Zobrazeno 1 - 3
of 3
pro vyhledávání: '"Derek Cerchione"'
Autor:
Colin McGaw, Anthony J. Garrity, Gabrielle Z. Munoz, Jeffrey R. Haswell, Sejuti Sengupta, Elise Keston-Smith, Pratyusha Hunnewell, Alexa Ornstein, Mishti Bose, Quinton Wessells, Noah Jakimo, Paul Yan, Huaibin Zhang, Lauren E. Alfonse, Roy Ziblat, Jason M. Carte, Wei-Cheng Lu, Derek Cerchione, Brendan Hilbert, Shanmugapriya Sothiselvam, Winston X. Yan, David R. Cheng, David A. Scott, Tia DiTommaso, Shaorong Chong
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-11 (2022)
Cas12i is a genome editing platform with compact size that fits in AAV vector with short 43-mer gRNA, absence of tracrRNA, ability to process pre-crRNA, and high specificity. Here the authors present an unbiased mutational scanning approach to engine
Externí odkaz:
https://doaj.org/article/b34236ad23cc4e1cb4a7c8a576cc399b
Autor:
Derek Cerchione, Katherine Loveluck, Eric L Tillotson, Fred Harbinski, Jen DaSilva, Chase P Kelley, Elise Keston-Smith, Cecilia A Fernandez, Vic E Myer, Hariharan Jayaram, Barrett E Steinberg
Publikováno v:
PLoS ONE, Vol 15, Iss 4, p e0231716 (2020)
RNA-guided endonucleases such as Cas9 provide efficient on-target genome editing in cells but may also cleave at off-target loci throughout the genome. Engineered variants of Streptococcus pyogenes Cas9 (SpCas9) have been developed to globally reduce
Externí odkaz:
https://doaj.org/article/2e8e195ece5248d19bf5391aebfa0fa0
SMOOT libraries and phage-induced directed evolution of Cas9 to engineer reduced off-target activity
Autor:
Hariharan Jayaram, Derek Cerchione, Barrett Ethan Steinberg, Jen DaSilva, Elise Keston-Smith, Chase P. Kelley, Eric L. Tillotson, Fred Harbinski, Cecilia Fernández, Katherine Loveluck, Vic E. Myer
Publikováno v:
PLoS ONE
PLoS ONE, Vol 15, Iss 4, p e0231716 (2020)
PLoS ONE, Vol 15, Iss 4, p e0231716 (2020)
RNA-guided endonucleases such as Cas9 provide efficient on-target genome editing in cells but may also cleave at off-target loci throughout the genome. Engineered variants of Streptococcus pyogenes Cas9 (SpCas9) have been developed to globally reduce