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Development of novel methods for non-canonical myeloma protein analysis with an innovative adaptation of immunofixation electrophoresis, native top-down mass spectrometry, and middle-down de novo sequencing.

Autor: Deighan WI; Department of Clinical Chemistry, Altnagelvin Area Hospital, Londonderry, UK., Winton VJ; Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA., Melani RD; Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA., Anderson LC; Ion Cyclotron Resonance Program, National High Magnetic Field Laboratory, Tallahassee, FL, USA., McGee JP; Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA., Schachner LF; Department of Chemistry, Northwestern University, Evanston, IL, USA., Barnidge D; Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, MN, USA., Murray D; Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, MN, USA., Alexander HD; Northern Ireland Centre for Stratified Medicine, Biomedical Sciences Research Institute, Ulster University, Londonderry, UK., Gibson DS; Northern Ireland Centre for Stratified Medicine, Biomedical Sciences Research Institute, Ulster University, Londonderry, UK., Deery MJ; Cambridge Centre for Proteomics, University of Cambridge, Cambridge, UK., McNicholl FP; Department of Haematology, Altnagelvin Area Hospital, Londonderry, UK., McLaughlin J; Northern Ireland Centre for Stratified Medicine, Biomedical Sciences Research Institute, Ulster University, Londonderry, UK., Kelleher NL; Proteomics Center of Excellence & Departments of Chemistry and Molecular Biology, Northwestern University, Evanston, IL, USA., Thomas PM; Proteomics Center of Excellence & Departments of Chemistry and Molecular Biology, Northwestern University, Evanston, IL, USA.

Publikováno v: Clinical chemistry and laboratory medicine [Clin Chem Lab Med] 2020 Oct 20; Vol. 59 (4), pp. 653-661. Date of Electronic Publication: 2020 Oct 20 (Print Publication: 2021).