X-Ray Crystal Structure and Properties of Phanta, a Weakly Fluorescent Photochromic GFP-Like Protein.

Autor: Craig Don Paul, Daouda A K Traore, Seth Olsen, Rodney J Devenish, Devin W Close, Toby D M Bell, Andrew Bradbury, Matthew C J Wilce, Mark Prescott

Publikováno v: PLoS ONE, Vol 10, Iss 4, p e0123338 (2015)

Phanta is a reversibly photoswitching chromoprotein (ΦF, 0.003), useful for pcFRET, that was isolated from a mutagenesis screen of the bright green fluorescent eCGP123 (ΦF, 0.8). We have investigated the contribution of substitutions at positions H

Externí odkaz: https://doaj.org/article/f9860e38d53a43ae9558ab56ffadc089

Evaluating the analytical validity of circulating tumor DNA sequencing assays for precision oncology

Autor: Agda Karina Eterovic, Rebecca Ringler, Binsheng Gong, Jiashi Wang, Christopher E. Mason, Garima Kushwaha, Abhisek Ghosal, James C. Willey, Zhihong Zhang, Wenzhong Xiao, Natalia Novoradovskaya, Joshua Xu, Mary Yang, Zhiguang Li, Leihong Wu, Philippe Szankasi, Paul Rindler, Suman Verma, Simon Cawley, Daniel Stetson, Yilin Zhang, Fergal Casey, Nathan Haseley, Fujun Qiu, Paula Proszek, Mirna Jarosz, Ira W. Deveson, Kevin Lai, Donald J. Johann, Rebecca Kusko, Haowen Tan, Tim R. Mercer, Chia Jung Chang, Kristina Giorda, Tieliu Shi, Blake Burgher, Kyle Horvath, Leming Shi, Pierre R. Bushel, Guangchun Chen, Melissa Smith, Igor Stevanovski, Devin W. Close, Liang Feng, Lin-ya Tang, Liang-Chun Liu, Todd Richmond, Wendell D. Jones, Daniel Duncan, Andreas Scherer, Jorge Dinis, Chang Xu, Rita Shaknovich, Tom Morrison, Ping Song, Nikola Tom, Weida Tong, Quan Zhen Li, Lihyun Sun, Charles Ma, Dalila B. Megherbi, Robert Sebra, Sean Glenn, Narasimha Marella, Ambica Bhandari, Jennifer S. LoCoco, Zhichao Liu, Jeoffrey Schageman, Mehdi Pirooznia, Scott Happe, Hanane Arib, Maya Strahl, Dan Li, Hunter Best, Venkat J. Thodima, Amelia Raymond, Jeffrey Conroy, Mike Hubank, Piotr A. Mieczkowski, Carlos Pabón-Peña, James Blackburn, Li-Yuan Hung, Sa Zhang, Bindu Swapna Madala, Guangliang Zhang, Jonathan Choi

Publikováno v: Nature biotechnology

Circulating tumor DNA (ctDNA) sequencing is being rapidly adopted in precision oncology, but the accuracy, sensitivity, and reproducibility of ctDNA assays is poorly understood. Here we report the findings of a multi-site, cross-platform evaluation o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f3d76ae77be6b6d242d19ba6f29eaf8
http://europepmc.org/articles/PMC8434938

HLA genotyping in the clinical laboratory: comparison of next-generation sequencing methods

Autor: Devin W. Close, Eszter Lazar-Molnar, Julio C. Delgado, Attila Kumánovics, Tracie Profaizer

Publikováno v: HLA. 88:14-24

Implementation of human leukocyte antigen (HLA) genotyping by next-generation sequencing (NGS) in the clinical lab brings new challenges to the laboratories performing this testing. With the advent of commercially available HLA-NGS typing kits, labs

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a40cacad09b98a704c26056acbba2c19
https://doi.org/10.1111/tan.12850

Thermal green protein, an extremely stable, nonaggregating fluorescent protein created by structure-guided surface engineering

Autor: Matthew C.J. Wilce, Reginaldo C. Rocha, Andrew Bradbury, Riley Payne, Daouda A K Traore, Joseph Rucker, Geoffrey S. Waldo, Craig B Don Paul, Patricia S. Langan, Mark Prescott, Randal Halfmann, Devin W. Close

Publikováno v: Proteins: Structure, Function, and Bioinformatics. 83:1225-1237

In this article, we describe the engineering and X-ray crystal structure of Thermal Green Protein (TGP), an extremely stable, highly soluble, non-aggregating green fluorescent protein. TGP is a soluble variant of the fluorescent protein eCGP123, whic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::aeb8e6c77b94dffef3868edf79addcac
https://doi.org/10.1002/prot.24699

Corrigendum to 'Evolution and Characterization of a New Reversibly Photoswitching Chromogenic Protein, Dathail' [J. Mol. Biol., 428, (2016), 1776-89]

Autor: Andrey Kovalevsky, Devin W. Close, Koushik Ghosh, Patricia S. Langan, James P. Freyer, Andrew Bradbury, Csaba Kiss, Leighton Coates, Reginaldo C. Rocha, Geoff S. Waldo

Publikováno v: Journal of molecular biology. 428(20)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e5a03f8915d777bdafc9e0196a29e01
https://pubmed.ncbi.nlm.nih.gov/27000644