Cloning and Characterization of a New Site-Specific Methyl-Directed ElmI Endonuclease Recognizing and Cleaving C5-methylated DNA Sequence 5’-G(5mC)^NG(5mC)-3’

Autor: Lomakovskaya En, Dedkov Vs, M. A. Abdurashitov, D. A. Gonchar, N.A.Mikhnenkova, Udal'yeva Sg, Chernukhin Va, Belichenko Oa, Degtyarev SKh

Publikováno v: Acta Naturae. 8:117-125

Putative open reading frames of MD-endonucleases have been identified in Enterobacteria genomes as a result of the search for amino acid sequences homologous to MD-endonuclease BisI. A highly conserved DNA primary structure of these open reading fram

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::80086f275f83496c2e8295e5e96b6a91
https://doi.org/10.32607/20758251-2016-8-1-117-125

Cloning and Characterization of a New Site-Specific Methyl-Directed ElmI Endonuclease Recognizing and Cleaving C5-methylated DNA Sequence 5'-G(5mC)^NG(5mC)-3'.

Autor: Chernukhin VA; SibEnzyme, Timakova St., 2/12, 630117, Novosibirsk, Russia., Gonchar DA; SibEnzyme, Timakova St., 2/12, 630117, Novosibirsk, Russia., Abdurashitov MA; SibEnzyme, Timakova St., 2/12, 630117, Novosibirsk, Russia., Belichenko OA; SibEnzyme, Timakova St., 2/12, 630117, Novosibirsk, Russia., Dedkov VS; SibEnzyme, Timakova St., 2/12, 630117, Novosibirsk, Russia., Mikhnenkova NA; SibEnzyme, Timakova St., 2/12, 630117, Novosibirsk, Russia., Lomakovskaya EN; SibEnzyme, Timakova St., 2/12, 630117, Novosibirsk, Russia., Udal'yeva SG; SibEnzyme, Timakova St., 2/12, 630117, Novosibirsk, Russia., Degtyarev SKh; SibEnzyme, Timakova St., 2/12, 630117, Novosibirsk, Russia.

Publikováno v: Acta naturae [Acta Naturae] 2016 Jan-Mar; Vol. 8 (1), pp. 117-25.

Substrate specificity and biochemical properties of M3.BstF5I DNA methyltransferase from the BstF5I restriction-modification system.

Autor: Chernukhin VA; Sibenzyme Ltd., Novosibirsk, 630117, Russia. valera@sibenzyme.ru, Kuznetsov VV, Gonchar DA, Kashirina YG, Netesova NA, Degtyarev SKh

Publikováno v: Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2010 Jan; Vol. 75 (1), pp. 63-71.

A physical map of human Alu repeats cleavage by restriction endonucleases.

Autor: Abdurashitov MA; SibEnzyme, 2/12 Ak, Timakov Str, 630117 Novosibirsk, Russia. abd@sibenzyme.ru, Tomilov VN, Chernukhin VA, Degtyarev SKh

Publikováno v: BMC genomics [BMC Genomics] 2008 Jun 26; Vol. 9, pp. 305. Date of Electronic Publication: 2008 Jun 26.

Thermostable DNA polymerase from Thermus thermophilus B35: preparation and study of a modified form of the enzyme with high affinity to ddNTP.

Autor: Akishev AG; Institute of Molecular Biology and Biophysics, Siberian Branch of the Russian Academy of Medical Sciences, Novosibirsk 630117, Russia., Rechkunova NI, Tomilova JE, Lebedeva NA, Lavrik OI, Degtyarev SKh

Publikováno v: Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2003 Dec; Vol. 68 (12), pp. 1307-12.