Zobrazeno 1 - 10 of 15 pro vyhledávání: '"Dian, Spakman"'
1
Akademický článek
PICH acts as a force-dependent nucleosome remodeler
Autor: Dian Spakman, Tinka V. M. Clement, Andreas S. Biebricher, Graeme A. King, Manika I. Singh, Ian D. Hickson, Erwin J. G. Peterman, Gijs J. L. Wuite
Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-12 (2022)
In anaphase, any unresolved DNA entanglements between the segregating sister chromatids can give rise to chromatin bridges. Here, the authors present an in vitro single-molecule assay that mimics chromatin under tension, to show that PICH is a tensio
Externí odkaz: https://doaj.org/article/a446e33a0b39439f9380f36b5bf5fe55
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2
Akademický článek
Cellular dynamics of the SecA ATPase at the single molecule level
Autor: Anne-Bart Seinen, Dian Spakman, Antoine M. van Oijen, Arnold J. M. Driessen
Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
Abstract In bacteria, the SecA ATPase provides the driving force for protein secretion via the SecYEG translocon. While the dynamic interplay between SecA and SecYEG in translocation is widely appreciated, it is not clear how SecA associates with the
Externí odkaz: https://doaj.org/article/1c394121d58f465a8a690efb6cc8ef7e
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3
Akademický článek
Method for immobilization of living and synthetic cells for high-resolution imaging and single-particle tracking
Autor: Łukasz Syga, Dian Spakman, Christiaan M. Punter, Bert Poolman
Publikováno v: Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
Abstract Super-resolution imaging and single-particle tracking require cells to be immobile as any movement reduces the resolution of the measurements. Here, we present a method based on APTES-glutaraldehyde coating of glass surfaces to immobilize ce
Externí odkaz: https://doaj.org/article/ad83b3b67e6f4a94848e1347944bf434
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4
Akademický článek
Steric exclusion and protein conformation determine the localization of plasma membrane transporters
Autor: Frans Bianchi, Łukasz Syga, Gemma Moiset, Dian Spakman, Paul E. Schavemaker, Christiaan M. Punter, Anne-Bart Seinen, Antoine M. van Oijen, Andrew Robinson, Bert Poolman
Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-13 (2018)
The yeast plasma membrane consists of membrane microdomains with distinct protein composition. Here the authors use high-resolution single molecule imaging to observe diffusion of specific transmembrane proteins in and out of these microdomains, and
Externí odkaz: https://doaj.org/article/7253120134e54dd39e742760f843a243
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5
Generating Negatively Supercoiled DNA Using Dual-Trap Optical Tweezers
Autor: Graeme A, King, Dian, Spakman, Erwin J G, Peterman, Gijs J L, Wuite
Publikováno v: Methods in molecular biology (Clifton, N.J.). 2478
Many genomic processes lead to the formation of underwound (negatively supercoiled) or overwound (positively supercoiled) DNA. These DNA topological changes regulate the interactions of DNA-binding proteins, including transcription factors, architect
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::7c62506a00bf9a373c97f1a9a69559fd
https://pubmed.ncbi.nlm.nih.gov/36063323
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6
Unravelling the mechanisms of Type 1A topoisomerases using single-molecule approaches
Autor: Andreas S. Biebricher, Gijs J.L. Wuite, Graeme A. King, Dian Spakman, Erwin J.G. Peterman, Julia A M Bakx
Publikováno v: Nucleic Acids Research
Spakman, D, Bakx, J A M, Biebricher, A S, Peterman, E J G, Wuite, G J L & King, G A 2021, ' Unravelling the mechanisms of Type 1A topoisomerases using single-molecule approaches ', Nucleic acids research, vol. 49, no. 10, pp. 5470-5492 . https://doi.org/10.1093/nar/gkab239
Topoisomerases are essential enzymes that regulate DNA topology. Type 1A family topoisomerases are found in nearly all living organisms and are unique in that they require single-stranded (ss)DNA for activity. These enzymes are vital for maintaining
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb0c475cf9cf649e394fdabf2cef2ebe
http://europepmc.org/articles/PMC8191776
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7
Constructing arrays of nucleosome positioning sequences using Gibson Assembly for single-molecule studies
Autor: Gijs J.L. Wuite, Erwin J.G. Peterman, Graeme A. King, Dian Spakman
Publikováno v: Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
Scientific Reports
Spakman, D, King, G A, Peterman, E J G & Wuite, G J L 2020, ' Constructing arrays of nucleosome positioning sequences using Gibson Assembly for single-molecule studies ', Scientific Reports, vol. 10, no. 1, 9903, pp. 1-11 . https://doi.org/10.1038/s41598-020-66259-4
Scientific Reports, 10(1):9903, 1-11. Nature Publishing Group
As the basic building blocks of chromatin, nucleosomes play a key role in dictating the accessibility of the eukaryotic genome. Consequently, nucleosomes are involved in essential genomic transactions such as DNA transcription, replication and repair
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fdcce6a4c54b9bfcc2f09e8befda0a08
http://link.springer.com/article/10.1038/s41598-020-66259-4
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10
Cellular dynamics of the SecA ATPase at the single molecule level
Autor: Antoine M. van Oijen, Anne Bart Seinen, Arnold J. M. Driessen, Dian Spakman
Publikováno v: Scientific Reports, 11(1):1433. Nature Publishing Group
Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
In bacteria, the SecA ATPase provides the driving force for protein secretion via the SecYEG translocon. While the dynamic interplay between SecA and SecYEG in translocation is widely appreciated, it is not clear how SecA associates with the transloc
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d30bebd24f48d613ba1f29cd08dd0283
https://doi.org/10.1038/s41598-021-81081-2
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