Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Denis Fellmann"'
Autor:
Pick-Wei Lau, Anchi Cheng, Dmitry Lyumkis, Neil R. Voss, Bridget Carragher, Gabriel C. Lander, Clinton S. Potter, Christopher Irving, Craig Yoshioka, Scott M. Stagg, Denis Fellmann, James Pulokas, Anke M. Mulder
Publikováno v:
Journal of Structural Biology. 166:95-102
The use of cryo-EM and three-dimensional image reconstruction is becoming increasingly common. Our vision for this technique is to provide a straightforward manner in which users can proceed from raw data to a reliable 3D reconstruction through a pip
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7ff6ac563fd4a33241aaf65813110a3
https://doi.org/10.1016/j.jsb.2009.01.002
https://doi.org/10.1016/j.jsb.2009.01.002
Autor:
Denis Fellmann, Stephen E. Mick, John Damiano, Clinton S. Potter, David P. Nackashi, Bridget Carragher, Joel Quispe, Teddy G. Ajero
Publikováno v:
Microscopy and Microanalysis. 13:365-371
Two issues that often impact the cryo-electron microscopy (cryoEM) specimen preparation process are agglomeration of particles near hole edges in holey carbon films and variations in vitreous ice thickness. In many cases, the source of these issues w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c655127b0e8907e5f4199d49506fdb4a
https://doi.org/10.1017/s1431927607070791
https://doi.org/10.1017/s1431927607070791
Autor:
Craig Yoshioka, Ronald A. Milligan, Clinton S. Potter, Bridget Carragher, Denis Fellmann, James Pulokas
Publikováno v:
Journal of Structural Biology. 159:335-346
Visualization by electron microscopy has provided many insights into the composition, quaternary structure, and mechanism of macromolecular assemblies. By preserving samples in stain or vitreous ice it is possible to image them as discrete particles,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7880253abaa64d49a68b299fdd0b0275
https://doi.org/10.1016/j.jsb.2007.03.005
https://doi.org/10.1016/j.jsb.2007.03.005
Autor:
Denis Fellmann, James Pulokas, Clinton S. Potter, Gabriel C. Lander, Satya P. Mallick, Bridget Carragher, Joel Quispe, Radomir M. Avila, Scott M. Stagg, Anchi Cheng
Publikováno v:
Journal of Structural Biology. 155:470-481
One of the goals in developing our automated electron microscopy data acquisition system, Leginon, was to improve both the ease of use and the throughput of the process of acquiring low dose images of macromolecular specimens embedded in vitreous ice
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25ffc15faa46543b5439e5d988f4cd89
https://doi.org/10.1016/j.jsb.2006.04.005
https://doi.org/10.1016/j.jsb.2006.04.005
Publikováno v:
Microscopy Today. 14:24-29
The ultimate performance of high resolution electron microscopes depends on a variety of factors including the stability of the temperature surrounding the instrument. Variations in temperature can cause drift of the specimen, the microscope electron
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::215d7d6f1b594a0a5043bb91e4effcbb
https://doi.org/10.1017/s1551929500055152
https://doi.org/10.1017/s1551929500055152
Autor:
Denis Fellmann, James Pulokas, Scott M. Stagg, Christian Suloway, Bridget Carragher, Francisco Guerra, Joel Quispe, Clinton S. Potter, Anchi Cheng
Publikováno v:
Journal of Structural Biology. 151:41-60
We report here on the current state of our efforts in automated molecular microscopy. Our primary automated data acquisition software system, Leginon, has been completely redesigned over the past two years. The new distributed system has been develop
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f904b1f0490aaf2670f15a0ccb3cebf
https://doi.org/10.1016/j.jsb.2005.03.010
https://doi.org/10.1016/j.jsb.2005.03.010
Autor:
Chandrajit L. Bajaj, Pawel A. Penczek, Marshall Bern, Fred J. Sigworth, Clinton S. Potter, Felix de Haas, Niels Volkmann, Fabrice Mouche, Denis Fellmann, Satya P. Mallick, Richard Hall, Yuanxin Zhu, David J. Kriegman, Robert M. Glaeser, Steven J. Ludtke, Bridget Carragher, Alan M. Roseman
Publikováno v:
Journal of Structural Biology. 145:3-14
Manual selection of single particles in images acquired using cryo-electron microscopy (cryoEM) will become a significant bottleneck when datasets of a hundred thousand or even a million particles are required for structure determination at near atom
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f08340125bf04efc8ac0b8264aa8af4e
https://doi.org/10.1016/j.jsb.2003.09.033
https://doi.org/10.1016/j.jsb.2003.09.033
Publikováno v:
Journal of Structural Biology. 137:273-282
For the past year we have been using a relational database as part of an automated data collection system for cryoEM. The database is vital for keeping track of the very large number of images collected and analyzed by the automated system and essent
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27126f33fd34916b9dea816f52018426
https://doi.org/10.1016/s1047-8477(02)00002-3
https://doi.org/10.1016/s1047-8477(02)00002-3
Autor:
Craig Yoshioka, Dmitry Lyumkis, Pick-Wei Lau, Anke M. Mulder, Denis Fellmann, Joel Quispe, Neil R. Voss, Hanspeter Winkler, James Pulokas, Gabriel C. Lander, Anchi Cheng, Albert Leung, Erica L. Jacovetty, Clinton S. Potter, Christopher Irving, Bridget Carragher, Edward J. Brignole
Publikováno v:
Journal of structural biology. 169(3)
Structure determination of a novel macromolecular complex via single-particle electron microscopy depends upon overcoming the challenge of establishing a reliable 3-D reconstruction using only 2-D images. There are a variety of strategies that deal w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0241267ce3cff14ed653edf39678ab06
https://pubmed.ncbi.nlm.nih.gov/20018246
https://pubmed.ncbi.nlm.nih.gov/20018246
Autor:
Anchi Cheng, Joel Quispe, Albert Leung, Joseph S. Lam, Christian Suloway, Denis Fellmann, James Pulokas, Clinton S. Potter, Priyanka D. Abeyrathne, Bridget Carragher
Publikováno v:
Journal of structural biology. 160(3)
Screening trials to determine the presence of two-dimensional (2D) protein crystals suitable for three-dimensional structure determination using electron crystallography is a very labor-intensive process. Methods compatible with fully automated scree
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2759ec2d3c8e7b7e45adf2d2dd4324d0
https://pubmed.ncbi.nlm.nih.gov/17977016
https://pubmed.ncbi.nlm.nih.gov/17977016