A Functional Tricarboxylic Acid Cycle Operates during Growth of Bordetella pertussis on Amino Acid Mixtures as Sole Carbon Substrates.

Autor: Marie Izac, Dominique Garnier, Denis Speck, Nic D Lindley

Publikováno v: PLoS ONE, Vol 10, Iss 12, p e0145251 (2015)

It has been claimed that citrate synthase, aconitase and isocitrate dehydrogenase activities are non-functional in Bordetella pertussis and that this might explain why this bacterium's growth is sometimes associated with accumulation of polyhydroxybu

Externí odkaz: https://doaj.org/article/357b6544fadd4cadb423ac749a703b34

Genomics of Clostridium tetani

Autor: Holger Brüggemann, Denis Speck, Elzbieta Brzuszkiewicz, Diana Chapeton-Montes, Michel R. Popoff, Lucile Plourde

Publikováno v: Research in Microbiology
Research in Microbiology, Elsevier, 2015, 166 (4), pp.326-331. ⟨10.1016/j.resmic.2015.01.002⟩
Research in Microbiology, 2015, 166 (4), pp.326-331. ⟨10.1016/j.resmic.2015.01.002⟩
Brüggemann, H, Brzuszkiewicz, E, Chapeton-Montes, D, Plourde, L, Speck, D & Popoff, M R 2015, ' Genomics of Clostridium tetani ', Research in Microbiology . https://doi.org/10.1016/j.resmic.2015.01.002

International audience; Genomic information about Clostridium tetani, the causative agent of the tetanus disease, is scarce. The genome of strain E88, a strain used in vaccine production, was sequenced about 10 years ago. One additional genome (strai

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ffcf0e0082b84b59ab051ffa713ab741
https://hal-pasteur.archives-ouvertes.fr/pasteur-01770121

Recombinant Cholera Toxin B-Subunit in Escherichia coli: High-Level Secretion, Purification, and Characterization

Autor: Marie Paule Kieny, B. Bouchon, Denis Speck, N. Accart, D. Schubnel, Hanno Kolbe, Rainer Bischoff, P. Slos

Publikováno v: Protein Expr.Purif., 5(5), 518-526

The gene coding for cholera toxin subunit B (CT-B) was fused to a modified ompA signal sequence and subsequently cloned into a high expression vector based on the regulatory signals of the arabinose operon of Salmonella typhimurium. Upon induction of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff0899bd31859b027953eb1ff45df1be
https://doi.org/10.1006/prep.1994.1071

Bioconversion of pimelic acid into biotin by Bacillus sphaericus bioB transformants

Autor: Kouichi Kamogawa, Remi Gloeckler, Kazuko Kodama, Denis Speck, Ikuroh Ohsawa, Tsugio Kisou, Ikuhiro Yoneda, Yves Lemoine

Publikováno v: Journal of Fermentation and Bioengineering. 73:121-124

Bacillus sphaericus was treated with N-acetylmuramidase and transformed with different bioB expression vectors by protoplast transformation, of which only three could be introduced into B. sphaericus. After cultivating the transformants for 24 h at 2

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f4cbe91a94cc4f3f43c5173ff087002f
https://doi.org/10.1016/0922-338x(92)90475-a

Expression of alpha 1-proteinase inhibitor in Escherichia coli: effects of single amino acid substitutions in the active site loop on aggregate formation

Autor: Andreas Schulze, Eric Degryse, Robert Huber, Denis Speck, Rainer Bischoff

Publikováno v: Journal of biotechnology. 32(3)

Overproduction of eukaryotic proteins in microorganisms often leads to the formation of insoluble protein aggregates which accumulate as intracellular inclusion bodies. alpha 1-Proteinase inhibitor (alpha 1-PI) when produced as a cytoplasmic protein

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf628e53ef669a398a27cd28e3fb7191
https://pubmed.ncbi.nlm.nih.gov/7764716