Natural biocide cocktails: Combinatorial antibiotic effects of prodigiosin and biosurfactants.

Autor: Jennifer Hage-Hülsmann, Alexander Grünberger, Stephan Thies, Beatrix Santiago-Schübel, Andreas Sebastian Klein, Jörg Pietruszka, Dennis Binder, Fabienne Hilgers, Andreas Domröse, Thomas Drepper, Dietrich Kohlheyer, Karl-Erich Jaeger, Anita Loeschcke

Publikováno v: PLoS ONE, Vol 13, Iss 7, p e0200940 (2018)

Bacterial secondary metabolites are naturally produced to prevail amongst competitors in a shared habitat and thus represent a valuable source for antibiotic discovery. The transformation of newly discovered antibiotic compounds into effective drugs

Externí odkaz: https://doaj.org/article/bcd137b3eb2945cdadbc59a6436e5251

Optochemical Control of Bacterial Gene Expression: Novel Photocaged Compounds for Different Promoter Systems

Autor: Vera Ophoven, Claus Bier, Fabian Hogenkamp, Nora Lisa Bitzenhofer, Thomas Drepper, Mona Haase, Dennis Binder, Jörg Pietruszka, Karl-Erich Jaeger, Fabienne Hilgers

Publikováno v: ChemBioChem 23(1), e202100467 (2022). doi:10.1002/cbic.202100467

Photocaged compounds are applied for implementing precise, optochemical control of gene expression in bacteria. To broaden the scope of UV-light-responsive inducer molecules, six photocaged carbohydrates were synthesized and photochemically character

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8bf8e824fbea8a3334d2fedbf521b27
https://hdl.handle.net/2128/30077

Light-Controlled Cell Factories: Employing Photocaged Isopropyl-β- d -Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum

Autor: Volker F. Wendisch, Dietrich Kohlheyer, Anita Loeschcke, Julia Frunzke, Jörg Pietruszka, Claus Bier, Petra Peters-Wendisch, Thomas Drepper, Jonas Frohwitter, Regina Mahr, Dennis Binder, Alexander Grünberger, Karl-Erich Jaeger

Publikováno v: Applied and Environmental Microbiology. 82:6141-6149

Precise control of microbial gene expression resulting in a defined, fast, and homogeneous response is of utmost importance for synthetic bio(techno)logical applications. However, even broadly applied biotechnological workhorses, such as Corynebacter

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::56df4c82c3a56e2e85400da25953349e
https://doi.org/10.1128/aem.01457-16

Homogenizing bacterial cell factories: Analysis and engineering of phenotypic heterogeneity

Autor: Wolfgang Wiechert, Dietrich Kohlheyer, Frank Delvigne, Dennis Binder, Alexander Grünberger, Thomas Drepper, Karl-Erich Jaeger

Publikováno v: Metabolic engineering. 42

In natural habitats, microbes form multispecies communities that commonly face rapidly changing and highly competitive environments. Thus, phenotypic heterogeneity has evolved as an innate and important survival strategy to gain an overall fitness ad

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea5bf3d8803bc9dde715f1fe5de256d0
https://pubmed.ncbi.nlm.nih.gov/28645641

Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system

Autor: Jörg Pietruszka, Georg Wandrey, Karl-Erich Jaeger, Claus Bier, Thomas Drepper, Kyra Hoffmann, Jochen Büchs, Dennis Binder

Publikováno v: Microbial Cell Factories
Microbial cell factories 15, 63 (2016). doi:10.1186/s12934-016-0461-3
Microbial cell factories 15(63), 16 Seiten (2016). doi:10.1186/s12934-016-0461-3

Background Inducible expression systems are frequently used for the production of heterologous proteins. Achieving maximum product concentrations requires induction profiling, namely the optimization of induction time and inducer concentration. Howev

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c73919b7c2233cbfd00996f18252edbd
http://europepmc.org/articles/PMC4842301