Zobrazeno 1 - 10
of 38
pro vyhledávání: '"Derek K. O'Flaherty"'
Autor:
Nadia M. Schoonhoven, Derek K. O’Flaherty, Francis P. McManus, Lauralicia Sacre, Anne M. Noronha, M. Judith Kornblatt, Christopher J. Wilds
Publikováno v:
Molecules, Vol 22, Iss 11, p 1948 (2017)
O6-Alkylguanine-DNA alkyltransferases (AGTs) are proteins responsible for the removal of mutagenic alkyl adducts at the O6-atom of guanine and O4-atom of thymine. In the current study we set out to understand the role of the Ser134 residue in the Esc
Externí odkaz:
https://doaj.org/article/57129a90d2c542cfa3f6394bf5b13f07
Publikováno v:
Journal of the American Chemical Society. 143:3267-3279
Recent advances in prebiotic chemistry are beginning to outline plausible pathways for the synthesis of the canonical ribonucleotides and their assembly into oligoribonucleotides. However, these reaction pathways suggest that many noncanonical nucleo
Autor:
Mark K Schlegel, Derek K O'Flaherty, Maja M. Janas, Jason A. Gilbert, Namrata D. Erande, Ivan Zlatev, Shigeo Matsuda, Yongfeng Jiang, June Qin, Pawan Kumar, Rohan Degaonkar, Martha Mendez, Masaaki Akabane-Nakata, Muthiah Manoharan, Martin Egli, Lauren Blair Woods
Publikováno v:
Nucleic Acids Research
We recently reported the synthesis of 2′-fluorinated Northern-methanocarbacyclic (2′-F-NMC) nucleotides, which are based on a bicyclo[3.1.0]hexane scaffold. Here, we analyzed RNAi-mediated gene silencing activity in cell culture and demonstrated
Publikováno v:
Angewandte Chemie (International Ed. in English)
The non‐enzymatic replication of the primordial genetic material is thought to have enabled the evolution of early forms of RNA‐based life. However, the replication of oligonucleotides long enough to encode catalytic functions is problematic due
Autor:
Jack W. Szostak, Tom H. Wright, Aleksandar Radakovic, Wen Zhang, Constantin Giurgiu, Lijun Zhou, Derek K. O'Flaherty
Publikováno v:
Journal of the American Chemical Society
Achieving multiple cycles of RNA replication within a model protocell would be a critical step toward demonstrating a path from prebiotic chemistry to cellular biology. Any model for early life based on an "RNA world" must account for RNA strand clea
Publikováno v:
Journal of the American Chemical Society
Efficiently copying mixed-sequence oligonucleotide templates nonenzymatically is a long-standing problem both with respect to the origin of life, and with regard to bottom up efforts to synthesize artificial living systems. Here we report an efficien
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America
Significance The RNA world hypothesis assumes the abiotic synthesis of nucleotides, as well as their participation in nonenzymatic RNA replication. Whereas prebiotic syntheses of the canonical purine nucleotides remain inefficient, a prebiotically pl
Publikováno v:
Journal of the American Chemical Society. 142(37)
Our current understanding of the chemistry of the primordial genetic material is fragmentary at best. The chemical replication of oligonucleotides long enough to perform catalytic functions is particularly problematic because of the low efficiency of
Autor:
Jack W. Szostak, Wen Zhang, Lijun Zhou, Seohyun Chris Kim, Valeria Rondo-Brovetto, Derek K. O'Flaherty
Publikováno v:
Journal of the American Chemical Society
The abiotic synthesis of ribonucleotides is thought to have been an essential step toward the emergence of the RNA world. However, it is likely that the prebiotic synthesis of ribonucleotides was accompanied by the simultaneous synthesis of arabinonu
Publikováno v:
Bio Protoc
The RNA world hypothesis describes a scenario where early life forms relied on RNA to govern both inheritance and catalyze useful chemical reactions. Prior to the emergence of enzymes capable of replicating the RNA genome, a nonenzymatic replication