Identification of DNA binding motifs of the Mycobacterium tuberculosis PhoP/PhoR two-component signal transduction system.

Autor: Mena Cimino, Christophe Thomas, Amine Namouchi, Sarah Dubrac, Brigitte Gicquel, Deshmukh N Gopaul

Publikováno v: PLoS ONE, Vol 7, Iss 8, p e42876 (2012)

BACKGROUND: The Mycobacterium tuberculosis PhoP/PhoR two-component signal transduction system controls the expression of about 2% of the genome and plays a major role in pathogenicity. However, its regulon has not been well characterized. METHODOLOGY

Externí odkaz: https://doaj.org/article/ee6047195d9a44e28905324457ed7d39

Thio- and Seleno-Dioxaphosphorinane-Constrained Dinucleotides (D-CNA): Synthesis and Conformational Study

Autor: Corinne Payrastre, Jean-Marc Escudier, Deshmukh N. Gopaul, Claudia Addamiano, Brice-Loïc Renard, Béatrice Gerland

Publikováno v: European Journal of Organic Chemistry. 2017:1450-1464

Thio- and seleno-α,β- and α,β,γ-CNA (Constrained Nucleic Acid) dinucleotides in which two or three torsional angles of the sugar/phosphate backbone are controlled within a dioxaphosphorinane structure have been prepared. Their structural determi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::202fa629e9b8a5a08643b7fa3d9a5aca
https://doi.org/10.1002/ejoc.201601568

Inosine−Uridine Nucleoside Hydrolase from Crithidia fasciculata. Genetic Characterization, Crystallization, and Identification of Histidine 241 as a Catalytic Site Residue

Autor: Massimo Degano, Vern L. Schramm, Sheryl L. Meyer, James C. Sacchettini, Deshmukh N. Gopaul

Publikováno v: Biochemistry. 35:5963-5970

Protozoa depend on purine salvage for nucleic acid synthesis. An abundant salvage enzyme in Crithidia fasciculata is the inosine-uridine nucleoside hydrolase (IU-nucleoside hydrolase). The enzyme was cloned by polymerase chain reaction techniques usi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::92ae94593892b4c2267161f838a0bad9
https://doi.org/10.1021/bi952998u

Identification of key structural determinants of the IntI1 integron integrase that influence attC x attI1 recombination efficiency

Autor: Didier Mazel, Deshmukh N. Gopaul, Gaëlle Demarre, Clara Frumerie

Publikováno v: Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, 2007, epub ahead of print. ⟨10.1093/nar/gkm709⟩
Nucleic Acids Research, 2007, epub ahead of print. ⟨10.1093/nar/gkm709⟩

The integron platform codes for an integrase (IntI) from the tyrosine family of recombinases that mediates recombination between a proximal double-strand recombination site, attI and a single-strand target recombination site, attC. The attI site is o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::486f3e30fcbe607db11e717efa45fd49
https://pubmed.ncbi.nlm.nih.gov/17884913

Three-dimensional structure of the inosine-uridine nucleoside N-ribohydrolase from Crithidia fasciculata

Autor: James C. Sacchettini, Vern L. Schramm, Giovanna Scapin, Deshmukh N. Gopaul, Massimo Degano

Publikováno v: Biochemistry. 35(19)

Protozoan parasites rely on the host for purines since they lack a de novo synthetic pathway. Crithidia fasciculata salvages exogenous inosine primarily through hydrolysis of the N-ribosidic bond using several nucleoside hydrolases. The most abundant

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a82e8faef5bdb5c20668813efe294591
https://pubmed.ncbi.nlm.nih.gov/8634238