
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a657bb2f7ce264a81a4b4678d2e79fd0
https://pubmed.ncbi.nlm.nih.gov/20509599
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4
ChemInform Abstract: Synthesis of 3′-O-(2-Cyanoethyl)-2′-deoxythymidine-5′-phosphate as a Model Compound for Evaluation of Cyanoethyl Cleavage
Autor: Saulius Serva, Joachim W. Engels, Diana C. Knapp, Marek Kwiatkowski, Angelika Keller
Publikováno v: ChemInform. 40
An essential and challenging task during the development of our sequencing-by-synthesis (SBS) technique is the evaluation of efficient cyanoethyl (CE) cleavage conditions. For this purpose 3′-O-(2-cyanoethyl)-2′-deoxythymidine-5′-phosphate as a
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ea87884dc7e2372e23a1cb4f25f121a3
https://doi.org/10.1002/chin.200933204
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6
Synthesis of four colors fluorescently labelled 3'-O-blocked nucleotides with fluoride cleavable blocking group and linker for array based Sequencing-by-Synthesis applications
Autor: Diana C. Knapp, Maido Remm, Joachim W. Engels, Marek Kwiatkowski, Ants Kurg, Arvydas Lubys, Saulius Serva, Jennifer D’Onofrio, Angelika Keller
Publikováno v: Scopus-Elsevier
Reversible terminators having a fluoride cleavable 3'-O-blocking group are presented. Each nucleotide triphosphate is labelled by a fluorescent dye cleavable by the same reagent. We present here their synthesis, cleavage experiments and polymerase in
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2da7cac79d1137b7f198863b987de14
http://www.scopus.com/inward/record.url?eid=2-s2.0-78649421103&partnerID=MN8TOARS
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