Traditional protocols and optimization methods lead to absent expression in a mycoplasma cell-free gene expression platform.

Autor: Sakai A, Deich CR; Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA., Nelissen FHT; Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands., Jonker AJ; Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands., Bittencourt DMC; Synthetic Biology & Bioenergy, J. Craig Venter Institute, La Jolla, CA, USA., Kempes CP; Santa Fe Institute, Santa Fe, NM, USA., Wise KS; Synthetic Biology & Bioenergy, J. Craig Venter Institute, La Jolla, CA, USA., Heus HA; Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands., Huck WTS; Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands., Adamala KP; Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA., Glass JI; Synthetic Biology & Bioenergy, J. Craig Venter Institute, La Jolla, CA, USA.

Publikováno v: Synthetic biology (Oxford, England) [Synth Biol (Oxf)] 2022 May 21; Vol. 7 (1), pp. ysac008. Date of Electronic Publication: 2022 May 21 (Print Publication: 2022).