Zobrazeno 1 - 2
of 2
pro vyhledávání: '"Denis S. Wright"'
Publikováno v:
Biotechnology Letters. 16:51-56
We report simple fermentation conditions for high-level extracellular production of α-lytic protease and its mutants by recombinantE. coli. Although transcription was controlled by thelac promoter, addition of the inducer isopropyl-s-D-thiogalactopy
Publikováno v:
Biochimica et biophysica acta. 1443(3)
Screening an expression library of Lysobacter enzymogenes DNA allowed us to clone a gene encoding a serine protease that cleaves synthetic substrates C-terminal to Arg and, to a lesser extent, Lys residues. The gene product, which shares sequence hom