Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Denise C. Monteleone"'
Autor:
Denise C. Monteleone, Daniel van der Lelie, David Moreels, Garry Crosson, Jeffrey P. Fitts, Craig Garafola, Safiyh Taghavi
Publikováno v:
Environmental Science and Pollution Research. 15:481-491
The subsurface at the Oak Ridge Field Research Center represents an extreme and diverse geochemical environment that places different stresses on the endogenous microbial communities, including low pH, elevated nitrate concentrations, and the occurre
Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis
Publikováno v:
ELECTROPHORESIS. 22:843-854
DNA damages that can be converted to single- or double strand breaks can be quantified by separating DNA by gel electrophoresis and obtaining a quantitative image of the resulting distribution of DNA in the gel. We review the theory of this method an
Publikováno v:
Journal of Photochemistry and Photobiology B: Biology. 40:14-22
Modern scientific instruments can produce huge quantities of data, usually in digital form. However, data acquisition is only one of three important functions. To be useful, data must also be stored and analyzed. Fortunately, the same computer-based
Publikováno v:
Analytical Biochemistry. 239:136-144
Finish-line imaging, in which DNA or other macromolecules are detected after electrophoresis for a constant distance, usually improves resolution compared to snapshot imaging, in which molecules are electrophoresed for a constant time in an apparatus
Autor:
John C. Sutherland, Zbigniew Kolber, Krzysztof Polewski, John Trunk, Denise C. Monteleone, S.L. Kramer
Publikováno v:
Review of Scientific Instruments. 65:2562-2567
The pulsed nature or ‘‘time structure’’ of synchrotron radiation from electron storage rings is used to measure the kinetics of the decay of electronically excited states and is particularly useful because the wavelength of excitation can be
Autor:
Denise C. Monteleone, John Trunk, Megumi Hada, Alan M. Gewirtz, Betsy M. Sutherland, Marisha A. Stanislaus, Nela Cintron-Torres, Jacques Laval, Paula V. Bennett, John C. Sutherland
Publikováno v:
Journal of radiation research.
Ionizing radiation induces clusters of DNA damages--oxidized bases, abasic sites and strand breaks--on opposing strands within a few helical turns. Such damages have been postulated to be difficult to repair, as are double strand breaks (one type of
Autor:
Chi-Chang Kao, Krzysztof Polewski, Steven L. Hulbert, Gary A. Nintzel, Erik D. Johnson, Om Singh, Qing-Li Dong, Dennis G. Carlson, John Trunk, John C. Sutherland, Denise C. Monteleone
Publikováno v:
SPIE Proceedings.
We have constructed an experimental station (beamline) at the National Synchrotron Light Source to measure circular dichroism (CD) using soft x-rays (250 {le} hv {le} 900 eV) from a time modulated elliptically polarizing wiggler. The polarization of
Autor:
John Trunk, Chun Zhang Chen, A. Emrick, Eldred A. Ribeiro, Haim Hacham, John C. Sutherland, Denise C. Monteleone, Betsy M. Sutherland
Publikováno v:
DNA Damage and Repair in Human Tissues ISBN: 9781461279037
The important role of DNA damage and repair in homeostasis has been clearly demonstrated by studies involving isolated DNA, procaryotic cells and eukaryotic cells growing in culture. The information obtainable from these model systems, while extremel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1a5bd4db1b8d454f14da61b183a4e849
https://doi.org/10.1007/978-1-4613-0637-5_4
https://doi.org/10.1007/978-1-4613-0637-5_4
Autor:
Betsy M. Sutherland, Denise C. Monteleone, John C. Sutherland, Steven E. Freeman, Richard B. Setlow, Anthony D. Blackett
Publikováno v:
Analytical Biochemistry. 158:119-129
We have developed an alkaline agarose gel method for quantitating single strand breaks in nanogram quantities of nonradioactive DNA. After electrophoresis together with molecular length standards, the DNA is neutralized, stained with ethidium bromide
Autor:
John C. Sutherland, Jo Ann Mugavero, Betsy M. Sutherland, Denise C. Monteleone, Bohai Lin, John Trunk
Publikováno v:
Analytical Biochemistry. 163:446-457
We have built an electronic imaging system based on a modified charge-coupled-device television camera that directly quantitates the distribution of fluorescence from electrophoretic gels, chromatograms, and other stationary sources. Exposure times c