Výsledky vyhledávání - "Derme humana artificial"

ABSTRACT Sporothrix schenckii is a dimorphic fungus and the etiologic agent of human and animal sporotrichosis. This particular mycosis is endemic in South America and affects cutaneous and subcutaneous tissues. There are several clinical manifestations related according to both, the immune state of the host and intrinsic virulence factors of the pathogen. By 2007, molecular studies demonstrated that this specie comprehends a complex of cryptic species. Currently, in Brazil, the two most frequent species of the Sporothrix spp. complex found in the clinical isolates from patients with sporotrichosis are S. schenckii and S. brasiliensis. In Rio de Janeiro State, the most prevalent species is S. brasiliensis, and up to 2012, around 3000 cases in human and 4000 in cats were registered. The transmission occurs by traumatic implantation of the pathogen, which seem to use a paracellular route to disseminate, by adhering to the native subendothelial matrix (basal lamina). However, the molecules involved in the colonization and invasion processes are still unkown for S. schenckii and S. brasiliensis. The main aim of this work was to analyze the virulence profile of several strains of both species using three distinct models. The first one, an in vivo model of subcutaneous sporotrichosis, was used to analyze the extend of lesions and the dissemination pattern of the strains. The other two in vitro models, were used to analyze the adherence of the strains to the models: native subendothelial matrix (basal lamina) and artificial human dermis. To accomplish these experiments, eight strains were selected: two of S. schenckii and six of S. brasiliensis. The results showed that in the subcutaneous murine sporotrichosis model, the S. brasiliensis strains are overall more virulent when compared to S. schenckii. In contrast, the S. schenckii strains presented higher adherence to the extracellular matrix and to the artificial human dermis. Interestingly, the highest and the lowest virulent strains tested in this study belong to the S. brasiliensis species. Because of the great variability within the isolates of this species more studies are needed in order to correlate the virulence patterns and the adhesion capacity with the infective species within the Sporothrix complex. Interaction between Sporothrix schenckii complex isolates with dermal human model and native subendothelial matrix

Autor: Sanches, Glenda de Figueiredo

Publikováno v: Biblioteca Digital de Teses e Dissertações da UERJ
Universidade do Estado do Rio de Janeiro (UERJ)
instacron:UERJ

Submitted by Boris Flegr (boris@uerj.br) on 2021-01-06T20:58:38Z No. of bitstreams: 1 Glenda de Figueiredo Sanches Dissertacao completa.pdf: 3246822 bytes, checksum: 6bdd3d944373f825796ad9112cc2ae90 (MD5) Made available in DSpace on 2021-01-06T20:58:

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______3056::8f03a907231b1f4dae2600f7cca4dd23
http://www.bdtd.uerj.br/handle/1/12787

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání