Signature morpho-electric properties of diverse GABAergic interneurons in the human neocortex

Autor: Brian Lee, Rachel Dalley, Jeremy A Miller, Thomas Chartrand, Jennie Close, Rusty Mann, Alice Mukora, Lindsay Ng, Lauren Alfiler, Katherine Baker, Darren Bertagnolli, Krissy Brouner, Tamara Casper, Eva Csajbok, Nick Dee, Nicholas Donadio, Stan L.W. Driessens, Tom Egdorf, Rachel Enstrom, Anna A Galakhova, Amanda Gary, Emily Gelfand, Jeff Goldy, Kristen Hadley, Tim S. Heistek, Dijon Hill, Nelson Johansen, Nik Jorstad, Lisa Kim, Agnes Katalin Kocsis, Lauren Kruse, Michael Kunst, Gabriela Leon, Brian Long, Matthew Mallory, Michelle Maxwell, Medea McGraw, Delissa McMillen, Erica J Melief, Gabor Molnar, Marty T Mortrud, Dakota Newman, Julie Nyhus, Ximena Opitz-Araya, Trangthanh Pham, Alice Pom, Lydia Potekhina, Ram Rajanbabu, Augustin Ruiz, Susan M Sunkin, Ildiko Szots, Naz Taskin, Bargavi Thyagarajan, Michael Tieu, Jessica Trinh, Sara Vargas, David Vumbaco, Femke Waleboer, Natalie Weed, Grace Williams, Julia Wilson, Shenqin Yao, Thomas Zhou, Pal Barzo, Trygve Bakken, Charles Cobbs, Richard G. Ellenbogen, Luke Esposito, Manuel Ferreira, Nathan W Gouwens, Benjamin Grannan, Ryder P. Gwinn, Jason S. Hauptman, Rebecca Hodge, Tim Jarsky, C.Dirk Keene, Andrew L. Ko, Boaz Levi, Jeffrey G. Ojemann, Anoop Patel, Jacob Ruzevick, Daniel L. Silbergeld, Kim Smith, Jack Waters, Hongkui Zeng, Jim Berg, Natalia A. Goriounova, Brian Kalmbach, Christiaan P.J. de Kock, Huib D Mansvelder, Staci A Sorensen, Gabor Tamas, Ed S. Lein, Jonathan T Ting

Human cortical interneurons have been challenging to study due to high diversity and lack of mature brain tissue platforms and genetic targeting tools. We employed rapid GABAergic neuron viral labeling plus unbiased Patch-seq sampling in brain slices

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8e3b2ea84d0322f928f32322290e8a03
https://doi.org/10.1101/2022.11.08.515739

Scaled, high fidelity electrophysiological, morphological, and transcriptomic cell characterization

Autor: Lisa Kim, Brian Lee, Kimberly A. Smith, Katherine Baker, Aaron Oldre, Ed S. Lein, Rachel A. Dalley, Kristen Hadley, Thomas Braun, Brian E. Kalmbach, Sara Vargas, Jonathan T. Ting, Jim Berg, Ram Rajanbabu, Tae Kyung Kim, Agata Budzillo, Tim Jarsky, Lindsay Ng, Staci A. Sorensen, Jessica Trinh, Bosiljka Tasic, Jeremy A. Miller, Rusty Mann, Gilberto J. Soler-Llavina, Hongkui Zeng, Nathan W. Gouwens, DiJon Hill, Gabe J. Murphy

Publikováno v: eLife, Vol 10 (2021)
eLife

The Patch-seq approach is a powerful variation of the patch-clamp technique that allows for the combined electrophysiological, morphological, and transcriptomic characterization of individual neurons. To generate Patch-seq datasets at scale, we ident

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df37584b811a913c8fe77e21cca3ac2c
https://doi.org/10.7554/elife.65482

Classification of electrophysiological and morphological neuron types in the mouse visual cortex

