Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Devin L Trudeau"'
Exploring the Mechanism Responsible for Cellulase Thermostability by Structure-Guided Recombination.
Autor:
Chia-Jung Chang, Cheng-Chung Lee, Yueh-Te Chan, Devin L Trudeau, Mei-Huey Wu, Chih-Hsuan Tsai, Su-May Yu, Tuan-Hua David Ho, Andrew H-J Wang, Chwan-Deng Hsiao, Frances H Arnold, Yu-Chan Chao
Publikováno v:
PLoS ONE, Vol 11, Iss 3, p e0147485 (2016)
Cellulases from Bacillus and Geobacillus bacteria are potentially useful in the biofuel and animal feed industries. One of the unique characteristics of these enzymes is that they are usually quite thermostable. We previously identified a cellulase,
Externí odkaz:
https://doaj.org/article/f853fd8d2b9244558a83936be979dec6
Autor:
Devin L. Trudeau, Dan S. Tawfik
Publikováno v:
Current Opinion in Biotechnology. 60:46-52
The advent of laboratory directed evolution yielded a fruitful crosstalk between the disciplines of molecular evolution and bio-engineering. Here, we outline recent developments in both disciplines with respect to how one can identify the best starti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7be0c14518582b919ccaa5aa30ed582
https://doi.org/10.1016/j.copbio.2018.12.002
https://doi.org/10.1016/j.copbio.2018.12.002
Publikováno v:
Metabolic Engineering
To meet the increasing global demand of biodiesel over the next decades, alternative methods for producing one of the key constituents of biodiesel (e.g. fatty acid methyl esters (FAMEs)) are needed. Algal biodiesel has been a long-term target compro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a56ca91e871f785b3a9ff0197679312
https://pubmed.ncbi.nlm.nih.gov/31821864
https://pubmed.ncbi.nlm.nih.gov/31821864
Publikováno v:
Molecular Biology and Evolution. 33:2633-2641
Ancestral reconstruction provides instrumental insights regarding the biochemical and biophysical characteristics of past proteins. A striking observation relates to the remarkably high thermostability of reconstructed ancestors. The latter has been
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0def1289dfb086f374cfe6e25f656a20
https://doi.org/10.1093/molbev/msw138
https://doi.org/10.1093/molbev/msw138
Publikováno v:
Biophysical Journal. 118:45a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::55bdedf79bc2bc5366b6e99c5ab9e444
https://doi.org/10.1016/j.bpj.2019.11.425
https://doi.org/10.1016/j.bpj.2019.11.425
Autor:
Marieke Scheffen, Arren Bar-Even, Christian Edlich-Muth, Moshe Goldsmith, Devin L. Trudeau, Olga Khersonsky, Charles A. R. Cotton, Tobias J. Erb, Dan S. Tawfik, Jan Zarzycki, Sarel J. Fleishman, Ziv Avizemer
Publikováno v:
Proceedings of the National Academy of Sciences
'Proceedings of the National Academy of Sciences of the USA ', vol: 115, pages: E11455-E11464 (2018)
Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America 115(49), E11455 – E11464 (2018). doi:10.1073/pnas.1812605115
'Proceedings of the National Academy of Sciences of the USA ', vol: 115, pages: E11455-E11464 (2018)
Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America 115(49), E11455 – E11464 (2018). doi:10.1073/pnas.1812605115
Significance Photorespiration limits plant carbon fixation by releasing CO2 and using cellular resources to recycle the product of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) oxygenation, 2-phosphoglycolate. We systematically designed s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f3281a500596b2ac2aa0573d01fbad4
https://doi.org/10.1073/pnas.1812605115
https://doi.org/10.1073/pnas.1812605115
Autor:
Haim Leader, Sarel J. Fleishman, Shelly Rogotner, Rosalie Lipsh, Dan S. Tawfik, Moshe Goldsmith, Pep Amengual-Rigo, Devin L. Trudeau, Olga Khersonsky, Victor Guallar, Ziv Avizemer, Jaime Prilusky, Yacov Ashani, Orly Dym
Publikováno v:
Molecular cell. 72(1)
Substantial improvements in enzyme activity demand multiple mutations at spatially proximal positions in the active site. Such mutations, however, often exhibit unpredictable epistatic (non-additive) effects on activity. Here we describe FuncLib, an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73cd7857a1957c25b1a4abbd9b86728d
https://pubmed.ncbi.nlm.nih.gov/30270109
https://pubmed.ncbi.nlm.nih.gov/30270109
Autor:
Gili Ben-Nissan, Mark Tarnavsky, Lianet Noda Garcia, Dan S. Tawfik, Devin L. Trudeau, Michal Sharon, Galina Arkind, Jinrui Gan
Publikováno v:
Analytical Chemistry
Determining the properties of proteins prior to purification saves time and labor. Here, we demonstrate a native mass spectrometry approach for rapid characterization of overexpressed proteins directly in crude cell lysates. The method provides immed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ddd8ad52f33da7137d839eb632b86b6c
https://pubmed.ncbi.nlm.nih.gov/28345863
https://pubmed.ncbi.nlm.nih.gov/28345863
Publikováno v:
Biotechnology and Bioengineering. 111:2390-2397
A major obstacle to using widely available and low-cost lignocellulosic feedstocks to produce renewable fuels and chemicals is the high cost and low efficiency of the enzyme mixtures used to hydrolyze cellulose to fermentable sugars. One possible sol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3c0e37c5e6ede65dc1dee5c2e7283d51
https://doi.org/10.1002/bit.25308
https://doi.org/10.1002/bit.25308
Publikováno v:
Current Opinion in Chemical Biology. 17:902-909
Swapping fragments among protein homologs can produce chimeric proteins with a wide range of properties, including properties not exhibited by the parents. Computational methods that use information from structures and sequence alignments have been u
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a5e09060e76b3bbce0403cee142cfe2a
https://doi.org/10.1016/j.cbpa.2013.10.007
https://doi.org/10.1016/j.cbpa.2013.10.007