Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Denise M. Lowe"'
Autor:
Bill Leavens, Jonathan P. Hutchinson, Carl Haslam, Damian J. Mole, John Liddle, Scott P. Webster, Beverley A. Yard, Argyrides Argyrou, Erica Christodoulou, Margaret Binnie, Paul Hissey, P. Hardwicke, Kris Wilson, Denise M. Lowe, Tony W. Dean, Iain Uings, Michelle Gee
Publikováno v:
Journal of biomolecular screening. 19(4)
Kynurenine 3-monooxygenase (KMO) is a therapeutically important target on the eukaryotic tryptophan catabolic pathway, where it converts L-kynurenine (Kyn) to 3-hydroxykynurenine (3-HK). We have cloned and expressed the human form of this membrane pr
Publikováno v:
European Journal of Biochemistry. 206:437-440
Human-immunodeficiency-virus-type-1 reverse transcriptase exists in virions as a heterodimer of a M(r) 66,000 subunit and its C-terminally truncated form of M(r) 51,000, but, when expressed as a recombinant M(r) 66,000 protein, a mixture of heterodim
Publikováno v:
FEBS Letters. 282:231-234
Two conserved sequence motifs, occurring in HIV-1 reverse transcriptase at residues 110–116 and 183–190, have been studied using site-directed mutagenesis of the cloned gene. In particular, aspariates at positions 185 and 186 have each been mutat
Autor:
O. Matsumoto, R. Griest, Brendan Larder, David Phillips, Denise M. Lowe, D. O. Somers, C. Ross, Garry L. Taylor, David K. Stammers, Margaret Tisdale, Kenneth L. Powell, Dorothy J. M. Purifoy, E Y Jones, Elspeth F. Garman, David I. Stuart, Graham Darby
Publikováno v:
JOURNAL OF CRYSTAL GROWTH. 126(2-3)
Extensive studies on the crystallization of HIV-1 reverse transcriptase (RT) have yielded several crystal forms, two of which show diffraction to minimum Bragg spacings of 6 A or better. Type 1 crystals belong to the space group P2 1 2 1 2 1 with uni
Autor:
E Y Jones, Graham Darby, Brendan Larder, Elspeth F. Garman, R. Griest, David K. Stammers, Garry L. Taylor, Kenneth L. Powell, Purifoy Djm., David I. Stuart, Denise M. Lowe, David Phillips, Margaret Tisdale
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fe737b0b595ad2dd502d20d321dff4f1
https://doi.org/10.1016/b978-0-12-438745-4.50028-8
https://doi.org/10.1016/b978-0-12-438745-4.50028-8
Autor:
Denise M. Lowe, Greg Winter, Mary M.Y. Waye, Alan R. Fersht, Hugues Bedouelle, Anthony J. Wilkinson, Paul Carter
Publikováno v:
Biochimie. 67:737-743
Resume Le gene codant pour la tyrosyl tRNA synthetase de Bacillus stearothermophilus a ete systematiquement altere en utilisant des oligonucleotides synthetiques comme mutagenes. La construction des mutations a ete facilitee par l'utilisation de souc
Autor:
Paul Carter, Jian-Ping Shi, J.W. Knill-Jones, Mary M.Y. Waye, Peter Brick, David M. Blow, Denise M. Lowe, Alan R. Fersht, Greg Winter, Anthony J. Wilkinson
Publikováno v:
Nature. 314:235-238
The role of complementary hydrogen bonding as a determinant of biological specificity has been examined by protein engineering of the tyrosyl-tRNA synthetase. Deletion of a side chain between enzyme and substrate to leave an unpaired, uncharged hydro
Autor:
Denise M. Lowe, P. K. Tubbs
Publikováno v:
Analytical Biochemistry. 132:276-284
Bromo[1-14C]acetyl-CoA has been prepared from CoASH and the N-hydroxysuccinimide ester of bromo[1-14C]acetic acid, and unlabeled bromoacetyl-CoA by reaction of CoASH with bromoacetyl bromide. The products were purified by high-pressure liquid chromat
Autor:
Brendan Larder, P. Ertl, Dorothy J. M. Purifoy, David K. Stammers, S. Kemp, Graham Darby, Margaret Tisdale, Denise M. Lowe, C. Bradley, Kenneth L. Powell
Publikováno v:
The Journal of antimicrobial chemotherapy. 23(SUPPL)
The reverse transcriptase (RT) of HIV is an important target for chemotherapy as demonstrated by the effective treatment of AIDS patients with zidovudine, a potent inhibitor of RT. Structural studies of HIV RT were therefore undertaken with a view to
Publikováno v:
Biochemistry. 25(8)
We report the cloning and sequence analysis of the gene for the tyrosyl-tRNA synthetase from Bacillus caldotenax and properties of the gene product. The amino acid sequence of the tyrosyl-tRNA synthetase was found to be 99% homologous with the corres