Autor: David Sandman, Brian Lee, Michael Hawrylycz, Sara Kebede, Tom Egdorf, David Reid, Rob Young, Nivretta Thatra, Stefan Mihalas, David Feng, John W. Phillips, Rebecca de Frates, DiJon Hill, Cliff Slaughterbeck, Samuel R Josephsen, Tamara Casper, Xiaoxiao Liu, Hanchuan Peng, Peter Chong, Colin Farrell, Zhi Zhou, Sheana Parry, Jed Perkins, Brian Long, Susan M. Sunkin, Matthew Kroll, Krissy Brouner, Melissa Gorham, Aaron Szafer, Wayne Wakeman, Hong Gu, Marissa Garwood, Daniel Park, Kristen Hadley, Michael S. Fisher, Lydia Potekhina, Ed Lein, Alice Mukora, Hongkui Zeng, Nick Dee, Aaron Oldre, Lindsay Ng, Thomas Braun, Grace Williams, Tracy Lemon, Julie A. Harris, Medea McGraw, Nadezhda Dotson, Philip R. Nicovich, Amanda Gary, Rusty Mann, Alex M. Henry, Caroline Habel, Samuel Dingman, Katherine E. Link, Nathalie Gaudreault, Gilberto J. Soler-Llavina, Thuc Nghi Nguyen, Nicole Blesie, Bosiljka Tasic, Lydia Ng, Christine Cuhaciyan, Tim Jarsky, Keith B. Godfrey, Costas A. Anastassiou, Kirsten Crichton, Josef Sulc, Martin Schroedter, Dan Castelli, Miranda Robertson, Amy Bernard, Lisa Kim, Songlin Ding, Alyse Doperalski, Nathan W. Gouwens, Herman Tung, Tsega Desta, Corinne Teeter, James Harrington, Jonathan T. Ting, Kris Bickley, Anton Arkhipov, Kiet Ngo, Changkyu Lee, Jim Berg, Agata Budzillo, Emma Garren, Tanya L. Daigle, Christof Koch, Rachel A. Dalley, Eliza Barkan, Staci A. Sorensen, Gabe J. Murphy, Shiella Caldejon, Naz Taskin

Publikováno v: Nat Neurosci

Understanding the diversity of cell types in the brain has been an enduring challenge and requires detailed characterization of individual neurons in multiple dimensions. To systematically profile morpho-electric properties of mammalian neurons, we e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e51c6a86c36dcef44deb5b36ad8fc78
https://doi.org/10.1038/s41593-019-0417-0

Human cortical expansion involves diversification and specialization of supragranular intratelencephalic-projecting neurons

Autor: Sergey L. Gratiy, Sara Kebede, Chris Hill, Clare Gamlin, Jeffrey G. Ojemann, Tom Egdorf, Ed S. Lein, Lydia Potekhina, Alice Mukora, Shea Ransford, Matthew Mallory, Tim S. Heistek, Jonathan T. Ting, Gábor Tamás, Philip C. De Witt Hamer, Rebecca de Frates, Medea McGraw, Gábor Molnár, Jim Berg, Szabina Furdan, Patrick R. Hof, Natalia A. Goriounova, David Feng, David Reid, Elliot R. Thomsen, Michael Tieu, Katelyn Ward, C. Dirk Keene, Florence D’Orazi, Mean Hwan Kim, Daniel Park, Amy Torkelson, Agata Budzillo, Katherine Baker, Michael Hawrylycz, Krissy Brouner, Andrew L. Ko, DiJon Hill, Kyla Berry, Peter Chong, Jessica Trinh, Desiree A. Marshall, Katherine E. Link, Brian Lee, Jasmine Bomben, Aaron Szafer, Gabe J. Murphy, Viktor Szemenyei, Madie Hupp, Lauren Alfiler, Nick Dee, Zizhen Yao, Luke Esposito, Tamara Casper, Erica J. Melief, Susan M. Sunkin, Lindsay Ng, Hongkui Zeng, Pál Barzó, Allison Beller, Lydia Ng, Charles Cobbs, Darren Bertagnolli, Kiet Ngo, Bosiljka Tasic, John W. Phillips, Christine Rimorin, Alex M. Henry, Aaron Oldre, Michelle Maxwell, Wayne Wakeman, Delissa McMillen, Amanda Gary, Tsega Desta, Nathan Hansen, Hong Gu, Julie Nyhus, Staci A. Sorensen, Gáspár Oláh, Thomas Chartrand, Kirsten Crichton, Matthew Kroll, Josef Sulc, Jeremy A. Miller, Amy Bernard, Lisa Kim, Herman Tung, Idan Segev, Kristen Hadley, David Sandman, Anoop P. Patel, Colin Farrell, Allan R. Jones, Lisa Keene, Sander Idema, Changkyu Lee, Stephanie Mok, Augustin Ruiz, Caitlin S. Latimer, Tim Jarsky, Kris Bickley, Anton Arkhipov, Ramkumar Rajanbabu, Thomas Braun, Costas A. Anastassiou, Anatoly Buchin, Nathan W. Gouwens, Philip R. Nicovich, Richard G. Ellenbogen, Olivia Fong, Grace Williams, Rachel Enstrom, Rachel A. Dalley, Daniel L. Silbergeld, Attila Ozsvár, Kimberly A. Smith, Ryder P. Gwinn, Songlin Ding, Rafael Yuste, Manuel Ferreira, Victoria Omstead, Samuel Dingman Lee, Norbert Mihut, Hanchuan Peng, Brian E. Kalmbach, Eliza Barkan, Melissa Gorham, Boaz P. Levi, Trygve E. Bakken, Jeff Goldy, Djai B. Heyer, Nadezhda Dotson, Rusty Mann, Rebecca D. Hodge, Christof Koch, René Wilbers, Leona Mezei, Eline J. Mertens, Jae-Geun Yoon, Anna A. Galakhova, Christina A. Pom, Trangthanh Pham, Alexandra Glandon, Christiaan P. J. de Kock, Lucas T. Graybuck, Huibert D. Mansvelder

