Photocobilins integrate B12 and bilin photochemistry for enzyme control

Autor: Shaowei Zhang, Laura N. Jeffreys, Harshwardhan Poddar, Yuqi Yu, Chuanyang Liu, Kaylee Patel, Linus O. Johannissen, Lingyun Zhu, Matthew J. Cliff, Cunyu Yan, Giorgio Schirò, Martin Weik, Michiyo Sakuma, Colin W. Levy, David Leys, Derren J. Heyes, Nigel S. Scrutton

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-16 (2024)

Abstract Photoreceptor proteins utilise chromophores to sense light and trigger a biological response. The discovery that adenosylcobalamin (or coenzyme B12) can act as a light-sensing chromophore heralded a new field of B12-photobiology. Although mi

Externí odkaz: https://doaj.org/article/a0f44c530eda465fa74001ba20e7cc49

Human cryptochrome-1 confers light independent biological activity in transgenic Drosophila correlated with flavin radical stability.

Autor: Jacqueline Vieira, Alex R Jones, Antoine Danon, Michiyo Sakuma, Nathalie Hoang, David Robles, Shirley Tait, Derren J Heyes, Marie Picot, Taishi Yoshii, Charlotte Helfrich-Förster, Guillaume Soubigou, Jean-Yves Coppee, André Klarsfeld, Francois Rouyer, Nigel S Scrutton, Margaret Ahmad

Publikováno v: PLoS ONE, Vol 7, Iss 3, p e31867 (2012)

Cryptochromes are conserved flavoprotein receptors found throughout the biological kingdom with diversified roles in plant development and entrainment of the circadian clock in animals. Light perception is proposed to occur through flavin radical for

Externí odkaz: https://doaj.org/article/8d5d8baa462448fba9b217d644b2480c

Redox driven B12-ligand switch drives CarH photoresponse

Autor: Harshwardhan Poddar, Ronald Rios-Santacruz, Derren J. Heyes, Muralidharan Shanmugam, Adam Brookfield, Linus O. Johannissen, Colin W. Levy, Laura N. Jeffreys, Shaowei Zhang, Michiyo Sakuma, Jacques-Philippe Colletier, Sam Hay, Giorgio Schirò, Martin Weik, Nigel S. Scrutton, David Leys

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023)

Abstract CarH is a coenzyme B12-dependent photoreceptor involved in regulating carotenoid biosynthesis. How light-triggered cleavage of the B12 Co-C bond culminates in CarH tetramer dissociation to initiate transcription remains unclear. Here, a seri

Externí odkaz: https://doaj.org/article/001606f7b0874be9a4255441eb03bd98

A Noncanonical Tryptophan Analogue Reveals an Active Site Hydrogen Bond Controlling Ferryl Reactivity in a Heme Peroxidase

Autor: Mary Ortmayer, Florence J. Hardy, Matthew G. Quesne, Karl Fisher, Colin Levy, Derren J. Heyes, C. Richard A. Catlow, Sam P. de Visser, Stephen E. J. Rigby, Sam Hay, Anthony P. Green

Publikováno v: JACS Au, Vol 1, Iss 7, Pp 913-918 (2021)

Externí odkaz: https://doaj.org/article/7b1ea1dfa43042aa99a9e11ae0aa8261

Solution-State Inter-Copper Distribution of Redox Partner-Linked Copper Nitrite Reductases: A Pulsed Electron–Electron Double Resonance Spectroscopy Study

Autor: Tobias M. Hedison, Andreea I. Iorgu, Donato Calabrese, Derren J. Heyes, Muralidharan Shanmugam, Nigel S. Scrutton

Publikováno v: Hedison, T M, Iorgu, A I, Calabrese, D, Heyes, D J, Shanmugam, M & Scrutton, N S 2022, ' Solution-State Inter-Copper Distribution of Redox Partner-Linked Copper Nitrite Reductases: A Pulsed Electron–Electron Double Resonance Spectroscopy Study ', The journal of physical chemistry letters, pp. 6927-6934 . https://doi.org/10.1021/acs.jpclett.2c01584

Copper nitrite reductases (CuNiRs) catalyze the reduction of nitrite to form nitric oxide. In recent years, new classes of redox partner linked CuNiRs have been isolated and characterized by crystallographic techniques. Solution-state biophysical stu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ce2ea17249a1a5481531892ed3eb160
https://research.manchester.ac.uk/en/publications/b1c27d29-3fe3-4ca5-9681-635a4ef7147d