Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Dharani D. Dubey"'
Publikováno v:
microPublication Biology
DNA replication in eukaryotes is initiated by the orchestrated assembly and association of initiator proteins (heterohexameric Origin Recognition Complex, ORC) on the replication origins. These functionally conserved proteins play significant roles i
Autor:
Vishnu P. Tripathi, Dharani D. Dubey
Publikováno v:
Chromosoma. 126(4)
Eukaryotic replication origins are highly variable in their activity and replication timing. The nature and role of cis-acting regulatory sequences that control chromosomal replication timing is not well defined. In the fission yeast, Schizosaccharom
Publikováno v:
Genes to Cells. 15:1-12
Using a two-dimensional gel electrophoresis origin mapping technique and cell synchronization, we have studied replication timing and mapped origins in a 75-kb region of chromosome 2 of Schizosaccharomyces pombe. Three of the five mapped origins are
Publikováno v:
Genes & Development. 17:330-335
Euchromatin, which has an open structure and is frequently transcribed, tends to replicate in early S phase. Heterochromatin, which is more condensed and rarely transcribed, usually replicates in late S phase. Here, we report significant deviation fr
Publikováno v:
PLoS Genetics, Vol 10, Iss 4, p e1004344 (2014)
PLoS Genetics
PLoS Genetics
Specification of the centromere location in most eukaryotes is not solely dependent on the DNA sequence. However, the non-genetic determinants of centromere identity are not clearly defined. While multiple mechanisms, individually or in concert, may
Publikováno v:
Chromosoma. 108:471-484
According to the current paradigm, replication foci are discrete sites in the interphase nucleus where assemblies of DNA replication enzymes simultaneously elongate the replication forks of 10-100 adjacent replicons (each approximately 100 kbp). Here
Publikováno v:
Current Biology. 6:467-473
Background: In the budding yeast, Saccharomyces cerevisiae, each DNA replication origin is associated with an autonomously replicating sequence (ARS) element. Each element contains several modules, including an essential close match to the 11 base-pa
Publikováno v:
Gene. 164:173-177
We have constructed a new multipurpose stable shuttle vector for the fission yeast Schizosaccharomyces pombe (Sp). Plasmid pDblet was designed to provide convenient features for molecular work and to overcome the inconveniences of previously designed
Publikováno v:
Chromosoma. 103:414-422
We have previously reported that the replication origin region located near the ura4 gene on chromosome III of the fission yeast, Schizosaccharomyces pombe, contains three closely spaced origins, each associated with an autonomously replicating seque
Autor:
Leslie R. Davis, James R. Broach, Scott A. Greenfeder, Jiguang Zhu, Loke Y. Ong, Carol S. Newlon, Joel A. Huberman, Dharani D. Dubey
Publikováno v:
Molecular and Cellular Biology. 11:5346-5355
The silent mating-type loci of Saccharomyces cerevisiae, HML and HMR, are flanked by transcriptional silencers that have ARS activity (i.e., they function as replication origins when in plasmids). To test whether these ARS elements are chromosomal or