Zobrazeno 1 - 10
of 53
pro vyhledávání: '"Dhinakar S. Kompala"'
Publikováno v:
Biotechnology Progress. 33:913-922
As microbial secretory expression systems have become well developed for microbial yeast cells, such as Saccharomyces cerevisiae and Pichia pastoris, it is advantageous to develop high cell density continuous perfusion cultures of microbial yeast cel
Autor:
William E. Bentley, Noushin Mirjalili, Dana C. Andersen, Robert H. Davis, Dhinakar S. Kompala, Introduction by William E. Bentley
Publikováno v:
Biotechnology and Bioengineering. 102:1283-1297
Autor:
Kutlu O. Ulgen, Mehmet M. Altintas, Darryl E. Palmer-Toy, Dhinakar S. Kompala, Vivian E. Shih, Jochen Reiser
Publikováno v:
Current Chemical Biology. 2:68-82
The central metabolism of a cell can determine its short- and long-term structure and function. When a disease state arises, the metabolism (i.e., the transportation of nutrients into the cells, the overall substrate utilization and produc- tion, syn
Publikováno v:
Biotechnology and Bioengineering. 94:273-295
Zymomonas mobilis engineered to express four heterologous enzymes required for xylose utilization ferments xylose along with glucose. A network of pentose phosphate (PP) pathway enzymatic reactions interacting with the native glycolytic Entner Doudor
Publikováno v:
Biotechnology Progress. 20:1402-1407
The primary advantage of an inducible promoter expression system is that production of the recombinant protein can be biochemically controlled, allowing for the separation of unique growth and production phases of the culture. During the growth phase
Autor:
Matthew L. Lipscomb, Dhinakar S. Kompala, Mark C. Mowry, Rohaizah I. James, Venkata R. M. Mangalampalli, Alyssa K. Johnson
Publikováno v:
Cytotechnology. 38:23-35
Expression levels of reporter protein driven by Mouse Mammary Tumor Virus Promoter system were improved by expressing its specific transcription factor (glucocorticoid receptor) from a different expression vector. The vector that expresses glucocorti
Publikováno v:
Applied Biochemistry and Biotechnology. :341-356
Zymomonas mobilis has been metabolically engineered to broaden its substrate utilization range to include D-xylose and L-arabinose. Both genomically integrated and plasmid-bearing Z. mobilis strains that are capable of fermenting the pentose D-xylose
Publikováno v:
Biotechnology and Bioengineering. 50:514-520
Large-scale cultivation of murine bone marrow cells was accomplished in an airlift packed bed bioreactor system designed to mimic the in vivo bone marrow environment. The attachment-dependent stromal cell population, which provides the necessary micr
Publikováno v:
Biotechnology and Bioengineering. 67:134-140
The mouse mammary tumor virus (MMTV) promoter is induced by the addition of a glucocorticoid hormone or analog such as dexamethasone. The hormone binds to its specific transcription factor, glucocorticoid receptor (GR), and the activated complex then
Publikováno v:
Cytotechnology. 28:73-80
Protein synthesis in mammalian cells can be observed in two strikingly different patterns: 1) production of monoclonal antibodies in hybridoma cultures is typically inverse growth associated and 2) production of most therapeutic glycoproteins in reco