The neocortex is disproportionately expanded in human compared to mouse, both in its total volume relative to subcortical structures and in the proportion occupied by supragranular layers that selectively make connections within the cortex and other

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5b6d63820fb05a354d1dab2b84fb32f

Toward an Integrated Classification of Cell Types: Morphoelectric and Transcriptomic Characterization of Individual GABAergic Cortical Neurons

Autor: Kimberly A. Smith, Matthew Kroll, Sara Kebede, Susan M. Sunkin, David Reid, Nadezhda Dotson, Rusty Mann, DiJon Hill, Kara Ronellenfitch, Shea Ransford, Hongkui Zeng, David Feng, Jasmine Bomben, Bosiljka Tasic, Rachel Enstrom, Jessica Trinh, Matthew Mallory, Aaron Szafer, Rachel A. Dalley, Aaron Oldre, Amanda Gary, Eliza Barkan, Nick Dee, Lydia Ng, Tae Kyung Kim, Ed S. Lein, Colin Farrell, Tamara Casper, Tom Egdorf, Kirsten Crichton, Josef Sulc, Fahimeh Baftizadeh, Katelyn Ward, Kirsten Hadley, Alex M. Henry, Alice Pom, Brian Lee, Uygar Sümbül, Lisa Kim, Tim Jarsky, Madie Happ, Wayne Wakeman, Lauren Ellingwood, Luke Esposito, Daniel Park, Tanya L. Daigle, Darren Bertagnolli, Lucas T. Graybuck, Olivia Fong, Philip R. Nicovich, Gabe J. Murphy, Michelle Maxwell, Lindsay Ng, Rebeeca de Frates, Rohan Gala, Alice Mukora, Delissa McMillen, Miranda Robertson, Thanh Pham, Samuel Dingman Lee, Kris Bickley, Anton Arkhipov, Osnat Penn, Staci A. Sorensen, Alexandra Glandon, Zizhen Yao, Amy Torkelson, Jonathan T. Ting, Lauren Alfiler, Ramkumar Rajanbabu, Kiet Ngo, Kirssy Brouner, David Sandman, Michael Tieu, Michael Hawrylycz, Nathan W. Gouwens, Hanchuan Peng, Zhi Zhou, Jeff Goldy, Hong Gu, Herman Tung, Medea McGraw, Lyida Potekhina, Katherine Baker, Tsega Desta, Christof Koch, Changkyu Lee, Melissa Gorham, Clare Gamlin, Augustin Ruiz, Grace Williams, Jim Berg, Kyla Berry, Katherine E. Link, Agata Budzillo, Christine Rimorin, Thomas Braun

Publikováno v: SSRN Electronic Journal.

Neurons are frequently classified into distinct groups or cell types on the basis of structural, physiological, or genetic attributes. To better constrain the definition of neuronal cell types, we characterized the transcriptomes and intrinsic physio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3e0f199bc6125bc983caae4340b6bbde
https://doi.org/10.2139/ssrn.3544405