Zobrazeno 1 - 10
of 64
pro vyhledávání: '"Denis C. Shaw"'
Publikováno v:
Journal of Molecular Recognition. 15:341-348
The VH domains of two human monoclonal antibodies, designated Mcg IgG1(lambda) and Yvo IgM(kappa), were particularly intractable to standard protein sequencing protocols. Peptides liberated from the VH domains of these proteins, using standard enzyma
Publikováno v:
Biochemical Genetics. 39:59-71
The major proteins of baboon milk were identified as β-lactoglobulin (βLG), α-lactalbumin (αLA), lysozyme, lactoferrin, casein, and albumin by immobiline isoelectric focusing, SDS-PAGE, immunoblotting of gels with rabbit antisera to human αLA, l
Autor:
Kaylene J. Simpson, Shoba Ranganathan, Kevin R. Nicholas, Denis C. Shaw, Juliet A. Fisher, P.A. Janssens
Publikováno v:
Journal of Biological Chemistry. 275:23074-23081
Secretion of whey acidic protein (WAP) in milk throughout lactation has previously been reported for a limited number of species, including the mouse, rat, rabbit, camel, and pig. We report here the isolation of WAP from the milk of a marsupial, the
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1476:265-278
In humans, the crystallin proteins of the ocular lens become yellow-coloured and fluorescent with ageing. With the development of senile nuclear cataract, the crystallins become brown and additional fluorophores are formed. The mechanism underlying c
Publikováno v:
Journal of Molecular Graphics and Modelling. 17:106-113
Whey acidic proteins (WAP) from the mouse, rat, rabbit, camel, and pig comprise two "four-disulfide core" domains. From a detailed analysis of all sequences containing this domain, we propose a new PROSITE motif ([KRHGVLN]-X-¿PF¿-X-[CF]-[PQSVLI]-X(
Publikováno v:
Journal of Mammalian Evolution. 5:95-105
The amino acid sequences of the α-lactalbumins of the echidna, Tachyglossus aculeatus, and the platypus, Ornithorhynchus anatinus, were compared with each other and with those of 13 eutherian and 3 marsupial species. Phylogenetic parsimony analyses,
Publikováno v:
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 118:403-410
alpha-Lactalbumin and lysozyme were each isolated from echidna (Tachyglossus aculeatus) milk by gel permeation and ion exchange chromatography. The alpha-lactalbumin modified the action of echidna milk galactosyltransferase to promote the synthesis o
Publikováno v:
Protein Science. 6:909-912
When electrospray ionization mass spectrometry (ESMS) was used to analyze purified bovine gamma E (gamma IVa)-crystallin, it yielded a relative molecular mass (M(r)) of 20.955 +/- 5. This mass is significantly different from that calculated from the
Publikováno v:
Electrophoresis. 18:1393-1398
Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) has become the method of choice for efficient separation of complex protein mixtures. Previously, analysis of the Spiroplasma melliferum proteome (protein complement of a genome) has been
Autor:
Margaret M. Sheil, Denis C. Shaw, Shanlin Fu, Jacqueline Boschenok, Randolph I. Mar, John A. Carver
Publikováno v:
Phytochemistry. 41:1265-1274
Three trypsin inhibitors from Sicyos australis, have been isolated, purified and sequenced. Following protein extraction with ammonium sulphate, the mixture of inhibitors was separated from other proteins by trypsin-affinity chromatography. Subsequen